Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins

R. F. Castilho, A. R. Meinicke, A. M. Almeida, M. Hermes-Lima, A. E. Vercesi

Resultado de la investigación: Article

73 Citas (Scopus)

Resumen

Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.

Idioma originalEnglish
Páginas (desde-hasta)158-163
Número de páginas6
PublicaciónArchives of Biochemistry and Biophysics
Volumen308
N.º1
DOI
EstadoPublished - 1994

Huella dactilar

Mitochondria
Lipid Peroxidation
Membrane Proteins
Lipids
Dibucaine
Mitochondrial Swelling
Ruthenium Red
Schiff Bases
Thiobarbituric Acid Reactive Substances
Liver Mitochondrion
Egtazic Acid
Electrophoresis
Liver
Membrane Potentials
Swelling
Rats
Polyacrylamide Gel Electrophoresis
Membranes
monoferrous acid citrate
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Citar esto

Castilho, R. F. ; Meinicke, A. R. ; Almeida, A. M. ; Hermes-Lima, M. ; Vercesi, A. E. / Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins. En: Archives of Biochemistry and Biophysics. 1994 ; Vol. 308, N.º 1. pp. 158-163.
@article{e756284db6a146cc8429832b222e84b4,
title = "Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins",
abstract = "Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.",
author = "Castilho, {R. F.} and Meinicke, {A. R.} and Almeida, {A. M.} and M. Hermes-Lima and Vercesi, {A. E.}",
year = "1994",
doi = "10.1006/abbi.1994.1022",
language = "English",
volume = "308",
pages = "158--163",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins. / Castilho, R. F.; Meinicke, A. R.; Almeida, A. M.; Hermes-Lima, M.; Vercesi, A. E.

En: Archives of Biochemistry and Biophysics, Vol. 308, N.º 1, 1994, p. 158-163.

Resultado de la investigación: Article

TY - JOUR

T1 - Oxidative damage of mitochondria induced by Fe(II)citrate is potentiated by Ca2+ and includes lipid peroxidation and alterations in membrane proteins

AU - Castilho, R. F.

AU - Meinicke, A. R.

AU - Almeida, A. M.

AU - Hermes-Lima, M.

AU - Vercesi, A. E.

PY - 1994

Y1 - 1994

N2 - Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.

AB - Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.

UR - http://www.scopus.com/inward/record.url?scp=0028272219&partnerID=8YFLogxK

U2 - 10.1006/abbi.1994.1022

DO - 10.1006/abbi.1994.1022

M3 - Article

C2 - 7508707

AN - SCOPUS:0028272219

VL - 308

SP - 158

EP - 163

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -