Peptides: An analytical method for their resolution by polyacrylamide gel electrophoresis applicable to a wide range of sizes and solubilities

Mario S. Rosemblatt, Michael N. Margolies, L. Edward Cannon, Edgar Haber

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

Polyacrylamide gel electrophoresis of Fluorescamine-modified peptides is a rapid (90 min), sensitive (<1 nmole), and reproducible method which may be used to resolve peptides without the limitations with respect to molecular size or water solubility found in other analytical systems. Fluorescamine-modified peptides, ranging in size from 3 to 215 amino acids, have been examined in a variety of buffer systems between pH 2.3 and 9.8 and gel concentrations varying between 4% and 16% acrylamide. Similar peptides were examined by high-voltage paper electrophoresis and detected by Fluorescamine. Peptides of low solubility have been examined in the presence of urea. The method reported here was used to resolve small peptides differing in size by a single amino acid, as well as peptides of the same size differing only by a sinle charge. It was successfully employed as a criterion for homogeneity of peptides in the course of purification for amino acid sequence analysis.

Idioma originalEnglish
Páginas (desde-hasta)321-330
Número de páginas10
PublicaciónAnalytical Biochemistry
Volumen65
N.º1-2
DOI
EstadoPublished - 12 may 1975

Huella dactilar

Electrophoresis
Solubility
Polyacrylamide Gel Electrophoresis
Peptides
Fluorescamine
Amino Acids
Paper Electrophoresis
polyacrylamide gels
Acrylamide
Protein Sequence Analysis
Purification
Urea
Buffers
Gels
Water
Electric potential

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

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abstract = "Polyacrylamide gel electrophoresis of Fluorescamine-modified peptides is a rapid (90 min), sensitive (<1 nmole), and reproducible method which may be used to resolve peptides without the limitations with respect to molecular size or water solubility found in other analytical systems. Fluorescamine-modified peptides, ranging in size from 3 to 215 amino acids, have been examined in a variety of buffer systems between pH 2.3 and 9.8 and gel concentrations varying between 4{\%} and 16{\%} acrylamide. Similar peptides were examined by high-voltage paper electrophoresis and detected by Fluorescamine. Peptides of low solubility have been examined in the presence of urea. The method reported here was used to resolve small peptides differing in size by a single amino acid, as well as peptides of the same size differing only by a sinle charge. It was successfully employed as a criterion for homogeneity of peptides in the course of purification for amino acid sequence analysis.",
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Peptides : An analytical method for their resolution by polyacrylamide gel electrophoresis applicable to a wide range of sizes and solubilities. / Rosemblatt, Mario S.; Margolies, Michael N.; Cannon, L. Edward; Haber, Edgar.

En: Analytical Biochemistry, Vol. 65, N.º 1-2, 12.05.1975, p. 321-330.

Resultado de la investigación: Article

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AU - Haber, Edgar

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