Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea

E. M. Silva, J. Valdés, A. Shmaryahu, D. Holmes, P. Valenzuela

Resultado de la investigación: Conference contribution

Resumen

The disease "gray mold" is produced by the fungus Botrytis cinerea and is responsible for great economic losses on many crops such as berries, kiwifruits, tomatoes, apples, grapes and ornamentals. A better understanding of the physiology of B. cinerea physiology can be obtained by means of genomic tools. A cDNA library was built to allow the identification of fungal genes participating in infection, pathogenicity and tolerance to pesticides. The present work describes the construction, sequencing and bioinformatic analysis of a cDNA library prepared from an isolate of Botrytis cinerea obtained from blueberries. We are interested in isolating genes related to pathogenicity in soft berries, in particular raspberries. More than 11,000 EST were sequenced, assembled and annotated by comparing them with the available data bases. The results of the bioinformatics analysis indicated that more than 50% of the sequences identified are involved in cellular metabolism, 12% are involved in transport and approximately 10% in cellular organization. Other functional categories include genes involved in response to biotic and abiotic stimuli, cell communication, cell homeostasis and development. The analysis of this library has generated new information regarding the nature of the genes involved in spore attachment, spore germination, tissue penetration and tissue invasion.

Idioma originalEnglish
Título de la publicación alojadaProceedings of the Ninth International Rubus and Ribes Symposium
Páginas197-201
Número de páginas5
Volumen777
EstadoPublished - ene 2008

Serie de la publicación

NombreActa Horticulturae
Volumen777
ISSN (versión impresa)05677572

Huella dactilar

Botrytis cinerea
cDNA libraries
genomics
bioinformatics
small fruits
pathogenicity
genes
physiology
cell communication
raspberries
spore germination
kiwifruit
blueberries
ornamental plants
grapes
homeostasis
pesticides
spores
apples
tomatoes

ASJC Scopus subject areas

  • Horticulture

Citar esto

Silva, E. M., Valdés, J., Shmaryahu, A., Holmes, D., & Valenzuela, P. (2008). Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea. En Proceedings of the Ninth International Rubus and Ribes Symposium (Vol. 777, pp. 197-201). (Acta Horticulturae; Vol. 777).
Silva, E. M. ; Valdés, J. ; Shmaryahu, A. ; Holmes, D. ; Valenzuela, P. / Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea. Proceedings of the Ninth International Rubus and Ribes Symposium. Vol. 777 2008. pp. 197-201 (Acta Horticulturae).
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abstract = "The disease {"}gray mold{"} is produced by the fungus Botrytis cinerea and is responsible for great economic losses on many crops such as berries, kiwifruits, tomatoes, apples, grapes and ornamentals. A better understanding of the physiology of B. cinerea physiology can be obtained by means of genomic tools. A cDNA library was built to allow the identification of fungal genes participating in infection, pathogenicity and tolerance to pesticides. The present work describes the construction, sequencing and bioinformatic analysis of a cDNA library prepared from an isolate of Botrytis cinerea obtained from blueberries. We are interested in isolating genes related to pathogenicity in soft berries, in particular raspberries. More than 11,000 EST were sequenced, assembled and annotated by comparing them with the available data bases. The results of the bioinformatics analysis indicated that more than 50{\%} of the sequences identified are involved in cellular metabolism, 12{\%} are involved in transport and approximately 10{\%} in cellular organization. Other functional categories include genes involved in response to biotic and abiotic stimuli, cell communication, cell homeostasis and development. The analysis of this library has generated new information regarding the nature of the genes involved in spore attachment, spore germination, tissue penetration and tissue invasion.",
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Silva, EM, Valdés, J, Shmaryahu, A, Holmes, D & Valenzuela, P 2008, Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea. En Proceedings of the Ninth International Rubus and Ribes Symposium. vol. 777, Acta Horticulturae, vol. 777, pp. 197-201.

Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea. / Silva, E. M.; Valdés, J.; Shmaryahu, A.; Holmes, D.; Valenzuela, P.

Proceedings of the Ninth International Rubus and Ribes Symposium. Vol. 777 2008. p. 197-201 (Acta Horticulturae; Vol. 777).

Resultado de la investigación: Conference contribution

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AU - Silva, E. M.

AU - Valdés, J.

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AU - Holmes, D.

AU - Valenzuela, P.

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N2 - The disease "gray mold" is produced by the fungus Botrytis cinerea and is responsible for great economic losses on many crops such as berries, kiwifruits, tomatoes, apples, grapes and ornamentals. A better understanding of the physiology of B. cinerea physiology can be obtained by means of genomic tools. A cDNA library was built to allow the identification of fungal genes participating in infection, pathogenicity and tolerance to pesticides. The present work describes the construction, sequencing and bioinformatic analysis of a cDNA library prepared from an isolate of Botrytis cinerea obtained from blueberries. We are interested in isolating genes related to pathogenicity in soft berries, in particular raspberries. More than 11,000 EST were sequenced, assembled and annotated by comparing them with the available data bases. The results of the bioinformatics analysis indicated that more than 50% of the sequences identified are involved in cellular metabolism, 12% are involved in transport and approximately 10% in cellular organization. Other functional categories include genes involved in response to biotic and abiotic stimuli, cell communication, cell homeostasis and development. The analysis of this library has generated new information regarding the nature of the genes involved in spore attachment, spore germination, tissue penetration and tissue invasion.

AB - The disease "gray mold" is produced by the fungus Botrytis cinerea and is responsible for great economic losses on many crops such as berries, kiwifruits, tomatoes, apples, grapes and ornamentals. A better understanding of the physiology of B. cinerea physiology can be obtained by means of genomic tools. A cDNA library was built to allow the identification of fungal genes participating in infection, pathogenicity and tolerance to pesticides. The present work describes the construction, sequencing and bioinformatic analysis of a cDNA library prepared from an isolate of Botrytis cinerea obtained from blueberries. We are interested in isolating genes related to pathogenicity in soft berries, in particular raspberries. More than 11,000 EST were sequenced, assembled and annotated by comparing them with the available data bases. The results of the bioinformatics analysis indicated that more than 50% of the sequences identified are involved in cellular metabolism, 12% are involved in transport and approximately 10% in cellular organization. Other functional categories include genes involved in response to biotic and abiotic stimuli, cell communication, cell homeostasis and development. The analysis of this library has generated new information regarding the nature of the genes involved in spore attachment, spore germination, tissue penetration and tissue invasion.

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Silva EM, Valdés J, Shmaryahu A, Holmes D, Valenzuela P. Preliminary genomic and functional analysis of a cDNA library from Botrytis cinerea. En Proceedings of the Ninth International Rubus and Ribes Symposium. Vol. 777. 2008. p. 197-201. (Acta Horticulturae).