Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system

Jorge Villanueva, Paulina Bull, Pablo Valenzuela, Alejandro Venegas

Resultado de la investigación: Contribución a una revistaArtículo

2 Citas (Scopus)

Resumen

Twelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.

Idioma originalInglés
Páginas (desde-hasta)165-169
Número de páginas5
PublicaciónFEBS Letters
Volumen167
N.º1
DOI
EstadoPublicada - 13 feb 1984

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Biología estructural
  • Bioquímica
  • Medicina (todo)
  • Biología molecular
  • Genética
  • Biología celular

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