Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout

Verónica Rojas, Norbel Galanti, Niels C. Bols, Verónica Jiménez, Rodolfo Paredes, Sergio H. Marshall

Resultado de la investigación: Article

27 Citas (Scopus)

Resumen

Piscirickettsia salmonis is the etiologic agent of the salmonid rickettsial septicemia (SRS) which causes significant losses in salmon production in Chile and other and in other regions in the southern hemisphere. As the killing of phagocytes is an important pathogenic mechanism for other bacteria to establish infections in vertebrates, we investigated whether P. salmonis kills trout macrophages by apoptosis. Apoptosis in infected macrophages was demonstrated by techniques based on morphological changes and host cell DNA fragmentation. Transmission electron microcopy showed classic apoptotic characteristics and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling showed fragmented DNA. Programmed cell death type I was further confirmed by increased binding of annexin V to externalized phosphatidylserine in infected macrophages. Moreover, significant increases of caspase 3 activation were detected in infected cells and treatment with caspase inhibitor caused a decrease in levels of apoptosis. This is the first evidence that P. salmonis induces cell death in trout macrophages. This could lead to bacterial survival and evasion of the host immune response and play an important role in the establishment of infection in the host.

Idioma originalEnglish
Páginas (desde-hasta)468-476
Número de páginas9
PublicaciónJournal of Cellular Biochemistry
Volumen110
N.º2
DOI
EstadoPublished - 15 may 2010

Huella dactilar

Piscirickettsia
Macrophages
Oncorhynchus mykiss
Monocytes
Apoptosis
Trout
Immune Evasion
Caspase Inhibitors
DNA Nucleotidylexotransferase
Chile
Salmon
Annexin A5
Phosphatidylserines
DNA
DNA Fragmentation
Cell death
Phagocytes
Infection
Caspase 3
Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Rojas, Verónica ; Galanti, Norbel ; Bols, Niels C. ; Jiménez, Verónica ; Paredes, Rodolfo ; Marshall, Sergio H. / Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout. En: Journal of Cellular Biochemistry. 2010 ; Vol. 110, N.º 2. pp. 468-476.
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abstract = "Piscirickettsia salmonis is the etiologic agent of the salmonid rickettsial septicemia (SRS) which causes significant losses in salmon production in Chile and other and in other regions in the southern hemisphere. As the killing of phagocytes is an important pathogenic mechanism for other bacteria to establish infections in vertebrates, we investigated whether P. salmonis kills trout macrophages by apoptosis. Apoptosis in infected macrophages was demonstrated by techniques based on morphological changes and host cell DNA fragmentation. Transmission electron microcopy showed classic apoptotic characteristics and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling showed fragmented DNA. Programmed cell death type I was further confirmed by increased binding of annexin V to externalized phosphatidylserine in infected macrophages. Moreover, significant increases of caspase 3 activation were detected in infected cells and treatment with caspase inhibitor caused a decrease in levels of apoptosis. This is the first evidence that P. salmonis induces cell death in trout macrophages. This could lead to bacterial survival and evasion of the host immune response and play an important role in the establishment of infection in the host.",
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Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout. / Rojas, Verónica; Galanti, Norbel; Bols, Niels C.; Jiménez, Verónica; Paredes, Rodolfo; Marshall, Sergio H.

En: Journal of Cellular Biochemistry, Vol. 110, N.º 2, 15.05.2010, p. 468-476.

Resultado de la investigación: Article

TY - JOUR

T1 - Piscirickettsia salmonis induces apoptosis in macrophages and monocyte-like cells from rainbow trout

AU - Rojas, Verónica

AU - Galanti, Norbel

AU - Bols, Niels C.

AU - Jiménez, Verónica

AU - Paredes, Rodolfo

AU - Marshall, Sergio H.

PY - 2010/5/15

Y1 - 2010/5/15

N2 - Piscirickettsia salmonis is the etiologic agent of the salmonid rickettsial septicemia (SRS) which causes significant losses in salmon production in Chile and other and in other regions in the southern hemisphere. As the killing of phagocytes is an important pathogenic mechanism for other bacteria to establish infections in vertebrates, we investigated whether P. salmonis kills trout macrophages by apoptosis. Apoptosis in infected macrophages was demonstrated by techniques based on morphological changes and host cell DNA fragmentation. Transmission electron microcopy showed classic apoptotic characteristics and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling showed fragmented DNA. Programmed cell death type I was further confirmed by increased binding of annexin V to externalized phosphatidylserine in infected macrophages. Moreover, significant increases of caspase 3 activation were detected in infected cells and treatment with caspase inhibitor caused a decrease in levels of apoptosis. This is the first evidence that P. salmonis induces cell death in trout macrophages. This could lead to bacterial survival and evasion of the host immune response and play an important role in the establishment of infection in the host.

AB - Piscirickettsia salmonis is the etiologic agent of the salmonid rickettsial septicemia (SRS) which causes significant losses in salmon production in Chile and other and in other regions in the southern hemisphere. As the killing of phagocytes is an important pathogenic mechanism for other bacteria to establish infections in vertebrates, we investigated whether P. salmonis kills trout macrophages by apoptosis. Apoptosis in infected macrophages was demonstrated by techniques based on morphological changes and host cell DNA fragmentation. Transmission electron microcopy showed classic apoptotic characteristics and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling showed fragmented DNA. Programmed cell death type I was further confirmed by increased binding of annexin V to externalized phosphatidylserine in infected macrophages. Moreover, significant increases of caspase 3 activation were detected in infected cells and treatment with caspase inhibitor caused a decrease in levels of apoptosis. This is the first evidence that P. salmonis induces cell death in trout macrophages. This could lead to bacterial survival and evasion of the host immune response and play an important role in the establishment of infection in the host.

KW - Apoptosis

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