New insights into the heterogeneous ripening in Hass avocado via LC–MS/MS proteomics

Claudia Fuentealba, Ignacia Hernández, J. A. Olaeta, Bruno Defilippi, Claudio Meneses, Reinaldo Campos, Susan Lurie, Sebastien Carpentier, Romina Pedreschi

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Hass avocado postharvest ripening heterogeneity creates several logistics problems at destination markets. Although several approaches mainly based on targeted analysis have been undertaken to broaden our understanding of the potential causes of this postharvest ripening heterogeneity, still the secret is not yet unveiled. In this study biopsies from individual fruit displaying contrasting ripening behavior (slow vs fast) and subjected to different storage conditions (immediately after harvest, 30 d regular air at 5 °C and 30 d at 5 °C and 4 kPa O2 and 6 kPa CO2) were analyzed for targeted fatty acid analysis and cell wall enzyme activity combined with a more integrative approach based on gel free proteomics LC–MS/MS. The high throughput proteomics was capable of discriminating between slow and fast ripening avocado fruit. Proteins that were mainly less abundant in the slow ripening phenotype were annotated to correct protein folding, translation and de novo synthesis and higher abundant proteins were annotated to amino sugar and nucleotide metabolism, detoxification and stress response and proteolysis. Our study opens new insights into the causes of heterogeneous ripening and may provide new markers for avocado ripening at harvest and after prolonged storage.

Idioma originalEnglish
Páginas (desde-hasta)51-61
Número de páginas11
PublicaciónPostharvest Biology and Technology
Volumen132
DOI
EstadoPublished - 1 oct 2017

Huella dactilar

Persea
avocados
Proteomics
proteomics
ripening
Fruit
Amino Sugars
Protein Folding
Protein Biosynthesis
Cell Wall
Proteolysis
Proteins
Fatty Acids
Nucleotides
Gels
Air
Phenotype
Biopsy
amino sugars
Enzymes

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Horticulture

Citar esto

Fuentealba, Claudia ; Hernández, Ignacia ; Olaeta, J. A. ; Defilippi, Bruno ; Meneses, Claudio ; Campos, Reinaldo ; Lurie, Susan ; Carpentier, Sebastien ; Pedreschi, Romina. / New insights into the heterogeneous ripening in Hass avocado via LC–MS/MS proteomics. En: Postharvest Biology and Technology. 2017 ; Vol. 132. pp. 51-61.
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abstract = "Hass avocado postharvest ripening heterogeneity creates several logistics problems at destination markets. Although several approaches mainly based on targeted analysis have been undertaken to broaden our understanding of the potential causes of this postharvest ripening heterogeneity, still the secret is not yet unveiled. In this study biopsies from individual fruit displaying contrasting ripening behavior (slow vs fast) and subjected to different storage conditions (immediately after harvest, 30 d regular air at 5 °C and 30 d at 5 °C and 4 kPa O2 and 6 kPa CO2) were analyzed for targeted fatty acid analysis and cell wall enzyme activity combined with a more integrative approach based on gel free proteomics LC–MS/MS. The high throughput proteomics was capable of discriminating between slow and fast ripening avocado fruit. Proteins that were mainly less abundant in the slow ripening phenotype were annotated to correct protein folding, translation and de novo synthesis and higher abundant proteins were annotated to amino sugar and nucleotide metabolism, detoxification and stress response and proteolysis. Our study opens new insights into the causes of heterogeneous ripening and may provide new markers for avocado ripening at harvest and after prolonged storage.",
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Fuentealba, C, Hernández, I, Olaeta, JA, Defilippi, B, Meneses, C, Campos, R, Lurie, S, Carpentier, S & Pedreschi, R 2017, 'New insights into the heterogeneous ripening in Hass avocado via LC–MS/MS proteomics', Postharvest Biology and Technology, vol. 132, pp. 51-61. https://doi.org/10.1016/j.postharvbio.2017.06.001

New insights into the heterogeneous ripening in Hass avocado via LC–MS/MS proteomics. / Fuentealba, Claudia; Hernández, Ignacia; Olaeta, J. A.; Defilippi, Bruno; Meneses, Claudio; Campos, Reinaldo; Lurie, Susan; Carpentier, Sebastien; Pedreschi, Romina.

En: Postharvest Biology and Technology, Vol. 132, 01.10.2017, p. 51-61.

Resultado de la investigación: Article

TY - JOUR

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AU - Fuentealba, Claudia

AU - Hernández, Ignacia

AU - Olaeta, J. A.

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AU - Meneses, Claudio

AU - Campos, Reinaldo

AU - Lurie, Susan

AU - Carpentier, Sebastien

AU - Pedreschi, Romina

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AB - Hass avocado postharvest ripening heterogeneity creates several logistics problems at destination markets. Although several approaches mainly based on targeted analysis have been undertaken to broaden our understanding of the potential causes of this postharvest ripening heterogeneity, still the secret is not yet unveiled. In this study biopsies from individual fruit displaying contrasting ripening behavior (slow vs fast) and subjected to different storage conditions (immediately after harvest, 30 d regular air at 5 °C and 30 d at 5 °C and 4 kPa O2 and 6 kPa CO2) were analyzed for targeted fatty acid analysis and cell wall enzyme activity combined with a more integrative approach based on gel free proteomics LC–MS/MS. The high throughput proteomics was capable of discriminating between slow and fast ripening avocado fruit. Proteins that were mainly less abundant in the slow ripening phenotype were annotated to correct protein folding, translation and de novo synthesis and higher abundant proteins were annotated to amino sugar and nucleotide metabolism, detoxification and stress response and proteolysis. Our study opens new insights into the causes of heterogeneous ripening and may provide new markers for avocado ripening at harvest and after prolonged storage.

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KW - Fatty acids

KW - Gel-free proteomics

KW - Heterogeneity

KW - Persea americana

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