Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway

J. P. Cárdenas, V. Martínez, P. Covarrubias, D. S. Holmes, R. Quatrini

Resultado de la investigación: Conference contribution

3 Citas (Scopus)

Resumen

Previous physiological studies of the genus Ferroplasma have indicated that these microorganisms are capable of fixing CO2 in the presence of ferrous iron and low concentrations of yeast extract. Analysis of the gene complement of Ferroplasma acidarmanus fer1 and two partial genomes of Ferroplasma type I and II derived from the Iron Mountain acid mine drainage metagenome revealed the absence of several functional marker genes encoding key enzymes of three know alternative CO2 fixation routes present in archaea, i.e. the 3-hydroxypropionate cycle, the Ljungdahl-Wood pathway and the reverse TCA cycle. It is thus intriguing how these chemoautotrophic archaeal species deal with their requirements for carbon and suggests that they might have a distinct CO2 fixation route, as yet unreported. Using comparative genomics and metabolic reconstruction strategies, a putative pathway was detected for C1 fixation consisting of four main steps: 1) conversion of carbon monoxide to carbon dioxide with gain of energy and/or 2) reduction of carbon dioxide to formate, 3) incorporation of formate to tetrahydrofolate and 4) donation of the carbon moiety of tetrahydrofolate to glycine to produce serine. Steps 1 to 3 involve enzymes that correspond to some of the Ljungdahl-Wood pathway proteins, whereas step 4 resembles the well known "serine cycle", utilized by methylotrophic microorganisms for formaldehyde fixation. Thus, this chimaeric pathway might represent the missing carbon fixation route in Ferroplasmatales. Herein, we discuss the implications of these findings in the context of central carbon metabolism requirements for biomass production in acidic environments.

Idioma originalEnglish
Título de la publicación alojadaBiohydrometallurgy 2009
Páginas219-222
Número de páginas4
Volumen71-73
DOI
EstadoPublished - 2009
Evento18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duración: 13 sep 200917 sep 2009

Serie de la publicación

NombreAdvanced Materials Research
Volumen71-73
ISSN (versión impresa)10226680

Other

Other18th International Biohydrometallurgy Symposium, IBS 2009
PaísArgentina
CiudadBariloche
Período13/09/0917/09/09

Huella dactilar

Carbon
Microorganisms
Carbon dioxide
Wood
Enzymes
Genes
Iron
Gene encoding
Formaldehyde
Metabolism
Carbon monoxide
Yeast
Drainage
Amino acids
Biomass
Proteins
Acids
Genomics

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Cárdenas, J. P., Martínez, V., Covarrubias, P., Holmes, D. S., & Quatrini, R. (2009). Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway. En Biohydrometallurgy 2009 (Vol. 71-73, pp. 219-222). (Advanced Materials Research; Vol. 71-73). https://doi.org/10.4028/www.scientific.net/AMR.71-73.219
Cárdenas, J. P. ; Martínez, V. ; Covarrubias, P. ; Holmes, D. S. ; Quatrini, R. / Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway. Biohydrometallurgy 2009. Vol. 71-73 2009. pp. 219-222 (Advanced Materials Research).
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Cárdenas, JP, Martínez, V, Covarrubias, P, Holmes, DS & Quatrini, R 2009, Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway. En Biohydrometallurgy 2009. vol. 71-73, Advanced Materials Research, vol. 71-73, pp. 219-222, 18th International Biohydrometallurgy Symposium, IBS 2009, Bariloche, Argentina, 13/09/09. https://doi.org/10.4028/www.scientific.net/AMR.71-73.219

Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway. / Cárdenas, J. P.; Martínez, V.; Covarrubias, P.; Holmes, D. S.; Quatrini, R.

Biohydrometallurgy 2009. Vol. 71-73 2009. p. 219-222 (Advanced Materials Research; Vol. 71-73).

Resultado de la investigación: Conference contribution

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AU - Cárdenas, J. P.

AU - Martínez, V.

AU - Covarrubias, P.

AU - Holmes, D. S.

AU - Quatrini, R.

PY - 2009

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AB - Previous physiological studies of the genus Ferroplasma have indicated that these microorganisms are capable of fixing CO2 in the presence of ferrous iron and low concentrations of yeast extract. Analysis of the gene complement of Ferroplasma acidarmanus fer1 and two partial genomes of Ferroplasma type I and II derived from the Iron Mountain acid mine drainage metagenome revealed the absence of several functional marker genes encoding key enzymes of three know alternative CO2 fixation routes present in archaea, i.e. the 3-hydroxypropionate cycle, the Ljungdahl-Wood pathway and the reverse TCA cycle. It is thus intriguing how these chemoautotrophic archaeal species deal with their requirements for carbon and suggests that they might have a distinct CO2 fixation route, as yet unreported. Using comparative genomics and metabolic reconstruction strategies, a putative pathway was detected for C1 fixation consisting of four main steps: 1) conversion of carbon monoxide to carbon dioxide with gain of energy and/or 2) reduction of carbon dioxide to formate, 3) incorporation of formate to tetrahydrofolate and 4) donation of the carbon moiety of tetrahydrofolate to glycine to produce serine. Steps 1 to 3 involve enzymes that correspond to some of the Ljungdahl-Wood pathway proteins, whereas step 4 resembles the well known "serine cycle", utilized by methylotrophic microorganisms for formaldehyde fixation. Thus, this chimaeric pathway might represent the missing carbon fixation route in Ferroplasmatales. Herein, we discuss the implications of these findings in the context of central carbon metabolism requirements for biomass production in acidic environments.

KW - Bioinformatics

KW - CO fixation

KW - Ferroplasma

KW - Genomics

KW - Metabolic reconstruction

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Cárdenas JP, Martínez V, Covarrubias P, Holmes DS, Quatrini R. Predicted CO/CO2 fixation in Ferroplasma spp. via a novel chimaeric pathway. En Biohydrometallurgy 2009. Vol. 71-73. 2009. p. 219-222. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.71-73.219