Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

Vivian Wilhelm, Cristián Soza, Rodgrigo Martínez, Mario Rosemblatt, Luis O. Burzio, Pablo D T Valenzuela

Resultado de la investigación: Contribución a una revistaArtículo

32 Citas (Scopus)

Resumen

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.

Idioma originalInglés
Páginas (desde-hasta)69-82
Número de páginas14
PublicaciónBiological Research
Volumen38
N.º1
DOI
EstadoPublicada - 1 ene 2005

Áreas temáticas de ASJC Scopus

  • Bioquímica, genética y biología molecular (todo)
  • Agricultura y biología (todo)

Huella Profundice en los temas de investigación de 'Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis'. En conjunto forman una huella única.

  • Citar esto

    Wilhelm, V., Soza, C., Martínez, R., Rosemblatt, M., Burzio, L. O., & Valenzuela, P. D. T. (2005). Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis. Biological Research, 38(1), 69-82. https://doi.org/10.4067/S0716-97602005000100009