Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Idioma originalInglés
Páginas (desde-hasta)41-47
Número de páginas7
PublicaciónMethods in Molecular Biology
Volumen1242
DOI
EstadoPublicada - 1 ene 2015

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Genética

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