Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells

Eduardo Landerer, Jaime Villegas, Veronica A. Burzio, Luciana Oliveira, Claudio Villota, Constanza Lopez, Franko Restovic, Ronny Martinez, Octavio Castillo, Luis O. Burzio

Resultado de la investigación: Article

40 Citas (Scopus)

Resumen

Background We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. Methods FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. Results In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. Conclusion The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.

Idioma originalEnglish
Páginas (desde-hasta)297-305
Número de páginas9
PublicaciónCellular Oncology
Volumen34
N.º4
DOI
EstadoPublished - ago 2011

Huella dactilar

Neoplasms
In Situ Hybridization
Heterochromatin
Ribonucleases
Confocal Microscopy
Organelles
Testis
Digestion
Electron Microscopy
Mitochondria
Down-Regulation
Communication
Kidney

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Cancer Research
  • Medicine(all)

Citar esto

Landerer, Eduardo ; Villegas, Jaime ; Burzio, Veronica A. ; Oliveira, Luciana ; Villota, Claudio ; Lopez, Constanza ; Restovic, Franko ; Martinez, Ronny ; Castillo, Octavio ; Burzio, Luis O. / Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells. En: Cellular Oncology. 2011 ; Vol. 34, N.º 4. pp. 297-305.
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title = "Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells",
abstract = "Background We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. Methods FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. Results In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. Conclusion The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.",
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author = "Eduardo Landerer and Jaime Villegas and Burzio, {Veronica A.} and Luciana Oliveira and Claudio Villota and Constanza Lopez and Franko Restovic and Ronny Martinez and Octavio Castillo and Burzio, {Luis O.}",
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Landerer, E, Villegas, J, Burzio, VA, Oliveira, L, Villota, C, Lopez, C, Restovic, F, Martinez, R, Castillo, O & Burzio, LO 2011, 'Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells', Cellular Oncology, vol. 34, n.º 4, pp. 297-305. https://doi.org/10.1007/s13402-011-0018-8

Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells. / Landerer, Eduardo; Villegas, Jaime; Burzio, Veronica A.; Oliveira, Luciana; Villota, Claudio; Lopez, Constanza; Restovic, Franko; Martinez, Ronny; Castillo, Octavio; Burzio, Luis O.

En: Cellular Oncology, Vol. 34, N.º 4, 08.2011, p. 297-305.

Resultado de la investigación: Article

TY - JOUR

T1 - Nuclear localization of the mitochondrial ncRNAs in normal and cancer cells

AU - Landerer, Eduardo

AU - Villegas, Jaime

AU - Burzio, Veronica A.

AU - Oliveira, Luciana

AU - Villota, Claudio

AU - Lopez, Constanza

AU - Restovic, Franko

AU - Martinez, Ronny

AU - Castillo, Octavio

AU - Burzio, Luis O.

PY - 2011/8

Y1 - 2011/8

N2 - Background We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. Methods FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. Results In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. Conclusion The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.

AB - Background We have previously shown a differential expression of a family of mitochondrial ncRNAs in normal and cancer cells. Normal proliferating cells and cancer cells express the sense mitochondrial ncRNA (SncmtRNA). In addition, while normal proliferating cells express two antisense mitochondrial ncRNAs (ASncmtRNAs-1 and -2), these transcripts seem to be universally down-regulated in cancer cells. In situ hybridization (ISH) of some normal and cancer tissues reveals nuclear localization of these transcripts suggesting that they are exported from mitochondria. Methods FISH and confocal microscopy, in situ digestion with RNase previous to ISH and electron microscopy ISH was employed to confirm the extra-mitochondrial localization of the SncmtRNA and the ASncmtRNAs in normal proliferating and cancer cells of human and mouse. Results In normal human kidney and mouse testis the SncmtRNA and the ASncmtRNAs were found outside the organelle and especially localized in the nucleus associated to heterochromatin. In cancer cells, only the SncmtRNA was expressed and was found associated to heterochromatin and nucleoli. Conclusion The ubiquitous localization of these mitochondrial transcripts in the nucleus suggests that they are new players in the mitochondrial-nuclear communication pathway or retrograde signaling. Down regulation of the ASncmtRNAs seems to be an important step on neoplastic transformation and cancer progression.

KW - Cancer

KW - Mitochondria

KW - NcRNAs

KW - Nuclear localization

KW - Retrograde signaling

UR - http://www.scopus.com/inward/record.url?scp=84862277713&partnerID=8YFLogxK

U2 - 10.1007/s13402-011-0018-8

DO - 10.1007/s13402-011-0018-8

M3 - Article

VL - 34

SP - 297

EP - 305

JO - Cellular oncology (Dordrecht)

JF - Cellular oncology (Dordrecht)

SN - 2211-3428

IS - 4

ER -