Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system

Jorge Villanueva, Paulina Bull, Pablo Valenzuela, Alejandro Venegas

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Twelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.

Idioma originalEnglish
Páginas (desde-hasta)165-169
Número de páginas5
PublicaciónFEBS Letters
Volumen167
N.º1
DOI
EstadoPublished - 13 feb 1984

Huella dactilar

Transcription
Yeast
Nucleotides
Genes
Yeasts
RNA, Transfer, Asp
Clone Cells
Gene Library
DNA sequences
Genome
In Vitro Techniques

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Genetics
  • Cell Biology

Citar esto

Villanueva, Jorge ; Bull, Paulina ; Valenzuela, Pablo ; Venegas, Alejandro. / Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system. En: FEBS Letters. 1984 ; Vol. 167, N.º 1. pp. 165-169.
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Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system. / Villanueva, Jorge; Bull, Paulina; Valenzuela, Pablo; Venegas, Alejandro.

En: FEBS Letters, Vol. 167, N.º 1, 13.02.1984, p. 165-169.

Resultado de la investigación: Article

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KW - Homologous in vitro transcription

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