Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi

Susan M. Bueno, Carlos A. Santiviago, Alejandro A. Murillo, Juan A. Fuentes, A. Nicole Trombert, Paula I. Rodas, Philip Youderian, Guido C. Mora

Resultado de la investigación: Article

49 Citas (Scopus)

Resumen

The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains 151 potential open reading frames, and includes the viaB operon involved in the synthesis of Vi antigen. Some clinical isolates of S. enterica serovar Typhi are missing the entire SPI7, due to its precise excision; these strains have lost the ability to produce Vi antigen, are resistant to phage Vi-II, and invade a human epithelial cell line more rapidly. Excision of SPI7 occurs spontaneously in a clinical isolate of S. enterica serovar Typhi when it is grown in the laboratory, leaves an intact copy of the pheU gene at its novel join point, and results in the same three phenotypic consequences. SPI7 is an unstable genetic element, probably an intermediate in the pathway of lateral transfer of such pathogenicity islands among enteric gram-negative bacteria.

Idioma originalEnglish
Páginas (desde-hasta)3202-3213
Número de páginas12
PublicaciónJournal of Bacteriology
Volumen186
N.º10
DOI
EstadoPublished - may 2004

Huella dactilar

Genomic Islands
Salmonella typhi
Antigens
Nucleic Acid Repetitive Sequences
Enterobacteriaceae
Operon
Transfer RNA
Gram-Negative Bacteria
Bacteriophages
Open Reading Frames
Genes
Epithelial Cells
Cell Line
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Molecular Biology

Citar esto

Bueno, S. M., Santiviago, C. A., Murillo, A. A., Fuentes, J. A., Trombert, A. N., Rodas, P. I., ... Mora, G. C. (2004). Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi. Journal of Bacteriology, 186(10), 3202-3213. https://doi.org/10.1128/JB.186.10.3202-3213.2004
Bueno, Susan M. ; Santiviago, Carlos A. ; Murillo, Alejandro A. ; Fuentes, Juan A. ; Trombert, A. Nicole ; Rodas, Paula I. ; Youderian, Philip ; Mora, Guido C. / Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi. En: Journal of Bacteriology. 2004 ; Vol. 186, N.º 10. pp. 3202-3213.
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abstract = "The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains 151 potential open reading frames, and includes the viaB operon involved in the synthesis of Vi antigen. Some clinical isolates of S. enterica serovar Typhi are missing the entire SPI7, due to its precise excision; these strains have lost the ability to produce Vi antigen, are resistant to phage Vi-II, and invade a human epithelial cell line more rapidly. Excision of SPI7 occurs spontaneously in a clinical isolate of S. enterica serovar Typhi when it is grown in the laboratory, leaves an intact copy of the pheU gene at its novel join point, and results in the same three phenotypic consequences. SPI7 is an unstable genetic element, probably an intermediate in the pathway of lateral transfer of such pathogenicity islands among enteric gram-negative bacteria.",
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Bueno, SM, Santiviago, CA, Murillo, AA, Fuentes, JA, Trombert, AN, Rodas, PI, Youderian, P & Mora, GC 2004, 'Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi', Journal of Bacteriology, vol. 186, n.º 10, pp. 3202-3213. https://doi.org/10.1128/JB.186.10.3202-3213.2004

Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi. / Bueno, Susan M.; Santiviago, Carlos A.; Murillo, Alejandro A.; Fuentes, Juan A.; Trombert, A. Nicole; Rodas, Paula I.; Youderian, Philip; Mora, Guido C.

En: Journal of Bacteriology, Vol. 186, N.º 10, 05.2004, p. 3202-3213.

Resultado de la investigación: Article

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AU - Bueno, Susan M.

AU - Santiviago, Carlos A.

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AU - Fuentes, Juan A.

AU - Trombert, A. Nicole

AU - Rodas, Paula I.

AU - Youderian, Philip

AU - Mora, Guido C.

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