Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella

Paulina Uribe, Daniela Fuentes, Jorge Valdés, Amir Shmaryahu, Alicia Zúñiga, David Holmes, Pablo D T Valenzuela

Resultado de la investigación: Contribución a una revistaArtículo

33 Citas (Scopus)

Resumen

Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).

Idioma originalInglés
Páginas (desde-hasta)692-700
Número de páginas9
PublicaciónMarine Biotechnology
Volumen10
N.º6
DOI
EstadoPublicada - dic 2008

Áreas temáticas de ASJC Scopus

  • Ciencias acuáticas
  • Biotecnología

Huella Profundice en los temas de investigación de 'Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella'. En conjunto forman una huella única.

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    Uribe, P., Fuentes, D., Valdés, J., Shmaryahu, A., Zúñiga, A., Holmes, D., & Valenzuela, P. D. T. (2008). Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate Alexandrium catenella. Marine Biotechnology, 10(6), 692-700. https://doi.org/10.1007/s10126-008-9107-8