TY - JOUR
T1 - Peptide multifunctionalized gold nanorods decrease toxicity of β-amyloid peptide in a Caenorhabditis elegans model of Alzheimer's disease
AU - Morales-Zavala, Francisco
AU - Arriagada, Hector
AU - Hassan, Natalia
AU - Velasco, Carolina
AU - Riveros, Ana
AU - Álvarez, Alejandra R.
AU - Minniti, Alicia N.
AU - Rojas-Silva, Ximena
AU - Muñoz, Luis L.
AU - Vasquez, Rodrigo
AU - Rodriguez, Katherine
AU - Sanchez-Navarro, Macarena
AU - Giralt, Ernest
AU - Araya, Eyleen
AU - Aldunate, Rebeca
AU - Kogan, Marcelo J.
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The properties of nanometric materials make nanotechnology a promising platform for tackling problems of contemporary medicine. In this work, gold nanorods were synthetized and stabilized with polyethylene glycols and modified with two kinds of peptides. The D1 peptide that recognizes toxic aggregates of Aβ, a peptide involved in Alzheimer's disease (AD); and the Angiopep 2 that can be used to deliver nanorods to the mammalian central nervous system. The nanoconjugates were characterized using absorption spectrophotometry, dynamic light scattering, and transmission electron microscopy, among other techniques. We determined that the nanoconjugate does not affect neuronal viability; it penetrates the cells, and decreases aggregation of Aβ peptide in vitro. We also showed that when we apply our nanosystem to a Caenorhabditis elegans AD model, the toxicity of aggregated Aβ peptide is decreased. This work may contribute to the development of therapies for AD based on metallic nanoparticles.
AB - The properties of nanometric materials make nanotechnology a promising platform for tackling problems of contemporary medicine. In this work, gold nanorods were synthetized and stabilized with polyethylene glycols and modified with two kinds of peptides. The D1 peptide that recognizes toxic aggregates of Aβ, a peptide involved in Alzheimer's disease (AD); and the Angiopep 2 that can be used to deliver nanorods to the mammalian central nervous system. The nanoconjugates were characterized using absorption spectrophotometry, dynamic light scattering, and transmission electron microscopy, among other techniques. We determined that the nanoconjugate does not affect neuronal viability; it penetrates the cells, and decreases aggregation of Aβ peptide in vitro. We also showed that when we apply our nanosystem to a Caenorhabditis elegans AD model, the toxicity of aggregated Aβ peptide is decreased. This work may contribute to the development of therapies for AD based on metallic nanoparticles.
KW - Alzheimer's nanotherapy
KW - Amyloid-beta-peptide
KW - Drug-delivery
KW - Gold-nanoparticle
KW - Gold-nanorods
UR - http://www.scopus.com/inward/record.url?scp=85026900002&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2017.06.013
DO - 10.1016/j.nano.2017.06.013
M3 - Article
AN - SCOPUS:85026900002
SN - 1549-9634
VL - 13
SP - 2341
EP - 2350
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 7
ER -