Penicillium purpurogenum produces a highly stable endo-β-(1,4)-galactanase

Vasni Zavaleta, Jaime Eyzaguirre

Resultado de la investigación: Contribución a una revistaArtículo

3 Citas (Scopus)

Resumen

The polysaccharides of galactose present in the pectin of the plant cell wall are degraded by endo-β-1,4-galactanases. The filamentous fungus Penicillium purpurogenum, which grows on a number of natural carbon sources, among them sugar beet pulp which contains pectin, has a gene (ppgal1) coding an endo-β-1,4-galactanase (PpGAL1). This enzyme was expressed heterologously in Pichia pastoris. It has a molecular mass of 38 kDa, a pH optimum of 4–4.5, and an optimal temperature of 60 °C. It is 100 % stable for up to 24 h at pH 4–4.5 and 40 °C. These stability properties, which exceed those from other endo-β-1,4-galactanases reported to date, make it particularly suitable for industrial processes requiring acidic conditions and temperatures up to 40 °C. PpGAL1 is, therefore, a potentially effective tool in the food industry and in other biotechnological applications.

Idioma originalInglés
Páginas (desde-hasta)1313-1327
Número de páginas15
PublicaciónApplied Biochemistry and Biotechnology
Volumen180
N.º7
DOI
EstadoPublicada - 1 dic 2016

Áreas temáticas de ASJC Scopus

  • Biotecnología
  • Bioingeniería
  • Bioquímica
  • Microbiología y biotecnología aplicadas
  • Biología molecular

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