Participation of S. Typhimurium cysJIH operon in the H2S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source

Ricardo Álvarez; Jorge Frávega; Paula I. Rodas; Juan A. Fuentes; Daniel Paredes-Sabja; Iván L. Calderón; Fernando Gil

doi:10.3390/antibiotics4030321

Participation of S. Typhimurium _cysJIH operon in the H₂S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source

Ricardo Álvarez, Jorge Frávega, Paula I. Rodas, Juan A. Fuentes, Daniel Paredes-Sabja, Iván L. Calderón, Fernando Gil

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

3 Citas (Scopus)

Resumen

H₂S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H₂S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H₂S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.

Idioma original	Inglés
Páginas (desde-hasta)	321-328
Número de páginas	8
Publicación	Antibiotics
Volumen	4
N.º	3
DOI	https://doi.org/10.3390/antibiotics4030321
Estado	Publicada - 31 jul. 2015

Áreas temáticas de ASJC Scopus

Microbiología
Bioquímica
Farmacología, toxicología y ciencias farmacéuticas (todo)
Microbiología (médica)
Enfermedades infecciosas
Farmacología (médica)

ODS de las Naciones Unidas

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abstract = "H2S production has been proposed as a mechanism to explain bacterial resistance to antibiotics. In this work, we present evidence for the role of the cysJIH operon in resistance to ciprofloxacin mediated by H2S production with different sulfate as the only sulfur source. We found that the products of the cysJIH operon are involved in ciprofloxacin resistance by increasing both, the levels of H2S and reduced thiols apparently counteracting antimicrobial-induced reactive oxygen species (ROS). This protective effect was observed only when bacteria were cultured in the presence of sulfate, but not with cysteine, as the sole sulfur source.",

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AU - Rodas, Paula I.

AU - Fuentes, Juan A.

AU - Paredes-Sabja, Daniel

AU - Calderón, Iván L.

AU - Gil, Fernando

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