Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase: Heterologous expression and characterization of the enzyme

Carolina Faúndez, Rodrigo Pérez, María Cristina Ravanal, J. Eyzaguirre

Resultado de la investigación: Contribución a una revistaArtículo

1 Cita (Scopus)

Resumen

Xylan, a component of plant cell walls, is composed of a backbone of β-1,4-linked xylopyranosyl units with a number of substituents. The complete degradation of xylan requires the action of several enzymes, among them β-xylosidase. The fungus Penicillium purpurogenum secretes a number of enzymes participating in the degradation of xylan. In this study, a β-xylosidase from this fungus was expressed in Pichia pastoris, and characterized. This enzyme (Xyl2) is a member of glycoside hydrolase family 3; it consists of a sequence of 792 residues including a signal peptide of 20 residues, with a theoretical molecular mass for the mature protein of 84.2 KDa and an isoelectric point of 5.07. The highest identity with a characterized fungal enzyme, is with a β-xylosidase from Aspergillus oryzae (70%). The optimal activity of Xyl2 is found at pH 2.0 and 28 °C. The enzyme is most stable at pH 2.0 and conserves 40% of activity at 42 °C (after 1h incubation). The kinetic parameters for p-nitrophenyl-β-D-xylopyranoside are: KM 0.53 mM, kcat 1*107 s−1 and kcat/KM 1.9*1010 M−1 s−1. The enzyme is about 10% active on p-nitrophenyl-α-L-arabinofuranoside. Xyl2 exhibits a high hydrolytic activity on xylooligosaccharides; it liberates xylose from beechwood and birchwood glucuronoxylan and it acts synergistically with endoxylanases in the degradation of xylan. Its low pH optimum make this enzyme particularly useful in potential applications requiring a low pH such as increasing the flavor of wine.

Idioma originalInglés
Número de artículo107738
PublicaciónCarbohydrate Research
Volumen482
DOI
EstadoPublicada - 1 ago 2019

Áreas temáticas de ASJC Scopus

  • Química analítica
  • Bioquímica
  • Química orgánica

Huella Profundice en los temas de investigación de 'Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase: Heterologous expression and characterization of the enzyme'. En conjunto forman una huella única.

  • Citar esto