PAGE analysis of the heteroduplexes formed between PCR-amplified 16S rRNA genes: estimation of sequence similarity and rDNA complexity.

R. T. Espejo, C. G. Feijóo, J. Romero, M. Vásquez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Analysis of the 16S rRNA genes retrieved directly from different environments has proven to be a powerful tool that has greatly expanded our knowledge of microbial diversity and phylogeny. It is shown here that sequence similarity between 80 and 100% among 16S rDNAs can be estimated by the electrophoretic migration of their heteroduplexes. This was measured by hybridization and electrophoresis in polyacrylamide gels of the product obtained after PCR amplification of almost the entire 16S rRNA gene from different bacterial species. These heteroduplexes were also observed after amplification of samples containing DNA from two or more bacterial species and a procedure was applied to identify reliably heteroduplexes among the amplification products. The electrophoretic migration of the heteroduplexes observed after PCR was used to detect the presence of 16S rDNAs with different sequences in DNA extracted from both a mixture of two bacterial species and samples containing a natural bacterial community.

Idioma originalInglés
PublicaciónMicrobiology (Reading, England)
Volumen144
EstadoPublicada - jun 1998

Áreas temáticas de ASJC Scopus

  • Microbiología

Huella

Profundice en los temas de investigación de 'PAGE analysis of the heteroduplexes formed between PCR-amplified 16S rRNA genes: estimation of sequence similarity and rDNA complexity.'. En conjunto forman una huella única.

Citar esto