Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition

Vanesa Nahirñak, Natalia Inés Almasia, Paula Virginia Fernandez, Horacio Esteban Hopp, José Manuel Estevez, Fernando Carrari, Cecilia Vazquez-Rovere

Resultado de la investigación: Article

41 Citas (Scopus)

Resumen

Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70% to 90% increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense.

Idioma originalEnglish
Páginas (desde-hasta)252-263
Número de páginas12
PublicaciónPlant Physiology
Volumen158
N.º1
DOI
EstadoPublished - 1 ene 2012
Publicado de forma externa

Huella dactilar

Gene Silencing
cell wall components
gene silencing
Solanum tuberosum
Cell Division
Cell Wall
cell division
potatoes
metabolism
leaves
Arabidopsis Proteins
Fourier Transform Infrared Spectroscopy
Metabolic Networks and Pathways
Green Fluorescent Proteins
Cell Size
Growth and Development
Epidermis
Confocal Microscopy
Gas Chromatography
Tobacco

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Citar esto

Nahirñak, V., Almasia, N. I., Fernandez, P. V., Hopp, H. E., Estevez, J. M., Carrari, F., & Vazquez-Rovere, C. (2012). Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition. Plant Physiology, 158(1), 252-263. https://doi.org/10.1104/pp.111.186544
Nahirñak, Vanesa ; Almasia, Natalia Inés ; Fernandez, Paula Virginia ; Hopp, Horacio Esteban ; Estevez, José Manuel ; Carrari, Fernando ; Vazquez-Rovere, Cecilia. / Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition. En: Plant Physiology. 2012 ; Vol. 158, N.º 1. pp. 252-263.
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abstract = "Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a transgenic approach was used to study the role of SN1 in planta. Even when overexpressing SN1, potato lines did not show remarkable morphological differences from the wild type; SN1 silencing resulted in reduced height, which was accompanied by an overall reduction in leaf size and severe alterations of leaf shape. Analysis of the adaxial epidermis of mature leaves revealed that silenced lines had 70{\%} to 90{\%} increases in mean cell size with respect to wild-type leaves. Consequently, the number of epidermal cells was significantly reduced in these lines. Confocal microscopy analysis after agroinfiltration of Nicotiana benthamiana leaves showed that SN1-green fluorescent protein fusion protein was localized in plasma membrane, and bimolecular fluorescence complementation assays revealed that SN1 self-interacted in vivo. We further focused our study on leaf metabolism by applying a combination of gas chromatography coupled to mass spectrometry, Fourier transform infrared spectroscopy, and spectrophotometric techniques. These targeted analyses allowed a detailed examination of the changes occurring in 46 intermediate compounds from primary metabolic pathways and in seven cell wall constituents. We demonstrated that SN1 silencing affects cell division, leaf primary metabolism, and cell wall composition in potato plants, suggesting that SN1 has additional roles in growth and development beyond its previously assigned role in plant defense.",
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Nahirñak, V, Almasia, NI, Fernandez, PV, Hopp, HE, Estevez, JM, Carrari, F & Vazquez-Rovere, C 2012, 'Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition', Plant Physiology, vol. 158, n.º 1, pp. 252-263. https://doi.org/10.1104/pp.111.186544

Potato Snakin-1 gene silencing affects cell division, primary metabolism, and cell wall composition. / Nahirñak, Vanesa; Almasia, Natalia Inés; Fernandez, Paula Virginia; Hopp, Horacio Esteban; Estevez, José Manuel; Carrari, Fernando; Vazquez-Rovere, Cecilia.

En: Plant Physiology, Vol. 158, N.º 1, 01.01.2012, p. 252-263.

Resultado de la investigación: Article

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AU - Nahirñak, Vanesa

AU - Almasia, Natalia Inés

AU - Fernandez, Paula Virginia

AU - Hopp, Horacio Esteban

AU - Estevez, José Manuel

AU - Carrari, Fernando

AU - Vazquez-Rovere, Cecilia

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