Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella

Paulina Uribe, Daniela Fuentes, Jorge Valdés, Amir Shmaryahu, Alicia Zúñiga, David Holmes, Pablo D T Valenzuela

Resultado de la investigación: Article

31 Citas (Scopus)

Resumen

Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).

Idioma originalEnglish
Páginas (desde-hasta)692-700
Número de páginas9
PublicaciónMarine Biotechnology
Volumen10
N.º6
DOI
EstadoPublished - dic 2008

Huella dactilar

Dinoflagellida
unigenes
Poisons
Expressed Sequence Tags
expressed sequence tags
dinoflagellate
gene
shellfish culture
Genes
paralytic shellfish poisoning
aquaculture industry
gene banks
Alexandrium
genes
luminescence
Shellfish Poisoning
protist
carbohydrate metabolism
shellfish
Microalgae

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Citar esto

Uribe, P., Fuentes, D., Valdés, J., Shmaryahu, A., Zúñiga, A., Holmes, D., & Valenzuela, P. D. T. (2008). Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella. Marine Biotechnology, 10(6), 692-700. https://doi.org/10.1007/s10126-008-9107-8
Uribe, Paulina ; Fuentes, Daniela ; Valdés, Jorge ; Shmaryahu, Amir ; Zúñiga, Alicia ; Holmes, David ; Valenzuela, Pablo D T. / Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella. En: Marine Biotechnology. 2008 ; Vol. 10, N.º 6. pp. 692-700.
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abstract = "Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6{\%} of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).",
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Uribe, P, Fuentes, D, Valdés, J, Shmaryahu, A, Zúñiga, A, Holmes, D & Valenzuela, PDT 2008, 'Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella', Marine Biotechnology, vol. 10, n.º 6, pp. 692-700. https://doi.org/10.1007/s10126-008-9107-8

Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella. / Uribe, Paulina; Fuentes, Daniela; Valdés, Jorge; Shmaryahu, Amir; Zúñiga, Alicia; Holmes, David; Valenzuela, Pablo D T.

En: Marine Biotechnology, Vol. 10, N.º 6, 12.2008, p. 692-700.

Resultado de la investigación: Article

TY - JOUR

T1 - Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella

AU - Uribe, Paulina

AU - Fuentes, Daniela

AU - Valdés, Jorge

AU - Shmaryahu, Amir

AU - Zúñiga, Alicia

AU - Holmes, David

AU - Valenzuela, Pablo D T

PY - 2008/12

Y1 - 2008/12

N2 - Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).

AB - Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).

KW - Alexandrium catenella

KW - cDNA sequencing

KW - Red tide microalgae

KW - Toxic dinoflagellate

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