Resumen
Previous methods of heterogeneous ribonucleic acid (HnRNA) extraction yield material which "disaggregates" into small molecules. This could be the fault of either ribonuclease knicks in the polymers sustained during the extraction procedure or disaggregation into real subunits. The present communication distinguishes between these possibilities by describing an RNA extraction procedure which does not yield subunits when HnRNA is denatured. By the criteria of sedimentation through sucrose, formaldehyde, and dimethyl sulfoxide, it is estimated that the majority of the radioactivity of giant HnRNA after a 30-min pulse of [3H]uridine is associated with molecules in the range 5-10 × 106 daltons. In the electron microscope, under denaturing conditions, 84% (mass %) of giant HnRNA has a contour length of 4-9 μ corresponding to a molecular weight of about 5-10 × 106. Giant HnRNA has a "DNA-like" base composition (G + C = 46-54%) and has considerable secondary structure (ca. 60% helix conformation) as judged by its melting profile and reactivity with formaldehyde.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 2330-2338 |
| Número de páginas | 9 |
| Publicación | Biochemistry |
| Volumen | 12 |
| N.º | 12 |
| Estado | Published - 1973 |
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ASJC Scopus subject areas
- Biochemistry
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Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid. / Holmes, David S.; Bonner, James.
En: Biochemistry, Vol. 12, N.º 12, 1973, p. 2330-2338.Resultado de la investigación: Article
TY - JOUR
T1 - Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid
AU - Holmes, David S.
AU - Bonner, James
PY - 1973
Y1 - 1973
N2 - Previous methods of heterogeneous ribonucleic acid (HnRNA) extraction yield material which "disaggregates" into small molecules. This could be the fault of either ribonuclease knicks in the polymers sustained during the extraction procedure or disaggregation into real subunits. The present communication distinguishes between these possibilities by describing an RNA extraction procedure which does not yield subunits when HnRNA is denatured. By the criteria of sedimentation through sucrose, formaldehyde, and dimethyl sulfoxide, it is estimated that the majority of the radioactivity of giant HnRNA after a 30-min pulse of [3H]uridine is associated with molecules in the range 5-10 × 106 daltons. In the electron microscope, under denaturing conditions, 84% (mass %) of giant HnRNA has a contour length of 4-9 μ corresponding to a molecular weight of about 5-10 × 106. Giant HnRNA has a "DNA-like" base composition (G + C = 46-54%) and has considerable secondary structure (ca. 60% helix conformation) as judged by its melting profile and reactivity with formaldehyde.
AB - Previous methods of heterogeneous ribonucleic acid (HnRNA) extraction yield material which "disaggregates" into small molecules. This could be the fault of either ribonuclease knicks in the polymers sustained during the extraction procedure or disaggregation into real subunits. The present communication distinguishes between these possibilities by describing an RNA extraction procedure which does not yield subunits when HnRNA is denatured. By the criteria of sedimentation through sucrose, formaldehyde, and dimethyl sulfoxide, it is estimated that the majority of the radioactivity of giant HnRNA after a 30-min pulse of [3H]uridine is associated with molecules in the range 5-10 × 106 daltons. In the electron microscope, under denaturing conditions, 84% (mass %) of giant HnRNA has a contour length of 4-9 μ corresponding to a molecular weight of about 5-10 × 106. Giant HnRNA has a "DNA-like" base composition (G + C = 46-54%) and has considerable secondary structure (ca. 60% helix conformation) as judged by its melting profile and reactivity with formaldehyde.
UR - http://www.scopus.com/inward/record.url?scp=0015797002&partnerID=8YFLogxK
M3 - Article
C2 - 4710584
AN - SCOPUS:0015797002
VL - 12
SP - 2330
EP - 2338
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 12
ER -