Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Resultado de la investigación: Contribución a los tipos de informe/libroCapítulo

Resumen

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Idioma originalInglés
Título de la publicación alojadaPlant Cell Expansion
Subtítulo de la publicación alojadaMethods and Protocols
EditorialSpringer New York LLC
Páginas41-47
Número de páginas7
ISBN (versión digital)9781493919024
ISBN (versión impresa)9781493919017
DOI
EstadoPublicada - 18 nov 2014

Áreas temáticas de ASJC Scopus

  • Agricultura y biología (todo)
  • Medicina (todo)

Huella Profundice en los temas de investigación de 'Optimized method for growing in vitro Arabidopsis thaliana pollen tubes'. En conjunto forman una huella única.

  • Citar esto

    Borassi, C., Di Giorgio, J. P., Scarpin, M. R., Muschietti, J., & Estevez, J. M. (2014). Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. En Plant Cell Expansion: Methods and Protocols (pp. 41-47). Springer New York LLC. https://doi.org/10.1007/978-1-4939-1902-4_3