O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez, Martiniano M. Ricardi, Javier Gloazzo Dorosz, Paula V. Fernandez, Alejandro D. Nadra, Laercio Pol-Fachin, Jack Egelund, Sascha Gille, Jesper Harholt, Marina Ciancia, Hugo Verli, Markus Pauly, Antony Bacic, Carl Erik Olsen, Peter Ulvskov, Bent Larsen Petersen, Chris Somerville, Norberto D. Iusem, Jose M. Estevez

Resultado de la investigación: Contribución a una revistaArtículo

157 Citas (Scopus)

Resumen

Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

Idioma originalInglés
Páginas (desde-hasta)1401-1403
Número de páginas3
PublicaciónScience
Volumen332
N.º6036
DOI
EstadoPublicada - 17 jun 2011
Publicado de forma externa

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    Velasquez, S. M., Ricardi, M. M., Dorosz, J. G., Fernandez, P. V., Nadra, A. D., Pol-Fachin, L., Egelund, J., Gille, S., Harholt, J., Ciancia, M., Verli, H., Pauly, M., Bacic, A., Olsen, C. E., Ulvskov, P., Petersen, B. L., Somerville, C., Iusem, N. D., & Estevez, J. M. (2011). O-glycosylated cell wall proteins are essential in root hair growth. Science, 332(6036), 1401-1403. https://doi.org/10.1126/science.1206657