Penicillium purpurogenum produces a highly stable endo-β-(1,4)-galactanase

Vasni Zavaleta, Jaime Eyzaguirre

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

The polysaccharides of galactose present in the pectin of the plant cell wall are degraded by endo-β-1,4-galactanases. The filamentous fungus Penicillium purpurogenum, which grows on a number of natural carbon sources, among them sugar beet pulp which contains pectin, has a gene (ppgal1) coding an endo-β-1,4-galactanase (PpGAL1). This enzyme was expressed heterologously in Pichia pastoris. It has a molecular mass of 38 kDa, a pH optimum of 4–4.5, and an optimal temperature of 60 °C. It is 100 % stable for up to 24 h at pH 4–4.5 and 40 °C. These stability properties, which exceed those from other endo-β-1,4-galactanases reported to date, make it particularly suitable for industrial processes requiring acidic conditions and temperatures up to 40 °C. PpGAL1 is, therefore, a potentially effective tool in the food industry and in other biotechnological applications.

Idioma originalEnglish
Páginas (desde-hasta)1313-1327
Número de páginas15
PublicaciónApplied Biochemistry and Biotechnology
Volumen180
N.º7
DOI
EstadoPublished - 1 dic 2016

Huella dactilar

Penicillium
Sugar beets
Temperature
Beta vulgaris
Pichia
Food Industry
Plant Cells
Molecular mass
Polysaccharides
Fungi
Galactose
Cell Wall
Pulp
Carbon
Enzymes
Genes
Industry
pectin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Citar esto

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abstract = "The polysaccharides of galactose present in the pectin of the plant cell wall are degraded by endo-β-1,4-galactanases. The filamentous fungus Penicillium purpurogenum, which grows on a number of natural carbon sources, among them sugar beet pulp which contains pectin, has a gene (ppgal1) coding an endo-β-1,4-galactanase (PpGAL1). This enzyme was expressed heterologously in Pichia pastoris. It has a molecular mass of 38 kDa, a pH optimum of 4–4.5, and an optimal temperature of 60 °C. It is 100 {\%} stable for up to 24 h at pH 4–4.5 and 40 °C. These stability properties, which exceed those from other endo-β-1,4-galactanases reported to date, make it particularly suitable for industrial processes requiring acidic conditions and temperatures up to 40 °C. PpGAL1 is, therefore, a potentially effective tool in the food industry and in other biotechnological applications.",
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Penicillium purpurogenum produces a highly stable endo-β-(1,4)-galactanase. / Zavaleta, Vasni; Eyzaguirre, Jaime.

En: Applied Biochemistry and Biotechnology, Vol. 180, N.º 7, 01.12.2016, p. 1313-1327.

Resultado de la investigación: Article

TY - JOUR

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N2 - The polysaccharides of galactose present in the pectin of the plant cell wall are degraded by endo-β-1,4-galactanases. The filamentous fungus Penicillium purpurogenum, which grows on a number of natural carbon sources, among them sugar beet pulp which contains pectin, has a gene (ppgal1) coding an endo-β-1,4-galactanase (PpGAL1). This enzyme was expressed heterologously in Pichia pastoris. It has a molecular mass of 38 kDa, a pH optimum of 4–4.5, and an optimal temperature of 60 °C. It is 100 % stable for up to 24 h at pH 4–4.5 and 40 °C. These stability properties, which exceed those from other endo-β-1,4-galactanases reported to date, make it particularly suitable for industrial processes requiring acidic conditions and temperatures up to 40 °C. PpGAL1 is, therefore, a potentially effective tool in the food industry and in other biotechnological applications.

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