Presence of RNA in the sperm nucleus

Carlos A. Pessot, Monica Brito, Jaime Figueroa, Ilona I. Concha, Alejandro Yañez, Luis O. Burzio

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

121 Citas (Scopus)

Resumen

The RNase-colloidal gold procedure for the ultrastructural localization of RNA was used for rat testis. Along with other structures, it was found that the testicular sperm nucleus was well stained. Similar labelling was observed in the nucleus of rat epididymal sperm and human sperm. The RNA was extracted from sperm and analyzed by electrophoresis on 10% polyacrylamide gel and 7 M urea. The electrophoretic profile revealed a complex set of bands ranging in size from tRNA to high molecular weight components. On the average, a content of about 0.1 pg of RNA per rat or human sperm was found.

Idioma originalInglés
Páginas (desde-hasta)272-278
Número de páginas7
PublicaciónBiochemical and Biophysical Research Communications
Volumen158
N.º1
DOI
EstadoPublicada - 16 ene. 1989
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

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