Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes

Ángela Plaza-Garrido, Camila Miranda-Cárdenas, Pablo Castro-Córdova, Valeria Olguín-Araneda, Glenda Cofré-Araneda, Cristian Hernández-Rocha, Robert Carman, Patricio Ibáñez, Warren N. Fawley, Mark H. Wilcox, Fernando Gil, Iván L. Calderón, Juan A. Fuentes, Ana María Guzmán-Durán, Manuel Alvarez-Lobos, Daniel Paredes-Sabja

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

One of the main clinical challenges of Clostridium difficile infections (CDI) is the high rate of relapse episodes. The main determinants involved in relapse of CDI include the presence of antibiotic-resistant C. difficile spores in the colonic environment and a permanent state of dysbiosis of the microbiota caused by antibiotic therapy. A possible scenario is that phenotypes related to the persistence of C. difficile spores might contribute to relapsing infections. In this study, 8 C. difficile isolates recovered from 4 cases with relapsing infection, and 9 isolates recovered from single infection cases were analyzed for PCR ribotyping and the presence of tcdA, tcdB and cdtAB genes. Factors associated to spore persistence, sporulation, spore adherence and biofilm formation and sporulation during biofilm formation were characterized. We also evaluated motility and cytotoxicity. However, we observed no significant difference in the analyzed phenotypes among the different clinical outcomes, most likely due to the high variability observed among strains within clinical backgrounds in each phenotype and the small sample size. It is noteworthy that C. difficile spores adhered to similar extents to undifferentiated and differentiated Caco-2 cells. By contrast, spores of all clinical isolates tested had increased germination efficiency in presence of taurocholate, while decreased sporulation rate during biofilm development in the presence of glucose. In conclusion, these results show that, at least in this cohort of patients, the described phenotypes are not detrimental in the clinical outcome of the disease.

Idioma originalEnglish
Páginas (desde-hasta)30-38
Número de páginas9
PublicaciónAnaerobe
Volumen36
DOI
EstadoPublished - 1 dic 2015

Huella dactilar

Clostridium Infections
Clostridium difficile
Spores
Virulence
Phenotype
Biofilms
Infection
Dysbiosis
Anti-Bacterial Agents
Ribotyping
Recurrence
Taurocholic Acid
Caco-2 Cells
Microbiota
Germination
Sample Size
Glucose
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Citar esto

Plaza-Garrido, Á., Miranda-Cárdenas, C., Castro-Córdova, P., Olguín-Araneda, V., Cofré-Araneda, G., Hernández-Rocha, C., ... Paredes-Sabja, D. (2015). Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes. Anaerobe, 36, 30-38. https://doi.org/10.1016/j.anaerobe.2015.09.005
Plaza-Garrido, Ángela ; Miranda-Cárdenas, Camila ; Castro-Córdova, Pablo ; Olguín-Araneda, Valeria ; Cofré-Araneda, Glenda ; Hernández-Rocha, Cristian ; Carman, Robert ; Ibáñez, Patricio ; Fawley, Warren N. ; Wilcox, Mark H. ; Gil, Fernando ; Calderón, Iván L. ; Fuentes, Juan A. ; Guzmán-Durán, Ana María ; Alvarez-Lobos, Manuel ; Paredes-Sabja, Daniel. / Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes. En: Anaerobe. 2015 ; Vol. 36. pp. 30-38.
@article{1384724d7966440aab728b4a22ce32f8,
title = "Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes",
abstract = "One of the main clinical challenges of Clostridium difficile infections (CDI) is the high rate of relapse episodes. The main determinants involved in relapse of CDI include the presence of antibiotic-resistant C. difficile spores in the colonic environment and a permanent state of dysbiosis of the microbiota caused by antibiotic therapy. A possible scenario is that phenotypes related to the persistence of C. difficile spores might contribute to relapsing infections. In this study, 8 C. difficile isolates recovered from 4 cases with relapsing infection, and 9 isolates recovered from single infection cases were analyzed for PCR ribotyping and the presence of tcdA, tcdB and cdtAB genes. Factors associated to spore persistence, sporulation, spore adherence and biofilm formation and sporulation during biofilm formation were characterized. We also evaluated motility and cytotoxicity. However, we observed no significant difference in the analyzed phenotypes among the different clinical outcomes, most likely due to the high variability observed among strains within clinical backgrounds in each phenotype and the small sample size. It is noteworthy that C. difficile spores adhered to similar extents to undifferentiated and differentiated Caco-2 cells. By contrast, spores of all clinical isolates tested had increased germination efficiency in presence of taurocholate, while decreased sporulation rate during biofilm development in the presence of glucose. In conclusion, these results show that, at least in this cohort of patients, the described phenotypes are not detrimental in the clinical outcome of the disease.",
keywords = "Biofilm, Clostridium difficile, Recurrent CDI, Relapse, Relapsing CDI, Spore adherence, Spore germination, Sporulation",
author = "{\'A}ngela Plaza-Garrido and Camila Miranda-C{\'a}rdenas and Pablo Castro-C{\'o}rdova and Valeria Olgu{\'i}n-Araneda and Glenda Cofr{\'e}-Araneda and Cristian Hern{\'a}ndez-Rocha and Robert Carman and Patricio Ib{\'a}{\~n}ez and Fawley, {Warren N.} and Wilcox, {Mark H.} and Fernando Gil and Calder{\'o}n, {Iv{\'a}n L.} and Fuentes, {Juan A.} and Guzm{\'a}n-Dur{\'a}n, {Ana Mar{\'i}a} and Manuel Alvarez-Lobos and Daniel Paredes-Sabja",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.anaerobe.2015.09.005",
language = "English",
volume = "36",
pages = "30--38",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press Inc.",

}

Plaza-Garrido, Á, Miranda-Cárdenas, C, Castro-Córdova, P, Olguín-Araneda, V, Cofré-Araneda, G, Hernández-Rocha, C, Carman, R, Ibáñez, P, Fawley, WN, Wilcox, MH, Gil, F, Calderón, IL, Fuentes, JA, Guzmán-Durán, AM, Alvarez-Lobos, M & Paredes-Sabja, D 2015, 'Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes', Anaerobe, vol. 36, pp. 30-38. https://doi.org/10.1016/j.anaerobe.2015.09.005

Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes. / Plaza-Garrido, Ángela; Miranda-Cárdenas, Camila; Castro-Córdova, Pablo; Olguín-Araneda, Valeria; Cofré-Araneda, Glenda; Hernández-Rocha, Cristian; Carman, Robert; Ibáñez, Patricio; Fawley, Warren N.; Wilcox, Mark H.; Gil, Fernando; Calderón, Iván L.; Fuentes, Juan A.; Guzmán-Durán, Ana María; Alvarez-Lobos, Manuel; Paredes-Sabja, Daniel.

En: Anaerobe, Vol. 36, 01.12.2015, p. 30-38.

Resultado de la investigación: Article

TY - JOUR

T1 - Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes

AU - Plaza-Garrido, Ángela

AU - Miranda-Cárdenas, Camila

AU - Castro-Córdova, Pablo

AU - Olguín-Araneda, Valeria

AU - Cofré-Araneda, Glenda

AU - Hernández-Rocha, Cristian

AU - Carman, Robert

AU - Ibáñez, Patricio

AU - Fawley, Warren N.

AU - Wilcox, Mark H.

AU - Gil, Fernando

AU - Calderón, Iván L.

AU - Fuentes, Juan A.

AU - Guzmán-Durán, Ana María

AU - Alvarez-Lobos, Manuel

AU - Paredes-Sabja, Daniel

PY - 2015/12/1

Y1 - 2015/12/1

N2 - One of the main clinical challenges of Clostridium difficile infections (CDI) is the high rate of relapse episodes. The main determinants involved in relapse of CDI include the presence of antibiotic-resistant C. difficile spores in the colonic environment and a permanent state of dysbiosis of the microbiota caused by antibiotic therapy. A possible scenario is that phenotypes related to the persistence of C. difficile spores might contribute to relapsing infections. In this study, 8 C. difficile isolates recovered from 4 cases with relapsing infection, and 9 isolates recovered from single infection cases were analyzed for PCR ribotyping and the presence of tcdA, tcdB and cdtAB genes. Factors associated to spore persistence, sporulation, spore adherence and biofilm formation and sporulation during biofilm formation were characterized. We also evaluated motility and cytotoxicity. However, we observed no significant difference in the analyzed phenotypes among the different clinical outcomes, most likely due to the high variability observed among strains within clinical backgrounds in each phenotype and the small sample size. It is noteworthy that C. difficile spores adhered to similar extents to undifferentiated and differentiated Caco-2 cells. By contrast, spores of all clinical isolates tested had increased germination efficiency in presence of taurocholate, while decreased sporulation rate during biofilm development in the presence of glucose. In conclusion, these results show that, at least in this cohort of patients, the described phenotypes are not detrimental in the clinical outcome of the disease.

AB - One of the main clinical challenges of Clostridium difficile infections (CDI) is the high rate of relapse episodes. The main determinants involved in relapse of CDI include the presence of antibiotic-resistant C. difficile spores in the colonic environment and a permanent state of dysbiosis of the microbiota caused by antibiotic therapy. A possible scenario is that phenotypes related to the persistence of C. difficile spores might contribute to relapsing infections. In this study, 8 C. difficile isolates recovered from 4 cases with relapsing infection, and 9 isolates recovered from single infection cases were analyzed for PCR ribotyping and the presence of tcdA, tcdB and cdtAB genes. Factors associated to spore persistence, sporulation, spore adherence and biofilm formation and sporulation during biofilm formation were characterized. We also evaluated motility and cytotoxicity. However, we observed no significant difference in the analyzed phenotypes among the different clinical outcomes, most likely due to the high variability observed among strains within clinical backgrounds in each phenotype and the small sample size. It is noteworthy that C. difficile spores adhered to similar extents to undifferentiated and differentiated Caco-2 cells. By contrast, spores of all clinical isolates tested had increased germination efficiency in presence of taurocholate, while decreased sporulation rate during biofilm development in the presence of glucose. In conclusion, these results show that, at least in this cohort of patients, the described phenotypes are not detrimental in the clinical outcome of the disease.

KW - Biofilm

KW - Clostridium difficile

KW - Recurrent CDI

KW - Relapse

KW - Relapsing CDI

KW - Spore adherence

KW - Spore germination

KW - Sporulation

UR - http://www.scopus.com/inward/record.url?scp=84944250383&partnerID=8YFLogxK

U2 - 10.1016/j.anaerobe.2015.09.005

DO - 10.1016/j.anaerobe.2015.09.005

M3 - Article

C2 - 26403333

AN - SCOPUS:84944250383

VL - 36

SP - 30

EP - 38

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -

Plaza-Garrido Á, Miranda-Cárdenas C, Castro-Córdova P, Olguín-Araneda V, Cofré-Araneda G, Hernández-Rocha C y otros. Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes. Anaerobe. 2015 dic 1;36:30-38. https://doi.org/10.1016/j.anaerobe.2015.09.005