Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells

Eduardo Landerer, Jaime Villegas, Veronica A. Burzio, Luciana Oliveira, Claudio Villota, Constanza Lopez, Franko Restovic, Ronny Martinez, Octavio Castillo, Luis O. Burzio

Resultado de la investigación: Contribución a una revistaArtículo

46 Citas (Scopus)

Resumen

Background We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. Methods FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. Results In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. Conclusion The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.

Idioma originalInglés
Páginas (desde-hasta)297-305
Número de páginas9
PublicaciónCellular Oncology
Volumen34
N.º4
DOI
EstadoPublicada - ago 2011

Áreas temáticas de ASJC Scopus

  • Medicina molecular
  • Oncología
  • Investigación sobre el cáncer
  • Medicina (todo)

Huella Profundice en los temas de investigación de 'Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells'. En conjunto forman una huella única.

  • Citar esto

    Landerer, E., Villegas, J., Burzio, V. A., Oliveira, L., Villota, C., Lopez, C., Restovic, F., Martinez, R., Castillo, O., & Burzio, L. O. (2011). Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells. Cellular Oncology, 34(4), 297-305. https://doi.org/10.1007/s13402-011-0018-8