Opposite fate of endocytosed CCR7 and its ligands: Recycling versus degradation

Carolina Otero, Marcus Groettrup, Daniel F. Legler

Resultado de la investigación: Article

79 Citas (Scopus)

Resumen

The chemokine receptor CCR7 and its ligands CCL19 and CCL21 play a crucial role for the homing of lymphocytes and dendritic cells to secondary lymphoid tissues. Nevertheless, how CCR7 senses the gradient of chemokines and how migration is terminated are poorly understood. In this study, we demonstrate that CCR7(-GFP) is endocytosed into early endosomes containing transferrin receptor upon CCL19 binding, but less upon CCL21 triggering. Internalization of CCR7 was independent of lipid rafts but relied on dynamin and Eps15 and was inhibited by hypertonic sucrose, suggesting clathrin-dependent endocytosis. After chemokine removal, internalized CCR7 recycled back to the plasma membrane and was able to mediate migration again. In contrast, internalized CCL19 was sorted to lysosomes for degradation, showing opposite fate for endocytosed CCR7 and its ligand.

Idioma originalEnglish
Páginas (desde-hasta)2314-2323
Número de páginas10
PublicaciónJournal of Immunology
Volumen177
N.º4
EstadoPublished - 15 ago 2006

Huella dactilar

Recycling
Endocytosis
Ligands
Chemokines
Dynamins
Clathrin
Transferrin Receptors
Chemokine Receptors
Endosomes
Lymphoid Tissue
Lysosomes
Dendritic Cells
Sucrose
Cell Membrane
Lymphocytes
Lipids

ASJC Scopus subject areas

  • Immunology

Citar esto

Otero, Carolina ; Groettrup, Marcus ; Legler, Daniel F. / Opposite fate of endocytosed CCR7 and its ligands : Recycling versus degradation. En: Journal of Immunology. 2006 ; Vol. 177, N.º 4. pp. 2314-2323.
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Otero, C, Groettrup, M & Legler, DF 2006, 'Opposite fate of endocytosed CCR7 and its ligands: Recycling versus degradation', Journal of Immunology, vol. 177, n.º 4, pp. 2314-2323.

Opposite fate of endocytosed CCR7 and its ligands : Recycling versus degradation. / Otero, Carolina; Groettrup, Marcus; Legler, Daniel F.

En: Journal of Immunology, Vol. 177, N.º 4, 15.08.2006, p. 2314-2323.

Resultado de la investigación: Article

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AB - The chemokine receptor CCR7 and its ligands CCL19 and CCL21 play a crucial role for the homing of lymphocytes and dendritic cells to secondary lymphoid tissues. Nevertheless, how CCR7 senses the gradient of chemokines and how migration is terminated are poorly understood. In this study, we demonstrate that CCR7(-GFP) is endocytosed into early endosomes containing transferrin receptor upon CCL19 binding, but less upon CCL21 triggering. Internalization of CCR7 was independent of lipid rafts but relied on dynamin and Eps15 and was inhibited by hypertonic sucrose, suggesting clathrin-dependent endocytosis. After chemokine removal, internalized CCR7 recycled back to the plasma membrane and was able to mediate migration again. In contrast, internalized CCL19 was sorted to lysosomes for degradation, showing opposite fate for endocytosed CCR7 and its ligand.

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