Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic

Peter Kämpfer, Rute Irgang, Matías Poblete-Morales, Guillermo Fernández-Negrete, Stefanie P. Glaeser, Derie Fuentes-Messina, Ruben Avendaño-Herrera

Resultado de la investigación: Article

Resumen

An orange-pigmented, oxidase-positive bacterial strain (I-41R45T), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45T with sequences of type strains of most closely related Paracoccus species showed highest sequence similarities to Paracoccus hibiscisoli (98.4 %), Paracoccus marcusii (98.3 %), Paracoccus haeundaensis and Paracoccus carotinifaciens (both 98.2 %). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97 %. The draft genome of strain I-41R45T had a size of 4.59 Mb with a DNA G+C content of 65.26 mol% and included the prediction and annotation of 4426 coding genes, 1973 protein-coding genes and 46 tRNAs. The fatty acid profile of strain I-41R45T consisted mainly of the major fatty acids C18 : 1ω7c/ω9t/ω12t and C18:0, typical of the genus Paracoccus. DNA-DNA hybridizations between I-41R45T and type strains of P. hibiscisoli, P. marcusiiand P. haeundaensis resulted in similarity values of 45 % (reciprocal 26 %), 66 % (reciprocal 61 %), and 29 % (reciprocal 36 %), respectively. DNA-DNA hybridization results, together with the differentiating biochemical and chemotaxonomic properties, showed that strain I-41R45T represents a novel Paracoccus species, for which the name Paracoccus nototheniae sp. nov. (type strain I-41R45T=CCM 8875T=CIP 111632T), is proposed.

Idioma originalEnglish
Páginas (desde-hasta)2794-2800
Número de páginas7
PublicaciónInternational Journal of Systematic and Evolutionary Microbiology
Volumen69
N.º9
DOI
EstadoPublished - 1 sep 2019

Huella dactilar

Paracoccus (Proteobacteria)
Paracoccus
Gadiformes
cod (fish)
Fishes
rocks
DNA
new species
fish
rock
gene
fatty acid
Paracoccus carotinifaciens
nucleic acid hybridization
Paracoccus marcusii
rRNA Genes
ribosomal RNA
Fatty Acids
nucleotide sequences
genome

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Citar esto

Kämpfer, Peter ; Irgang, Rute ; Poblete-Morales, Matías ; Fernández-Negrete, Guillermo ; Glaeser, Stefanie P. ; Fuentes-Messina, Derie ; Avendaño-Herrera, Ruben. / Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic. En: International Journal of Systematic and Evolutionary Microbiology. 2019 ; Vol. 69, N.º 9. pp. 2794-2800.
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title = "Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic",
abstract = "An orange-pigmented, oxidase-positive bacterial strain (I-41R45T), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45T with sequences of type strains of most closely related Paracoccus species showed highest sequence similarities to Paracoccus hibiscisoli (98.4 {\%}), Paracoccus marcusii (98.3 {\%}), Paracoccus haeundaensis and Paracoccus carotinifaciens (both 98.2 {\%}). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97 {\%}. The draft genome of strain I-41R45T had a size of 4.59 Mb with a DNA G+C content of 65.26 mol{\%} and included the prediction and annotation of 4426 coding genes, 1973 protein-coding genes and 46 tRNAs. The fatty acid profile of strain I-41R45T consisted mainly of the major fatty acids C18 : 1ω7c/ω9t/ω12t and C18:0, typical of the genus Paracoccus. DNA-DNA hybridizations between I-41R45T and type strains of P. hibiscisoli, P. marcusiiand P. haeundaensis resulted in similarity values of 45 {\%} (reciprocal 26 {\%}), 66 {\%} (reciprocal 61 {\%}), and 29 {\%} (reciprocal 36 {\%}), respectively. DNA-DNA hybridization results, together with the differentiating biochemical and chemotaxonomic properties, showed that strain I-41R45T represents a novel Paracoccus species, for which the name Paracoccus nototheniae sp. nov. (type strain I-41R45T=CCM 8875T=CIP 111632T), is proposed.",
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Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic. / Kämpfer, Peter; Irgang, Rute; Poblete-Morales, Matías; Fernández-Negrete, Guillermo; Glaeser, Stefanie P.; Fuentes-Messina, Derie; Avendaño-Herrera, Ruben.

En: International Journal of Systematic and Evolutionary Microbiology, Vol. 69, N.º 9, 01.09.2019, p. 2794-2800.

Resultado de la investigación: Article

TY - JOUR

T1 - Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic

AU - Kämpfer, Peter

AU - Irgang, Rute

AU - Poblete-Morales, Matías

AU - Fernández-Negrete, Guillermo

AU - Glaeser, Stefanie P.

AU - Fuentes-Messina, Derie

AU - Avendaño-Herrera, Ruben

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N2 - An orange-pigmented, oxidase-positive bacterial strain (I-41R45T), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45T with sequences of type strains of most closely related Paracoccus species showed highest sequence similarities to Paracoccus hibiscisoli (98.4 %), Paracoccus marcusii (98.3 %), Paracoccus haeundaensis and Paracoccus carotinifaciens (both 98.2 %). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97 %. The draft genome of strain I-41R45T had a size of 4.59 Mb with a DNA G+C content of 65.26 mol% and included the prediction and annotation of 4426 coding genes, 1973 protein-coding genes and 46 tRNAs. The fatty acid profile of strain I-41R45T consisted mainly of the major fatty acids C18 : 1ω7c/ω9t/ω12t and C18:0, typical of the genus Paracoccus. DNA-DNA hybridizations between I-41R45T and type strains of P. hibiscisoli, P. marcusiiand P. haeundaensis resulted in similarity values of 45 % (reciprocal 26 %), 66 % (reciprocal 61 %), and 29 % (reciprocal 36 %), respectively. DNA-DNA hybridization results, together with the differentiating biochemical and chemotaxonomic properties, showed that strain I-41R45T represents a novel Paracoccus species, for which the name Paracoccus nototheniae sp. nov. (type strain I-41R45T=CCM 8875T=CIP 111632T), is proposed.

AB - An orange-pigmented, oxidase-positive bacterial strain (I-41R45T), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45T with sequences of type strains of most closely related Paracoccus species showed highest sequence similarities to Paracoccus hibiscisoli (98.4 %), Paracoccus marcusii (98.3 %), Paracoccus haeundaensis and Paracoccus carotinifaciens (both 98.2 %). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97 %. The draft genome of strain I-41R45T had a size of 4.59 Mb with a DNA G+C content of 65.26 mol% and included the prediction and annotation of 4426 coding genes, 1973 protein-coding genes and 46 tRNAs. The fatty acid profile of strain I-41R45T consisted mainly of the major fatty acids C18 : 1ω7c/ω9t/ω12t and C18:0, typical of the genus Paracoccus. DNA-DNA hybridizations between I-41R45T and type strains of P. hibiscisoli, P. marcusiiand P. haeundaensis resulted in similarity values of 45 % (reciprocal 26 %), 66 % (reciprocal 61 %), and 29 % (reciprocal 36 %), respectively. DNA-DNA hybridization results, together with the differentiating biochemical and chemotaxonomic properties, showed that strain I-41R45T represents a novel Paracoccus species, for which the name Paracoccus nototheniae sp. nov. (type strain I-41R45T=CCM 8875T=CIP 111632T), is proposed.

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