Polysome-like structures in the chromatoid body of rat spermatids

Jaime Figueroa, Luis O. Burzio

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

37 Citas (Scopus)

Resumen

A procedure for isolating the chromatoid body from the testis of 40-day-old rats was developed. Electron-microscopical analysis indicated that about 70% of the isolated organelles were chromatoid bodies, while the remaining structures corresponded to dense bodies and probably to satellites. Negative staining of the isolated organelles revealed the presence of polysome-like structures in about 20% of the chromatoid bodies suggesting that the polysomes were not due to contamination with cytoplasmic polysomes. Moreover, the presence of RNA in the stroma of the chromatoid body was confirmed by RNAse-gold staining. Preliminary electrophoretic analysis of the RNA extracted from the organelles revealed the presence of a complex population of RNAs including 5.8 and 5 S ribosomal RNAs but no tRNA.

Idioma originalInglés
Páginas (desde-hasta)575-579
Número de páginas5
PublicaciónCell and Tissue Research
Volumen291
N.º3
DOI
EstadoPublicada - 1998
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Patología y medicina forense
  • Histología
  • Biología celular

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