Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice

Francisco Ipinza, Bernardo Collao, Debbie Monsalva, Victor H. Bustamante, Roberto Luraschi, Melissa Alegría-Arcos, Daniel E. Almonacid, Daniel Aguayo, Iván L. Calderón, Fernando Gil, Carlos A. Santiviago, Eduardo H. Morales, Edmundo Calva, Claudia P. Saavedra

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.

Idioma originalEnglish
Número de artículoe111062
PublicaciónPLoS ONE
Volumen9
N.º10
DOI
EstadoPublished - 31 oct 2014

Huella dactilar

porins
Porins
Salmonella
Macrophages
macrophages
mice
Infection
infection
Salmonella typhimurium
Salmonella Typhimurium
reactive oxygen species
Reactive Oxygen Species
Down-Regulation
Typhoid Fever
etiological agents
gastroenteritis
Gastroenteritis
fever
animal models
Phenotype

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Ipinza, F., Collao, B., Monsalva, D., Bustamante, V. H., Luraschi, R., Alegría-Arcos, M., ... Saavedra, C. P. (2014). Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice. PLoS ONE, 9(10), [e111062]. https://doi.org/10.1371/journal.pone.0111062
Ipinza, Francisco ; Collao, Bernardo ; Monsalva, Debbie ; Bustamante, Victor H. ; Luraschi, Roberto ; Alegría-Arcos, Melissa ; Almonacid, Daniel E. ; Aguayo, Daniel ; Calderón, Iván L. ; Gil, Fernando ; Santiviago, Carlos A. ; Morales, Eduardo H. ; Calva, Edmundo ; Saavedra, Claudia P. / Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice. En: PLoS ONE. 2014 ; Vol. 9, N.º 10.
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abstract = "Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.",
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Ipinza, F, Collao, B, Monsalva, D, Bustamante, VH, Luraschi, R, Alegría-Arcos, M, Almonacid, DE, Aguayo, D, Calderón, IL, Gil, F, Santiviago, CA, Morales, EH, Calva, E & Saavedra, CP 2014, 'Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice', PLoS ONE, vol. 9, n.º 10, e111062. https://doi.org/10.1371/journal.pone.0111062

Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice. / Ipinza, Francisco; Collao, Bernardo; Monsalva, Debbie; Bustamante, Victor H.; Luraschi, Roberto; Alegría-Arcos, Melissa; Almonacid, Daniel E.; Aguayo, Daniel; Calderón, Iván L.; Gil, Fernando; Santiviago, Carlos A.; Morales, Eduardo H.; Calva, Edmundo; Saavedra, Claudia P.

En: PLoS ONE, Vol. 9, N.º 10, e111062, 31.10.2014.

Resultado de la investigación: Article

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T1 - Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice

AU - Ipinza, Francisco

AU - Collao, Bernardo

AU - Monsalva, Debbie

AU - Bustamante, Victor H.

AU - Luraschi, Roberto

AU - Alegría-Arcos, Melissa

AU - Almonacid, Daniel E.

AU - Aguayo, Daniel

AU - Calderón, Iván L.

AU - Gil, Fernando

AU - Santiviago, Carlos A.

AU - Morales, Eduardo H.

AU - Calva, Edmundo

AU - Saavedra, Claudia P.

PY - 2014/10/31

Y1 - 2014/10/31

N2 - Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.

AB - Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever in mice. Inside these hosts, Salmonella must overcome hostile conditions to develop a successful infection, a process in which the levels of porins may be critical. Herein, the role of the Salmonella Typhimurium porin OmpD in the infection process was assessed for adherence, invasion and proliferation in RAW264.7 mouse macrophages and in BALB/c mice. In cultured macrophages, a ΔompD strain exhibited increased invasion and proliferation phenotypes as compared to its parental strain. In contrast, overexpression of ompD caused a reduction in bacterial proliferation but did not affect adherence or invasion. In the murine model, the ΔompD strain showed increased ability to survive and replicate in target organs of infection. The ompD transcript levels showed a down-regulation when Salmonella resided within cultured macrophages and when it colonized target organs in infected mice. Additionally, cultured macrophages infected with the ΔompD strain produced lower levels of reactive oxygen species, suggesting that down-regulation of ompD could favor replication of Salmonella inside macrophages and the subsequent systemic dissemination, by limiting the reactive oxygen species response of the host.

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DO - 10.1371/journal.pone.0111062

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Ipinza F, Collao B, Monsalva D, Bustamante VH, Luraschi R, Alegría-Arcos M y otros. Participation of the Salmonella OmpD porin in the infection of RAW264.7 macrophages and BALB/c mice. PLoS ONE. 2014 oct 31;9(10). e111062. https://doi.org/10.1371/journal.pone.0111062