Poly(ADP‐ribosylation) of atypical CS histone variants is required for the progression of S phase in early embryos of sea urchins

M. Imschenetzky, M. Montecino, M. Puchi

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

The patterns of poly(ADP‐ribosylation) in vivo of CS (cleavage stage) histone variants were compared in sea urchin zygotes at the entrance and the exit of S1 and S2 in the initial developmental cell cycles. This post‐translational modification was detected by Western immunoblots with rabbit sera anti‐poly(ADP‐ribose) that was principally reactive against ADP‐ribose polymers and slightly against ADP‐ribose oligomers. The effect of 3 aminobenzamide (3‐ABA), an inhibitor of the poly(ADP‐ribose) synthetase, on S phase progression was determined in vivo by measuring the incorporation of 3H thymidine into DNA. The results obtained indicate that the CS histone variants are poly(ADP‐ribosylated) in a cell cycle dependent manner. A significantly positive reaction of several CS variants with sera anti‐poly(ADP‐ribose) was found at the entrance into S phase, which decreases after its completion. The incubation of zygotes in 3‐ABA inhibited the poly(ADP‐ribosylation) of CS variants and prevented both the progression of the first S phase and the first cleavage division. These observations suggest that the poly(ADP‐ribosylation) of atypical CS histone variants is relevant for initiation of sea urchin development and is required for embryonic DNA replication.

Idioma originalEnglish
Páginas (desde-hasta)234-241
Número de páginas8
PublicaciónJournal of Cellular Biochemistry
Volumen46
N.º3
DOI
EstadoPublished - 1991

Huella dactilar

Adenosine Diphosphate Ribose
Sea Urchins
S Phase
Histones
Embryonic Structures
Zygote
Cell Cycle
Cells
DNA
Post Translational Protein Processing
Ligases
Serum
DNA Replication
Oligomers
Thymidine
Polymers
Western Blotting
Rabbits

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

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title = "Poly(ADP‐ribosylation) of atypical CS histone variants is required for the progression of S phase in early embryos of sea urchins",
abstract = "The patterns of poly(ADP‐ribosylation) in vivo of CS (cleavage stage) histone variants were compared in sea urchin zygotes at the entrance and the exit of S1 and S2 in the initial developmental cell cycles. This post‐translational modification was detected by Western immunoblots with rabbit sera anti‐poly(ADP‐ribose) that was principally reactive against ADP‐ribose polymers and slightly against ADP‐ribose oligomers. The effect of 3 aminobenzamide (3‐ABA), an inhibitor of the poly(ADP‐ribose) synthetase, on S phase progression was determined in vivo by measuring the incorporation of 3H thymidine into DNA. The results obtained indicate that the CS histone variants are poly(ADP‐ribosylated) in a cell cycle dependent manner. A significantly positive reaction of several CS variants with sera anti‐poly(ADP‐ribose) was found at the entrance into S phase, which decreases after its completion. The incubation of zygotes in 3‐ABA inhibited the poly(ADP‐ribosylation) of CS variants and prevented both the progression of the first S phase and the first cleavage division. These observations suggest that the poly(ADP‐ribosylation) of atypical CS histone variants is relevant for initiation of sea urchin development and is required for embryonic DNA replication.",
keywords = "3 aminobenzamide, ADP‐ribosylation, cell cycle, CS histone variants, sea urchin zygotes",
author = "M. Imschenetzky and M. Montecino and M. Puchi",
year = "1991",
doi = "10.1002/jcb.240460306",
language = "English",
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journal = "Journal of Cellular Biochemistry",
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TY - JOUR

T1 - Poly(ADP‐ribosylation) of atypical CS histone variants is required for the progression of S phase in early embryos of sea urchins

AU - Imschenetzky, M.

AU - Montecino, M.

AU - Puchi, M.

PY - 1991

Y1 - 1991

N2 - The patterns of poly(ADP‐ribosylation) in vivo of CS (cleavage stage) histone variants were compared in sea urchin zygotes at the entrance and the exit of S1 and S2 in the initial developmental cell cycles. This post‐translational modification was detected by Western immunoblots with rabbit sera anti‐poly(ADP‐ribose) that was principally reactive against ADP‐ribose polymers and slightly against ADP‐ribose oligomers. The effect of 3 aminobenzamide (3‐ABA), an inhibitor of the poly(ADP‐ribose) synthetase, on S phase progression was determined in vivo by measuring the incorporation of 3H thymidine into DNA. The results obtained indicate that the CS histone variants are poly(ADP‐ribosylated) in a cell cycle dependent manner. A significantly positive reaction of several CS variants with sera anti‐poly(ADP‐ribose) was found at the entrance into S phase, which decreases after its completion. The incubation of zygotes in 3‐ABA inhibited the poly(ADP‐ribosylation) of CS variants and prevented both the progression of the first S phase and the first cleavage division. These observations suggest that the poly(ADP‐ribosylation) of atypical CS histone variants is relevant for initiation of sea urchin development and is required for embryonic DNA replication.

AB - The patterns of poly(ADP‐ribosylation) in vivo of CS (cleavage stage) histone variants were compared in sea urchin zygotes at the entrance and the exit of S1 and S2 in the initial developmental cell cycles. This post‐translational modification was detected by Western immunoblots with rabbit sera anti‐poly(ADP‐ribose) that was principally reactive against ADP‐ribose polymers and slightly against ADP‐ribose oligomers. The effect of 3 aminobenzamide (3‐ABA), an inhibitor of the poly(ADP‐ribose) synthetase, on S phase progression was determined in vivo by measuring the incorporation of 3H thymidine into DNA. The results obtained indicate that the CS histone variants are poly(ADP‐ribosylated) in a cell cycle dependent manner. A significantly positive reaction of several CS variants with sera anti‐poly(ADP‐ribose) was found at the entrance into S phase, which decreases after its completion. The incubation of zygotes in 3‐ABA inhibited the poly(ADP‐ribosylation) of CS variants and prevented both the progression of the first S phase and the first cleavage division. These observations suggest that the poly(ADP‐ribosylation) of atypical CS histone variants is relevant for initiation of sea urchin development and is required for embryonic DNA replication.

KW - 3 aminobenzamide

KW - ADP‐ribosylation

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KW - CS histone variants

KW - sea urchin zygotes

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