Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans

H. Osorio, J. P. Cárdenas, J. Valdés, D. S. Holmes

Resultado de la investigación: Conference contribution

7 Citas (Scopus)

Resumen

Efficient bioleaching requires adequate access to oxygen to drive the biochemical reactions that underpin iron and sulfur oxidation and ultimately copper solubilization. However, microaerophilic or anaerobic conditions may occur in certain parts of the heap, especially in areas of intense microbial activity or in biofilms where oxygen gradients occur. Microaerophilic conditions have also been detected in pristine acidic environments and in abandoned bioleaching operations. An important microorganism in bioleaching at ambient temperatures is the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans. In addition to its well established role in oxidative processes, it has been shown to be capable of reducing metals such as iron and sulfur, and the products of these reactions may promote passivation of mineral surfaces and impede efficient solubilization of copper. In an effort to advance our understanding of the genetic and physiological basis of anaerobic metabolism, gene clusters controlled by the master anaerobic transcriptional regulator FNR were predicted in the genome of A. ferrooxidans using bioinformatics techniques. These clusters were found to be associated with energy metabolism, nitrogen fixation and carbohydrate metabolism. The results not only support previous evidence for proposed anaerobic metabolic pathways but also identify new genetic components and pathways that may be important for anaerobic or microaerophilic growth of this microorganism.

Idioma originalEnglish
Título de la publicación alojadaBiohydrometallurgy 2009
Páginas195-198
Número de páginas4
Volumen71-73
DOI
EstadoPublished - 2009
Evento18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duración: 13 sep 200917 sep 2009

Serie de la publicación

NombreAdvanced Materials Research
Volumen71-73
ISSN (versión impresa)10226680

Other

Other18th International Biohydrometallurgy Symposium, IBS 2009
PaísArgentina
CiudadBariloche
Período13/09/0917/09/09

Huella dactilar

Bioleaching
Genes
Microorganisms
Sulfur
Nitrogen fixation
Iron
Copper
Oxygen
Biofilms
Bioinformatics
Metabolism
Passivation
Bacteria
Minerals
Oxidation
Metals
Metabolic Networks and Pathways
Temperature

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Osorio, H., Cárdenas, J. P., Valdés, J., & Holmes, D. S. (2009). Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009 (Vol. 71-73, pp. 195-198). (Advanced Materials Research; Vol. 71-73). https://doi.org/10.4028/www.scientific.net/AMR.71-73.195
Osorio, H. ; Cárdenas, J. P. ; Valdés, J. ; Holmes, D. S. / Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans. Biohydrometallurgy 2009. Vol. 71-73 2009. pp. 195-198 (Advanced Materials Research).
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abstract = "Efficient bioleaching requires adequate access to oxygen to drive the biochemical reactions that underpin iron and sulfur oxidation and ultimately copper solubilization. However, microaerophilic or anaerobic conditions may occur in certain parts of the heap, especially in areas of intense microbial activity or in biofilms where oxygen gradients occur. Microaerophilic conditions have also been detected in pristine acidic environments and in abandoned bioleaching operations. An important microorganism in bioleaching at ambient temperatures is the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans. In addition to its well established role in oxidative processes, it has been shown to be capable of reducing metals such as iron and sulfur, and the products of these reactions may promote passivation of mineral surfaces and impede efficient solubilization of copper. In an effort to advance our understanding of the genetic and physiological basis of anaerobic metabolism, gene clusters controlled by the master anaerobic transcriptional regulator FNR were predicted in the genome of A. ferrooxidans using bioinformatics techniques. These clusters were found to be associated with energy metabolism, nitrogen fixation and carbohydrate metabolism. The results not only support previous evidence for proposed anaerobic metabolic pathways but also identify new genetic components and pathways that may be important for anaerobic or microaerophilic growth of this microorganism.",
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Osorio, H, Cárdenas, JP, Valdés, J & Holmes, DS 2009, Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009. vol. 71-73, Advanced Materials Research, vol. 71-73, pp. 195-198, 18th International Biohydrometallurgy Symposium, IBS 2009, Bariloche, Argentina, 13/09/09. https://doi.org/10.4028/www.scientific.net/AMR.71-73.195

Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans. / Osorio, H.; Cárdenas, J. P.; Valdés, J.; Holmes, D. S.

Biohydrometallurgy 2009. Vol. 71-73 2009. p. 195-198 (Advanced Materials Research; Vol. 71-73).

Resultado de la investigación: Conference contribution

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T1 - Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans

AU - Osorio, H.

AU - Cárdenas, J. P.

AU - Valdés, J.

AU - Holmes, D. S.

PY - 2009

Y1 - 2009

N2 - Efficient bioleaching requires adequate access to oxygen to drive the biochemical reactions that underpin iron and sulfur oxidation and ultimately copper solubilization. However, microaerophilic or anaerobic conditions may occur in certain parts of the heap, especially in areas of intense microbial activity or in biofilms where oxygen gradients occur. Microaerophilic conditions have also been detected in pristine acidic environments and in abandoned bioleaching operations. An important microorganism in bioleaching at ambient temperatures is the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans. In addition to its well established role in oxidative processes, it has been shown to be capable of reducing metals such as iron and sulfur, and the products of these reactions may promote passivation of mineral surfaces and impede efficient solubilization of copper. In an effort to advance our understanding of the genetic and physiological basis of anaerobic metabolism, gene clusters controlled by the master anaerobic transcriptional regulator FNR were predicted in the genome of A. ferrooxidans using bioinformatics techniques. These clusters were found to be associated with energy metabolism, nitrogen fixation and carbohydrate metabolism. The results not only support previous evidence for proposed anaerobic metabolic pathways but also identify new genetic components and pathways that may be important for anaerobic or microaerophilic growth of this microorganism.

AB - Efficient bioleaching requires adequate access to oxygen to drive the biochemical reactions that underpin iron and sulfur oxidation and ultimately copper solubilization. However, microaerophilic or anaerobic conditions may occur in certain parts of the heap, especially in areas of intense microbial activity or in biofilms where oxygen gradients occur. Microaerophilic conditions have also been detected in pristine acidic environments and in abandoned bioleaching operations. An important microorganism in bioleaching at ambient temperatures is the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans. In addition to its well established role in oxidative processes, it has been shown to be capable of reducing metals such as iron and sulfur, and the products of these reactions may promote passivation of mineral surfaces and impede efficient solubilization of copper. In an effort to advance our understanding of the genetic and physiological basis of anaerobic metabolism, gene clusters controlled by the master anaerobic transcriptional regulator FNR were predicted in the genome of A. ferrooxidans using bioinformatics techniques. These clusters were found to be associated with energy metabolism, nitrogen fixation and carbohydrate metabolism. The results not only support previous evidence for proposed anaerobic metabolic pathways but also identify new genetic components and pathways that may be important for anaerobic or microaerophilic growth of this microorganism.

KW - Acidithiobacillus

KW - Anaerobic growth

KW - Bioinformatics

KW - Fermentation

KW - FNR

KW - Genomics

KW - N fixation

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DO - 10.4028/www.scientific.net/AMR.71-73.195

M3 - Conference contribution

AN - SCOPUS:72449169512

SN - 0878493220

SN - 9780878493227

VL - 71-73

T3 - Advanced Materials Research

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BT - Biohydrometallurgy 2009

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Osorio H, Cárdenas JP, Valdés J, Holmes DS. Prediction of FNR regulated genes and metabolic pathways potentially involved in anaerobic growth of Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009. Vol. 71-73. 2009. p. 195-198. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.71-73.195