Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins

R. F. Castilho, A. R. Meinicke, A. M. Almeida, M. Hermes-Lima, A. E. Vercesi

Resultado de la investigación: Contribución a una revistaArtículo

73 Citas (Scopus)

Resumen

Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.

Idioma originalInglés
Páginas (desde-hasta)158-163
Número de páginas6
PublicaciónArchives of Biochemistry and Biophysics
Volumen308
N.º1
DOI
EstadoPublicada - 1994

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biofísica
  • Biología molecular

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