Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase: Heterologous expression and characterization of the enzyme

Carolina Faúndez, Rodrigo Pérez, María Cristina Ravanal, J. Eyzaguirre

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Xylan, a component of plant cell walls, is composed of a backbone of β-1,4-linked xylopyranosyl units with a number of substituents. The complete degradation of xylan requires the action of several enzymes, among them β-xylosidase. The fungus Penicillium purpurogenum secretes a number of enzymes participating in the degradation of xylan. In this study, a β-xylosidase from this fungus was expressed in Pichia pastoris, and characterized. This enzyme (Xyl2) is a member of glycoside hydrolase family 3; it consists of a sequence of 792 residues including a signal peptide of 20 residues, with a theoretical molecular mass for the mature protein of 84.2 KDa and an isoelectric point of 5.07. The highest identity with a characterized fungal enzyme, is with a β-xylosidase from Aspergillus oryzae (70%). The optimal activity of Xyl2 is found at pH 2.0 and 28 °C. The enzyme is most stable at pH 2.0 and conserves 40% of activity at 42 °C (after 1h incubation). The kinetic parameters for p-nitrophenyl-β-D-xylopyranoside are: KM 0.53 mM, kcat 1*107 s−1 and kcat/KM 1.9*1010 M−1 s−1. The enzyme is about 10% active on p-nitrophenyl-α-L-arabinofuranoside. Xyl2 exhibits a high hydrolytic activity on xylooligosaccharides; it liberates xylose from beechwood and birchwood glucuronoxylan and it acts synergistically with endoxylanases in the degradation of xylan. Its low pH optimum make this enzyme particularly useful in potential applications requiring a low pH such as increasing the flavor of wine.

Idioma originalEnglish
Número de artículo107738
PublicaciónCarbohydrate Research
Volumen482
DOI
EstadoPublished - 1 ago 2019

Huella dactilar

Penicillium
Xylans
Xylosidases
Enzymes
Fungi
Degradation
Endo-1,4-beta Xylanases
Aspergillus oryzae
Wine
Pichia
Flavors
Glycoside Hydrolases
Aspergillus
Xylose
Isoelectric Point
Plant Cells
Molecular mass
Protein Sorting Signals
exo-1,4-beta-D-xylosidase
Kinetic parameters

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Citar esto

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title = "Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase: Heterologous expression and characterization of the enzyme",
abstract = "Xylan, a component of plant cell walls, is composed of a backbone of β-1,4-linked xylopyranosyl units with a number of substituents. The complete degradation of xylan requires the action of several enzymes, among them β-xylosidase. The fungus Penicillium purpurogenum secretes a number of enzymes participating in the degradation of xylan. In this study, a β-xylosidase from this fungus was expressed in Pichia pastoris, and characterized. This enzyme (Xyl2) is a member of glycoside hydrolase family 3; it consists of a sequence of 792 residues including a signal peptide of 20 residues, with a theoretical molecular mass for the mature protein of 84.2 KDa and an isoelectric point of 5.07. The highest identity with a characterized fungal enzyme, is with a β-xylosidase from Aspergillus oryzae (70{\%}). The optimal activity of Xyl2 is found at pH 2.0 and 28 °C. The enzyme is most stable at pH 2.0 and conserves 40{\%} of activity at 42 °C (after 1h incubation). The kinetic parameters for p-nitrophenyl-β-D-xylopyranoside are: KM 0.53 mM, kcat 1*107 s−1 and kcat/KM 1.9*1010 M−1 s−1. The enzyme is about 10{\%} active on p-nitrophenyl-α-L-arabinofuranoside. Xyl2 exhibits a high hydrolytic activity on xylooligosaccharides; it liberates xylose from beechwood and birchwood glucuronoxylan and it acts synergistically with endoxylanases in the degradation of xylan. Its low pH optimum make this enzyme particularly useful in potential applications requiring a low pH such as increasing the flavor of wine.",
keywords = "Heterologous expression, Lignocellulose biodegradation, Penicillium purpurogenum, Pichia pastoris, β-Xylosidase",
author = "Carolina Fa{\'u}ndez and Rodrigo P{\'e}rez and Ravanal, {Mar{\'i}a Cristina} and J. Eyzaguirre",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.carres.2019.06.017",
language = "English",
volume = "482",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier BV",

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Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase : Heterologous expression and characterization of the enzyme. / Faúndez, Carolina; Pérez, Rodrigo; Ravanal, María Cristina; Eyzaguirre, J.

En: Carbohydrate Research, Vol. 482, 107738, 01.08.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Penicillium purpurogenum produces a novel, acidic, GH3 beta-xylosidase

T2 - Heterologous expression and characterization of the enzyme

AU - Faúndez, Carolina

AU - Pérez, Rodrigo

AU - Ravanal, María Cristina

AU - Eyzaguirre, J.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Xylan, a component of plant cell walls, is composed of a backbone of β-1,4-linked xylopyranosyl units with a number of substituents. The complete degradation of xylan requires the action of several enzymes, among them β-xylosidase. The fungus Penicillium purpurogenum secretes a number of enzymes participating in the degradation of xylan. In this study, a β-xylosidase from this fungus was expressed in Pichia pastoris, and characterized. This enzyme (Xyl2) is a member of glycoside hydrolase family 3; it consists of a sequence of 792 residues including a signal peptide of 20 residues, with a theoretical molecular mass for the mature protein of 84.2 KDa and an isoelectric point of 5.07. The highest identity with a characterized fungal enzyme, is with a β-xylosidase from Aspergillus oryzae (70%). The optimal activity of Xyl2 is found at pH 2.0 and 28 °C. The enzyme is most stable at pH 2.0 and conserves 40% of activity at 42 °C (after 1h incubation). The kinetic parameters for p-nitrophenyl-β-D-xylopyranoside are: KM 0.53 mM, kcat 1*107 s−1 and kcat/KM 1.9*1010 M−1 s−1. The enzyme is about 10% active on p-nitrophenyl-α-L-arabinofuranoside. Xyl2 exhibits a high hydrolytic activity on xylooligosaccharides; it liberates xylose from beechwood and birchwood glucuronoxylan and it acts synergistically with endoxylanases in the degradation of xylan. Its low pH optimum make this enzyme particularly useful in potential applications requiring a low pH such as increasing the flavor of wine.

AB - Xylan, a component of plant cell walls, is composed of a backbone of β-1,4-linked xylopyranosyl units with a number of substituents. The complete degradation of xylan requires the action of several enzymes, among them β-xylosidase. The fungus Penicillium purpurogenum secretes a number of enzymes participating in the degradation of xylan. In this study, a β-xylosidase from this fungus was expressed in Pichia pastoris, and characterized. This enzyme (Xyl2) is a member of glycoside hydrolase family 3; it consists of a sequence of 792 residues including a signal peptide of 20 residues, with a theoretical molecular mass for the mature protein of 84.2 KDa and an isoelectric point of 5.07. The highest identity with a characterized fungal enzyme, is with a β-xylosidase from Aspergillus oryzae (70%). The optimal activity of Xyl2 is found at pH 2.0 and 28 °C. The enzyme is most stable at pH 2.0 and conserves 40% of activity at 42 °C (after 1h incubation). The kinetic parameters for p-nitrophenyl-β-D-xylopyranoside are: KM 0.53 mM, kcat 1*107 s−1 and kcat/KM 1.9*1010 M−1 s−1. The enzyme is about 10% active on p-nitrophenyl-α-L-arabinofuranoside. Xyl2 exhibits a high hydrolytic activity on xylooligosaccharides; it liberates xylose from beechwood and birchwood glucuronoxylan and it acts synergistically with endoxylanases in the degradation of xylan. Its low pH optimum make this enzyme particularly useful in potential applications requiring a low pH such as increasing the flavor of wine.

KW - Heterologous expression

KW - Lignocellulose biodegradation

KW - Penicillium purpurogenum

KW - Pichia pastoris

KW - β-Xylosidase

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DO - 10.1016/j.carres.2019.06.017

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