Penicillium purpurogenum produces a family 1 acetyl xylan esterase containing a carbohydrate-binding module: characterization of the protein and its gene

Felipe Gordillo, Valentina Caputo, Alessandra Peirano, Renato Chavez, Jozef Van Beeumen, Isabel Vandenberghe, Marc Claeyssens, Paulina Bull, María Cristina Ravanal, Jaime Eyzaguirre

Resultado de la investigación: Contribución a una revistaArtículo

19 Citas (Scopus)

Resumen

At least three acetyl xylan esterases (AXE I, II and III) are secreted by Penicillium purpurogenum. This publication describes more detailed work on AXE I and its gene. AXE I binds cellulose but not xylan; it is glycosylated and inactivated by phenylmethylsulphonyl fluoride, showing that it is a serine esterase. The axe1 gene presents an open reading frame of 1278 bp, including two introns of 68 and 61 bp; it codes for a signal peptide of 31 residues and a mature protein of 351 amino acids (molecular weight 36,693). AXE I has a modular structure: a catalytic module at the amino terminus belonging to family 1 of the carbohydrate esterases, a linker rich in serines and threonines, and a family 1 carboxy terminal carbohydrate binding module (CBM). The CBM is similar to that of AXE from Trichoderma reesei, (with a family 5 catalytic module) indicating that the genes for catalytic modules and CBMs have evolved separately, and that they have been linked by gene fusion. The promoter sequence of axe1 contains several putative sequences for binding of gene expression regulators also found in other family 1 esterase gene promoters. It is proposed that AXE I and II act in succession in xylan degradation; first, xylan is attacked by AXE I and other xylanases possessing CBMs (which facilitate binding to lignocellulose), followed by other enzymes acting mainly on soluble substrates.

Idioma originalInglés
Páginas (desde-hasta)1129-1139
Número de páginas11
PublicaciónMycological Research
Volumen110
N.º10
DOI
EstadoPublicada - 1 oct 2006

Áreas temáticas de ASJC Scopus

  • Agricultura y biología (miscelánea)
  • Botánica

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    Gordillo, F., Caputo, V., Peirano, A., Chavez, R., Van Beeumen, J., Vandenberghe, I., Claeyssens, M., Bull, P., Ravanal, M. C., & Eyzaguirre, J. (2006). Penicillium purpurogenum produces a family 1 acetyl xylan esterase containing a carbohydrate-binding module: characterization of the protein and its gene. Mycological Research, 110(10), 1129-1139. https://doi.org/10.1016/j.mycres.2006.07.003