Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex

Paulo C. Covarrubias, Ana Moya-Beltrán, Joaquin Atavales, Francisco Moya-Flores, Pablo S. Tapia, Lillian G. Acuña, Silvia Spinelli, Raquel Quatrini

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

General knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30%). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservation of the virus across strains of the A. caldus species. Such conservation extends from the modules architecture to the gene level, suggesting that organization and composition of these viruses are preserved for functional reasons. Accordingly, the AcaML1 proviruses were demonstrated to excise from their host genomes under DNA-damaging conditions triggering the SOS-response and to produce DNA-containing VLPs. Despite this fact, under the conditions evaluated (acidic) the VLPs obtained from A. caldus ATCC 51756 could not produce productive infections of a candidate sensitive strain (#6) nor trigger it lysis.

Idioma originalEnglish
Páginas (desde-hasta)628-637
Número de páginas10
PublicaciónResearch in Microbiology
Volumen169
N.º10
DOI
EstadoPublished - 1 dic 2018

Huella dactilar

Acidithiobacillus
Proviruses
Viruses
DNA
Genes
Organizations
Genome
Infection

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Covarrubias, P. C., Moya-Beltrán, A., Atavales, J., Moya-Flores, F., Tapia, P. S., Acuña, L. G., ... Quatrini, R. (2018). Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex. Research in Microbiology, 169(10), 628-637. https://doi.org/10.1016/j.resmic.2018.07.005
Covarrubias, Paulo C. ; Moya-Beltrán, Ana ; Atavales, Joaquin ; Moya-Flores, Francisco ; Tapia, Pablo S. ; Acuña, Lillian G. ; Spinelli, Silvia ; Quatrini, Raquel. / Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex. En: Research in Microbiology. 2018 ; Vol. 169, N.º 10. pp. 628-637.
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title = "Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex",
abstract = "General knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30{\%}). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservation of the virus across strains of the A. caldus species. Such conservation extends from the modules architecture to the gene level, suggesting that organization and composition of these viruses are preserved for functional reasons. Accordingly, the AcaML1 proviruses were demonstrated to excise from their host genomes under DNA-damaging conditions triggering the SOS-response and to produce DNA-containing VLPs. Despite this fact, under the conditions evaluated (acidic) the VLPs obtained from A. caldus ATCC 51756 could not produce productive infections of a candidate sensitive strain (#6) nor trigger it lysis.",
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Covarrubias, PC, Moya-Beltrán, A, Atavales, J, Moya-Flores, F, Tapia, PS, Acuña, LG, Spinelli, S & Quatrini, R 2018, 'Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex', Research in Microbiology, vol. 169, n.º 10, pp. 628-637. https://doi.org/10.1016/j.resmic.2018.07.005

Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex. / Covarrubias, Paulo C.; Moya-Beltrán, Ana; Atavales, Joaquin; Moya-Flores, Francisco; Tapia, Pablo S.; Acuña, Lillian G.; Spinelli, Silvia; Quatrini, Raquel.

En: Research in Microbiology, Vol. 169, N.º 10, 01.12.2018, p. 628-637.

Resultado de la investigación: Article

TY - JOUR

T1 - Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex

AU - Covarrubias, Paulo C.

AU - Moya-Beltrán, Ana

AU - Atavales, Joaquin

AU - Moya-Flores, Francisco

AU - Tapia, Pablo S.

AU - Acuña, Lillian G.

AU - Spinelli, Silvia

AU - Quatrini, Raquel

PY - 2018/12/1

Y1 - 2018/12/1

N2 - General knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30%). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservation of the virus across strains of the A. caldus species. Such conservation extends from the modules architecture to the gene level, suggesting that organization and composition of these viruses are preserved for functional reasons. Accordingly, the AcaML1 proviruses were demonstrated to excise from their host genomes under DNA-damaging conditions triggering the SOS-response and to produce DNA-containing VLPs. Despite this fact, under the conditions evaluated (acidic) the VLPs obtained from A. caldus ATCC 51756 could not produce productive infections of a candidate sensitive strain (#6) nor trigger it lysis.

AB - General knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30%). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservation of the virus across strains of the A. caldus species. Such conservation extends from the modules architecture to the gene level, suggesting that organization and composition of these viruses are preserved for functional reasons. Accordingly, the AcaML1 proviruses were demonstrated to excise from their host genomes under DNA-damaging conditions triggering the SOS-response and to produce DNA-containing VLPs. Despite this fact, under the conditions evaluated (acidic) the VLPs obtained from A. caldus ATCC 51756 could not produce productive infections of a candidate sensitive strain (#6) nor trigger it lysis.

KW - A. caldus

KW - Acidithiobacilli

KW - Bacteriophage

KW - PFGE

KW - T. tepidarius

KW - VLPs

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