Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi; Juliana Pérez Di Giorgio; María R. Scarpin; Jorge Muschietti; José M. Estevez

doi:10.1007/978-1-4939-1902-4_3

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Facultad de Ciencias de la Vida

Producción científica: Contribución a los tipos de informe/libro › Capítulo › revisión exhaustiva

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Resumen

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Idioma original	Inglés
Título de la publicación alojada	Plant Cell Expansion
Subtítulo de la publicación alojada	Methods and Protocols
Editorial	Springer New York LLC
Páginas	41-47
Número de páginas	7
ISBN (versión digital)	9781493919024
ISBN (versión impresa)	9781493919017
DOI	https://doi.org/10.1007/978-1-4939-1902-4_3
Estado	Publicada - 18 nov. 2014

Áreas temáticas de ASJC Scopus

Ciencias Agrícolas y Biológicas General
Medicina General

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abstract = "Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.",

keywords = "Arabidopsis pollen, Cell expansion, Pollen tube growth",

author = "Cecilia Borassi and {Di Giorgio}, {Juliana P{\'e}rez} and Scarpin, {Mar{\'i}a R.} and Jorge Muschietti and Estevez, {Jos{\'e} M.}",

year = "2014",

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AU - Borassi, Cecilia

AU - Di Giorgio, Juliana Pérez

AU - Scarpin, María R.

AU - Muschietti, Jorge

AU - Estevez, José M.

PY - 2014/11/18

Y1 - 2014/11/18

N2 - Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

AB - Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

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KW - Cell expansion

KW - Pollen tube growth

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Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

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