Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Resultado de la investigación: Chapter

Resumen

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Idioma originalEnglish
Título de la publicación alojadaPlant Cell Expansion
Subtítulo de la publicación alojadaMethods and Protocols
EditorialSpringer New York LLC
Páginas41-47
Número de páginas7
ISBN (versión digital)9781493919024
ISBN (versión impresa)9781493919017
DOI
EstadoPublished - 18 nov 2014

Huella dactilar

Pollen Tube
pollen tubes
Arabidopsis
Arabidopsis thaliana
Growth
methodology
Ovule
Cell Polarity
Insertional Mutagenesis
ovules
Fertilization
Spermatozoa
In Vitro Techniques
reactive oxygen species
Reactive Oxygen Species
spermatozoa
Ions
ions
Calcium
Phenotype

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Citar esto

Borassi, C., Di Giorgio, J. P., Scarpin, M. R., Muschietti, J., & Estevez, J. M. (2014). Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. En Plant Cell Expansion: Methods and Protocols (pp. 41-47). Springer New York LLC. https://doi.org/10.1007/978-1-4939-1902-4_3
Borassi, Cecilia ; Di Giorgio, Juliana Pérez ; Scarpin, María R. ; Muschietti, Jorge ; Estevez, José M. / Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. Plant Cell Expansion: Methods and Protocols. Springer New York LLC, 2014. pp. 41-47
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Borassi, C, Di Giorgio, JP, Scarpin, MR, Muschietti, J & Estevez, JM 2014, Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. En Plant Cell Expansion: Methods and Protocols. Springer New York LLC, pp. 41-47. https://doi.org/10.1007/978-1-4939-1902-4_3

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. / Borassi, Cecilia; Di Giorgio, Juliana Pérez; Scarpin, María R.; Muschietti, Jorge; Estevez, José M.

Plant Cell Expansion: Methods and Protocols. Springer New York LLC, 2014. p. 41-47.

Resultado de la investigación: Chapter

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AU - Muschietti, Jorge

AU - Estevez, José M.

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Borassi C, Di Giorgio JP, Scarpin MR, Muschietti J, Estevez JM. Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. En Plant Cell Expansion: Methods and Protocols. Springer New York LLC. 2014. p. 41-47 https://doi.org/10.1007/978-1-4939-1902-4_3