Primary and secondary experimental infestation of rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a wild rabbit: Factors determining resistance to reinfestation

Rosa Casais, Kevin P. Dalton, Javier Millán, Ana Balseiro, Álvaro Oleaga, Paloma Solano, Félix Goyache, José Miguel Prieto, Francisco Parra

Resultado de la investigación: Article

14 Citas (Scopus)

Resumen

Studies of sarcoptic mange and immunity are hampered by lack of mite sources and natural infestation models. We have investigated the clinical and pathological signs, specific IgG response and acquired immunity in naïve New Zealand White rabbits (Oryctolagus cuniculus) experimentally infested with Sarcoptes scabiei originally isolated from a clinically affected free-living European wild rabbit. Twenty rabbits were infested using two methods, direct contact for a 24. h period with a seeder rabbit simulating the natural process of infestation and application of a dressing holding approximately 1800 live mites on each hind limb (foot area) for a 24. h period. Eight weeks post infestation, rabbits were treated with ivermectin and infestation cleared. Eight weeks later seventeen previously infested and four uninfested naïve controls were then re-exposed to the same S. scabiei variety using the same methods and followed for another 8 weeks. The progress of the disease was markedly more virulent in the animals infested by contact, indicating that the effective dose of mites managing to thrive and infest each rabbit by this method was higher. Nevertheless, infestation by contact resulted in partial protection to reexposure, rabbits developed high non-protective antibody titres upon reinfestation and presented severe clinical signs. However, rabbits reinfested by dressing developed lower IgG titres, and presented high levels of resistance to reinfestation, which might be due to induction of a strong local cellular response in the inoculation point that killed the mites and resulted in a lower mite effective dose, with subsequent reduced lesion development. Statistical analysis showed that sex, method of infestation and previous exposure are key factors determining the ability of rabbits to develop immunity to this disease. The rabbit-mange model developed will allow the further study of immunity and resistance to this neglected pathogen using a natural host system.

Idioma originalEnglish
Páginas (desde-hasta)173-183
Número de páginas11
PublicaciónVeterinary Parasitology
Volumen203
N.º1-2
DOI
EstadoPublished - 1 ene 2014

Huella dactilar

Sarcoptes scabiei
Oryctolagus cuniculus
R Factors
rabbits
Rabbits
Mites
mites
immunity
Immunity
Bandages
mange
scabies
Immunoglobulin G
Mite Infestations
New Zealand White rabbit
ivermectin
Scabies
direct contact
Ivermectin
dosage

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Citar esto

Casais, Rosa ; Dalton, Kevin P. ; Millán, Javier ; Balseiro, Ana ; Oleaga, Álvaro ; Solano, Paloma ; Goyache, Félix ; Prieto, José Miguel ; Parra, Francisco. / Primary and secondary experimental infestation of rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a wild rabbit : Factors determining resistance to reinfestation. En: Veterinary Parasitology. 2014 ; Vol. 203, N.º 1-2. pp. 173-183.
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abstract = "Studies of sarcoptic mange and immunity are hampered by lack of mite sources and natural infestation models. We have investigated the clinical and pathological signs, specific IgG response and acquired immunity in na{\"i}ve New Zealand White rabbits (Oryctolagus cuniculus) experimentally infested with Sarcoptes scabiei originally isolated from a clinically affected free-living European wild rabbit. Twenty rabbits were infested using two methods, direct contact for a 24. h period with a seeder rabbit simulating the natural process of infestation and application of a dressing holding approximately 1800 live mites on each hind limb (foot area) for a 24. h period. Eight weeks post infestation, rabbits were treated with ivermectin and infestation cleared. Eight weeks later seventeen previously infested and four uninfested na{\"i}ve controls were then re-exposed to the same S. scabiei variety using the same methods and followed for another 8 weeks. The progress of the disease was markedly more virulent in the animals infested by contact, indicating that the effective dose of mites managing to thrive and infest each rabbit by this method was higher. Nevertheless, infestation by contact resulted in partial protection to reexposure, rabbits developed high non-protective antibody titres upon reinfestation and presented severe clinical signs. However, rabbits reinfested by dressing developed lower IgG titres, and presented high levels of resistance to reinfestation, which might be due to induction of a strong local cellular response in the inoculation point that killed the mites and resulted in a lower mite effective dose, with subsequent reduced lesion development. Statistical analysis showed that sex, method of infestation and previous exposure are key factors determining the ability of rabbits to develop immunity to this disease. The rabbit-mange model developed will allow the further study of immunity and resistance to this neglected pathogen using a natural host system.",
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Primary and secondary experimental infestation of rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a wild rabbit : Factors determining resistance to reinfestation. / Casais, Rosa; Dalton, Kevin P.; Millán, Javier; Balseiro, Ana; Oleaga, Álvaro; Solano, Paloma; Goyache, Félix; Prieto, José Miguel; Parra, Francisco.

En: Veterinary Parasitology, Vol. 203, N.º 1-2, 01.01.2014, p. 173-183.

Resultado de la investigación: Article

TY - JOUR

T1 - Primary and secondary experimental infestation of rabbits (Oryctolagus cuniculus) with Sarcoptes scabiei from a wild rabbit

T2 - Factors determining resistance to reinfestation

AU - Casais, Rosa

AU - Dalton, Kevin P.

AU - Millán, Javier

AU - Balseiro, Ana

AU - Oleaga, Álvaro

AU - Solano, Paloma

AU - Goyache, Félix

AU - Prieto, José Miguel

AU - Parra, Francisco

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Studies of sarcoptic mange and immunity are hampered by lack of mite sources and natural infestation models. We have investigated the clinical and pathological signs, specific IgG response and acquired immunity in naïve New Zealand White rabbits (Oryctolagus cuniculus) experimentally infested with Sarcoptes scabiei originally isolated from a clinically affected free-living European wild rabbit. Twenty rabbits were infested using two methods, direct contact for a 24. h period with a seeder rabbit simulating the natural process of infestation and application of a dressing holding approximately 1800 live mites on each hind limb (foot area) for a 24. h period. Eight weeks post infestation, rabbits were treated with ivermectin and infestation cleared. Eight weeks later seventeen previously infested and four uninfested naïve controls were then re-exposed to the same S. scabiei variety using the same methods and followed for another 8 weeks. The progress of the disease was markedly more virulent in the animals infested by contact, indicating that the effective dose of mites managing to thrive and infest each rabbit by this method was higher. Nevertheless, infestation by contact resulted in partial protection to reexposure, rabbits developed high non-protective antibody titres upon reinfestation and presented severe clinical signs. However, rabbits reinfested by dressing developed lower IgG titres, and presented high levels of resistance to reinfestation, which might be due to induction of a strong local cellular response in the inoculation point that killed the mites and resulted in a lower mite effective dose, with subsequent reduced lesion development. Statistical analysis showed that sex, method of infestation and previous exposure are key factors determining the ability of rabbits to develop immunity to this disease. The rabbit-mange model developed will allow the further study of immunity and resistance to this neglected pathogen using a natural host system.

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