Pathoscope: Species identification and strain attribution with unassembled sequencing data

Owen E. Francis, Matthew Bendall, Solaiappan Manimaran, Changjin Hong, Nathan L. Clement, Eduardo Castro-Nallar, Quinn Snell, G. Bruce Schaalje, Mark J. Clement, Keith A. Crandall, W. Evan Johnson

Resultado de la investigación: Contribución a una revistaArtículo

72 Citas (Scopus)

Resumen

Emerging next-generation sequencing technologies have revolutionized the collection of genomic data for applications in bioforensics, biosurveillance, and for use in clinical settings. However, to make the most of these new data, new methodology needs to be developed that can accommodate large volumes of genetic data in a computationally efficient manner. We present a statistical framework to analyze raw next-generation sequence reads from purified or mixed environmental or targeted infected tissue samples for rapid species identification and strain attribution against a robust database of known biological agents. Our method, Pathoscope, capitalizes on a Bayesian statistical framework that accommodates information on sequence quality, mapping quality, and provides posterior probabilities of matches to a known database of target genomes. Importantly, our approach also incorporates the possibility that multiple species can be present in the sample and considers cases when the sample species/strain is not in the reference database. Furthermore, our approach can accurately discriminate between very closely related strains of the same species with very little coverage of the genome and without the need for multiple alignment steps, extensive homology searches, or genome assembly - which are time-consuming and labor-intensive steps. We demonstrate the utility of our approach on genomic data from purified and in silico ''environmental'' samples from known bacterial agents impacting human health for accuracy assessment and comparison with other approaches.

Idioma originalInglés
Páginas (desde-hasta)1721-1729
Número de páginas9
PublicaciónGenome Research
Volumen23
N.º10
DOI
EstadoPublicada - oct 2013

Áreas temáticas de ASJC Scopus

  • Genética
  • Genética (clínica)

Huella Profundice en los temas de investigación de 'Pathoscope: Species identification and strain attribution with unassembled sequencing data'. En conjunto forman una huella única.

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    Francis, O. E., Bendall, M., Manimaran, S., Hong, C., Clement, N. L., Castro-Nallar, E., Snell, Q., Schaalje, G. B., Clement, M. J., Crandall, K. A., & Johnson, W. E. (2013). Pathoscope: Species identification and strain attribution with unassembled sequencing data. Genome Research, 23(10), 1721-1729. https://doi.org/10.1101/gr.150151.112