Non-cytotoxic copper overload boosts mitochondrial energy metabolism to modulate cell proliferation and differentiation in the human erythroleukemic cell line K562

Lina M. Ruiz, Erik L. Jensen, Yancing Rossel, German I. Puas, Alvaro M. Gonzalez-Ibanez, Rodrigo I. Bustos, David A. Ferrick, Alvaro A. Elorza

Resultado de la investigación: Article

10 Citas (Scopus)

Resumen

Copper is integral to the mitochondrial respiratory complex IV and contributes to proliferation and differentiation, metabolic reprogramming and mitochondrial function. The K562 cell line was exposed to a non-cytotoxic copper overload to evaluate mitochondrial dynamics, function and cell fate. This induced higher rates of mitochondrial turnover given by an increase in mitochondrial fusion and fission events and in the autophagic flux. The appearance of smaller and condensed mitochondria was also observed. Bioenergetics activity included more respiratory complexes, higher oxygen consumption rate, superoxide production and ATP synthesis, with no decrease in membrane potential. Increased cell proliferation and inhibited differentiation also occurred. Non-cytotoxic copper levels can modify mitochondrial metabolism and cell fate, which could be used in cancer biology and regenerative medicine.

Idioma originalEnglish
Páginas (desde-hasta)18-30
Número de páginas13
PublicaciónMitochondrion
Volumen29
DOI
EstadoPublished - 1 jul 2016

Huella dactilar

Mitochondrial Dynamics
Energy Metabolism
Copper
Cell Differentiation
Cell Proliferation
Cell Line
Mitochondrial Turnover
Regenerative Medicine
K562 Cells
Oxygen Consumption
Superoxides
Membrane Potentials
Mitochondria
Adenosine Triphosphate
Neoplasms

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Citar esto

Ruiz, Lina M. ; Jensen, Erik L. ; Rossel, Yancing ; Puas, German I. ; Gonzalez-Ibanez, Alvaro M. ; Bustos, Rodrigo I. ; Ferrick, David A. ; Elorza, Alvaro A. / Non-cytotoxic copper overload boosts mitochondrial energy metabolism to modulate cell proliferation and differentiation in the human erythroleukemic cell line K562. En: Mitochondrion. 2016 ; Vol. 29. pp. 18-30.
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abstract = "Copper is integral to the mitochondrial respiratory complex IV and contributes to proliferation and differentiation, metabolic reprogramming and mitochondrial function. The K562 cell line was exposed to a non-cytotoxic copper overload to evaluate mitochondrial dynamics, function and cell fate. This induced higher rates of mitochondrial turnover given by an increase in mitochondrial fusion and fission events and in the autophagic flux. The appearance of smaller and condensed mitochondria was also observed. Bioenergetics activity included more respiratory complexes, higher oxygen consumption rate, superoxide production and ATP synthesis, with no decrease in membrane potential. Increased cell proliferation and inhibited differentiation also occurred. Non-cytotoxic copper levels can modify mitochondrial metabolism and cell fate, which could be used in cancer biology and regenerative medicine.",
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Non-cytotoxic copper overload boosts mitochondrial energy metabolism to modulate cell proliferation and differentiation in the human erythroleukemic cell line K562. / Ruiz, Lina M.; Jensen, Erik L.; Rossel, Yancing; Puas, German I.; Gonzalez-Ibanez, Alvaro M.; Bustos, Rodrigo I.; Ferrick, David A.; Elorza, Alvaro A.

En: Mitochondrion, Vol. 29, 01.07.2016, p. 18-30.

Resultado de la investigación: Article

TY - JOUR

T1 - Non-cytotoxic copper overload boosts mitochondrial energy metabolism to modulate cell proliferation and differentiation in the human erythroleukemic cell line K562

AU - Ruiz, Lina M.

AU - Jensen, Erik L.

AU - Rossel, Yancing

AU - Puas, German I.

AU - Gonzalez-Ibanez, Alvaro M.

AU - Bustos, Rodrigo I.

AU - Ferrick, David A.

AU - Elorza, Alvaro A.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Copper is integral to the mitochondrial respiratory complex IV and contributes to proliferation and differentiation, metabolic reprogramming and mitochondrial function. The K562 cell line was exposed to a non-cytotoxic copper overload to evaluate mitochondrial dynamics, function and cell fate. This induced higher rates of mitochondrial turnover given by an increase in mitochondrial fusion and fission events and in the autophagic flux. The appearance of smaller and condensed mitochondria was also observed. Bioenergetics activity included more respiratory complexes, higher oxygen consumption rate, superoxide production and ATP synthesis, with no decrease in membrane potential. Increased cell proliferation and inhibited differentiation also occurred. Non-cytotoxic copper levels can modify mitochondrial metabolism and cell fate, which could be used in cancer biology and regenerative medicine.

AB - Copper is integral to the mitochondrial respiratory complex IV and contributes to proliferation and differentiation, metabolic reprogramming and mitochondrial function. The K562 cell line was exposed to a non-cytotoxic copper overload to evaluate mitochondrial dynamics, function and cell fate. This induced higher rates of mitochondrial turnover given by an increase in mitochondrial fusion and fission events and in the autophagic flux. The appearance of smaller and condensed mitochondria was also observed. Bioenergetics activity included more respiratory complexes, higher oxygen consumption rate, superoxide production and ATP synthesis, with no decrease in membrane potential. Increased cell proliferation and inhibited differentiation also occurred. Non-cytotoxic copper levels can modify mitochondrial metabolism and cell fate, which could be used in cancer biology and regenerative medicine.

KW - Autophagy

KW - Bioenergetics

KW - Copper

KW - Erythropoiesis

KW - Mitochondria

KW - Mitochondrial dynamics

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