Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

Vivian Wilhelm, Cristián Soza, Rodgrigo Martínez, Mario Rosemblatt, Luis O. Burzio, Pablo D T Valenzuela

Resultado de la investigación: Article

30 Citas (Scopus)

Resumen

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.

Idioma originalEnglish
Páginas (desde-hasta)69-82
Número de páginas14
PublicaciónBiological Research
Volumen38
N.º1
DOI
EstadoPublished - 1 ene 2005

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
Chaperonin 60
HSP70 Heat-Shock Proteins
Salmon
Pathogens
heat shock proteins
Recombinant Proteins
recombinant proteins
salmon
immune response
Fish
pathogens
Fishes
Salmo salar
Synthetic Vaccines
Thioredoxins
Proteins
Gene encoding
proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Wilhelm, Vivian ; Soza, Cristián ; Martínez, Rodgrigo ; Rosemblatt, Mario ; Burzio, Luis O. ; Valenzuela, Pablo D T. / Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis. En: Biological Research. 2005 ; Vol. 38, N.º 1. pp. 69-82.
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abstract = "We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80{\%} homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.",
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Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis. / Wilhelm, Vivian; Soza, Cristián; Martínez, Rodgrigo; Rosemblatt, Mario; Burzio, Luis O.; Valenzuela, Pablo D T.

En: Biological Research, Vol. 38, N.º 1, 01.01.2005, p. 69-82.

Resultado de la investigación: Article

TY - JOUR

T1 - Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

AU - Wilhelm, Vivian

AU - Soza, Cristián

AU - Martínez, Rodgrigo

AU - Rosemblatt, Mario

AU - Burzio, Luis O.

AU - Valenzuela, Pablo D T

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AB - We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.

KW - dnaK

KW - groEL

KW - Hsp60

KW - Hsp70

KW - Immune response

KW - Piscirickettsia salmonis

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