NPR1-independent activation of immediate early salicylic acid-responsive genes in Arabidopsis

Carolina Uquillas, Ingrid Letelier, Francisca Blanco, Xavier Jordana, Loreto Holuigue

Resultado de la investigación: Article

76 Citas (Scopus)

Resumen

Salicylic acid (SA) is a key signal for the activation of defense genes in response to stress. The activation of late defense genes by SA, such as PR-1, involves the participation of the NPR1 protein. This protein acts as coactivator of the TGA factors that recognize as-1-like elements in the PR-1 promoter. Considering that functional as-1-like elements are also found in the promoter of SA- and auxin-responsive immediate early genes, we tested the hypothesis that NPR1 is also required for activation of these genes. The expression of the immediate early genes glutathione S-transferase (GST6) and glucosyltransferase (EIGT) was studied in npr1 mutant and wild-type Arabidopsis plants. In the npr1 mutant background, SA and 2,4-dichlorophenoxyacetic acid were unable to promote transcription of PR-1 but effectively stimulated the expression of GST6 and EIGT. Furthermore, increased binding of proteins to the GST6 as-1-like promoter element was detected in nuclear extracts from npr1 and wild-type plants after treatment with SA. In summary, these results indicate that activation of immediate early genes by SA proceeds through an NPR1-independent pathway. Therefore, we propose that activation by SA of immediate early and late genes occur by different mechanisms.

Idioma originalEnglish
Páginas (desde-hasta)34-42
Número de páginas9
PublicaciónMolecular Plant-Microbe Interactions
Volumen17
N.º1
EstadoPublished - ene 2004

Huella dactilar

Salicylic Acid
Arabidopsis
salicylic acid
Genes
Chemical activation
Immediate-Early Genes
genes
promoter regions
Transcriptional Activation
Glucosyltransferases
mutants
glucosyltransferases
2,4-Dichlorophenoxyacetic Acid
Indoleacetic Acids
gene activation
Transcription
Glutathione Transferase
glutathione transferase
2,4-D
binding proteins

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Agronomy and Crop Science
  • Physiology
  • Medicine(all)

Citar esto

Uquillas, Carolina ; Letelier, Ingrid ; Blanco, Francisca ; Jordana, Xavier ; Holuigue, Loreto. / NPR1-independent activation of immediate early salicylic acid-responsive genes in Arabidopsis. En: Molecular Plant-Microbe Interactions. 2004 ; Vol. 17, N.º 1. pp. 34-42.
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abstract = "Salicylic acid (SA) is a key signal for the activation of defense genes in response to stress. The activation of late defense genes by SA, such as PR-1, involves the participation of the NPR1 protein. This protein acts as coactivator of the TGA factors that recognize as-1-like elements in the PR-1 promoter. Considering that functional as-1-like elements are also found in the promoter of SA- and auxin-responsive immediate early genes, we tested the hypothesis that NPR1 is also required for activation of these genes. The expression of the immediate early genes glutathione S-transferase (GST6) and glucosyltransferase (EIGT) was studied in npr1 mutant and wild-type Arabidopsis plants. In the npr1 mutant background, SA and 2,4-dichlorophenoxyacetic acid were unable to promote transcription of PR-1 but effectively stimulated the expression of GST6 and EIGT. Furthermore, increased binding of proteins to the GST6 as-1-like promoter element was detected in nuclear extracts from npr1 and wild-type plants after treatment with SA. In summary, these results indicate that activation of immediate early genes by SA proceeds through an NPR1-independent pathway. Therefore, we propose that activation by SA of immediate early and late genes occur by different mechanisms.",
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NPR1-independent activation of immediate early salicylic acid-responsive genes in Arabidopsis. / Uquillas, Carolina; Letelier, Ingrid; Blanco, Francisca; Jordana, Xavier; Holuigue, Loreto.

En: Molecular Plant-Microbe Interactions, Vol. 17, N.º 1, 01.2004, p. 34-42.

Resultado de la investigación: Article

TY - JOUR

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AU - Uquillas, Carolina

AU - Letelier, Ingrid

AU - Blanco, Francisca

AU - Jordana, Xavier

AU - Holuigue, Loreto

PY - 2004/1

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N2 - Salicylic acid (SA) is a key signal for the activation of defense genes in response to stress. The activation of late defense genes by SA, such as PR-1, involves the participation of the NPR1 protein. This protein acts as coactivator of the TGA factors that recognize as-1-like elements in the PR-1 promoter. Considering that functional as-1-like elements are also found in the promoter of SA- and auxin-responsive immediate early genes, we tested the hypothesis that NPR1 is also required for activation of these genes. The expression of the immediate early genes glutathione S-transferase (GST6) and glucosyltransferase (EIGT) was studied in npr1 mutant and wild-type Arabidopsis plants. In the npr1 mutant background, SA and 2,4-dichlorophenoxyacetic acid were unable to promote transcription of PR-1 but effectively stimulated the expression of GST6 and EIGT. Furthermore, increased binding of proteins to the GST6 as-1-like promoter element was detected in nuclear extracts from npr1 and wild-type plants after treatment with SA. In summary, these results indicate that activation of immediate early genes by SA proceeds through an NPR1-independent pathway. Therefore, we propose that activation by SA of immediate early and late genes occur by different mechanisms.

AB - Salicylic acid (SA) is a key signal for the activation of defense genes in response to stress. The activation of late defense genes by SA, such as PR-1, involves the participation of the NPR1 protein. This protein acts as coactivator of the TGA factors that recognize as-1-like elements in the PR-1 promoter. Considering that functional as-1-like elements are also found in the promoter of SA- and auxin-responsive immediate early genes, we tested the hypothesis that NPR1 is also required for activation of these genes. The expression of the immediate early genes glutathione S-transferase (GST6) and glucosyltransferase (EIGT) was studied in npr1 mutant and wild-type Arabidopsis plants. In the npr1 mutant background, SA and 2,4-dichlorophenoxyacetic acid were unable to promote transcription of PR-1 but effectively stimulated the expression of GST6 and EIGT. Furthermore, increased binding of proteins to the GST6 as-1-like promoter element was detected in nuclear extracts from npr1 and wild-type plants after treatment with SA. In summary, these results indicate that activation of immediate early genes by SA proceeds through an NPR1-independent pathway. Therefore, we propose that activation by SA of immediate early and late genes occur by different mechanisms.

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