O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez, Martiniano M. Ricardi, Javier Gloazzo Dorosz, Paula V. Fernandez, Alejandro D. Nadra, Laercio Pol-Fachin, Jack Egelund, Sascha Gille, Jesper Harholt, Marina Ciancia, Hugo Verli, Markus Pauly, Antony Bacic, Carl Erik Olsen, Peter Ulvskov, Bent Larsen Petersen, Chris Somerville, Norberto D. Iusem, Jose M. Estevez

Resultado de la investigación: Article

149 Citas (Scopus)

Resumen

Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

Idioma originalEnglish
Páginas (desde-hasta)1401-1403
Número de páginas3
PublicaciónScience
Volumen332
N.º6036
DOI
EstadoPublished - 17 jun 2011
Publicado de forma externa

Huella dactilar

Cell Wall
Prolyl Hydroxylases
Hydroxyproline
Glycosylation
Glycoproteins
Growth
Proteins
Hydroxylation
Post Translational Protein Processing
Arabidopsis
Proline
Polysaccharides
Food
arabinogalactan proteins
arabinosyltransferase

ASJC Scopus subject areas

  • General

Citar esto

Velasquez, S. M., Ricardi, M. M., Dorosz, J. G., Fernandez, P. V., Nadra, A. D., Pol-Fachin, L., ... Estevez, J. M. (2011). O-glycosylated cell wall proteins are essential in root hair growth. Science, 332(6036), 1401-1403. https://doi.org/10.1126/science.1206657
Velasquez, Silvia M. ; Ricardi, Martiniano M. ; Dorosz, Javier Gloazzo ; Fernandez, Paula V. ; Nadra, Alejandro D. ; Pol-Fachin, Laercio ; Egelund, Jack ; Gille, Sascha ; Harholt, Jesper ; Ciancia, Marina ; Verli, Hugo ; Pauly, Markus ; Bacic, Antony ; Olsen, Carl Erik ; Ulvskov, Peter ; Petersen, Bent Larsen ; Somerville, Chris ; Iusem, Norberto D. ; Estevez, Jose M. / O-glycosylated cell wall proteins are essential in root hair growth. En: Science. 2011 ; Vol. 332, N.º 6036. pp. 1401-1403.
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title = "O-glycosylated cell wall proteins are essential in root hair growth",
abstract = "Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.",
author = "Velasquez, {Silvia M.} and Ricardi, {Martiniano M.} and Dorosz, {Javier Gloazzo} and Fernandez, {Paula V.} and Nadra, {Alejandro D.} and Laercio Pol-Fachin and Jack Egelund and Sascha Gille and Jesper Harholt and Marina Ciancia and Hugo Verli and Markus Pauly and Antony Bacic and Olsen, {Carl Erik} and Peter Ulvskov and Petersen, {Bent Larsen} and Chris Somerville and Iusem, {Norberto D.} and Estevez, {Jose M.}",
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Velasquez, SM, Ricardi, MM, Dorosz, JG, Fernandez, PV, Nadra, AD, Pol-Fachin, L, Egelund, J, Gille, S, Harholt, J, Ciancia, M, Verli, H, Pauly, M, Bacic, A, Olsen, CE, Ulvskov, P, Petersen, BL, Somerville, C, Iusem, ND & Estevez, JM 2011, 'O-glycosylated cell wall proteins are essential in root hair growth', Science, vol. 332, n.º 6036, pp. 1401-1403. https://doi.org/10.1126/science.1206657

O-glycosylated cell wall proteins are essential in root hair growth. / Velasquez, Silvia M.; Ricardi, Martiniano M.; Dorosz, Javier Gloazzo; Fernandez, Paula V.; Nadra, Alejandro D.; Pol-Fachin, Laercio; Egelund, Jack; Gille, Sascha; Harholt, Jesper; Ciancia, Marina; Verli, Hugo; Pauly, Markus; Bacic, Antony; Olsen, Carl Erik; Ulvskov, Peter; Petersen, Bent Larsen; Somerville, Chris; Iusem, Norberto D.; Estevez, Jose M.

En: Science, Vol. 332, N.º 6036, 17.06.2011, p. 1401-1403.

Resultado de la investigación: Article

TY - JOUR

T1 - O-glycosylated cell wall proteins are essential in root hair growth

AU - Velasquez, Silvia M.

AU - Ricardi, Martiniano M.

AU - Dorosz, Javier Gloazzo

AU - Fernandez, Paula V.

AU - Nadra, Alejandro D.

AU - Pol-Fachin, Laercio

AU - Egelund, Jack

AU - Gille, Sascha

AU - Harholt, Jesper

AU - Ciancia, Marina

AU - Verli, Hugo

AU - Pauly, Markus

AU - Bacic, Antony

AU - Olsen, Carl Erik

AU - Ulvskov, Peter

AU - Petersen, Bent Larsen

AU - Somerville, Chris

AU - Iusem, Norberto D.

AU - Estevez, Jose M.

PY - 2011/6/17

Y1 - 2011/6/17

N2 - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

AB - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

UR - http://www.scopus.com/inward/record.url?scp=79959356947&partnerID=8YFLogxK

U2 - 10.1126/science.1206657

DO - 10.1126/science.1206657

M3 - Article

C2 - 21680836

AN - SCOPUS:79959356947

VL - 332

SP - 1401

EP - 1403

JO - Science

JF - Science

SN - 0036-8075

IS - 6036

ER -

Velasquez SM, Ricardi MM, Dorosz JG, Fernandez PV, Nadra AD, Pol-Fachin L y otros. O-glycosylated cell wall proteins are essential in root hair growth. Science. 2011 jun 17;332(6036):1401-1403. https://doi.org/10.1126/science.1206657