New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates

Pathima Udompijitkul, Maryam Alnoman, Saeed Banawas, Daniel Paredes-Sabja, Mahfuzur R. Sarker

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

Clostridium perfringens spore germination plays a critical role in the pathogenesis of C.perfringens-associated food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases. Germination is initiated when bacterial spores sense specific nutrient germinants (such as amino acids) through germinant receptors (GRs). In this study, we aimed to identify and characterize amino acid germinants for spores of enterotoxigenic C.perfringens type A. The polar, uncharged amino acids at pH 6.0 efficiently induced germination of C.perfringens spores; l-asparagine, l-cysteine, l-serine, and l-threonine triggered germination of spores of most FP and NFB isolates; whereas, l-glutamine was a unique germinant for FP spores. For cysteine- or glutamine-induced germination, gerKC spores (spores of a gerKC mutant derivative of FP strain SM101) germinated to a significantly lower extent and released less DPA than wild type spores; however, a less defective germination phenotype was observed in gerAA or gerKB spores. The germination defects in gerKC spores were partially restored by complementing the gerKC mutant with a recombinant plasmid carrying wild-type gerKA- KC, indicating that GerKC is an essential GR protein. The gerKA, gerKC, and gerKB spores germinated significantly slower with l-serine and l-threonine than their parental strain, suggesting the requirement for these GR proteins for normal germination of C.perfringens spores. In summary, these results indicate that the polar, uncharged amino acids at pH 6.0 are effective germinants for spores of C.perfringens type A and that GerKC is the main GR protein for germination of spores of FP strain SM101 with l-cysteine, l-glutamine, and l-asparagine.

Idioma originalEnglish
Páginas (desde-hasta)24-33
Número de páginas10
PublicaciónFood Microbiology
Volumen44
DOI
EstadoPublished - 1 ene 2014

Huella dactilar

Clostridium perfringens A
Clostridium perfringens
Spores
spores
Germination
Amino Acids
amino acids
foodborne illness
spore germination
Foodborne Diseases
germination
glutamine
cysteine
receptors
Glutamine
asparagine
threonine
Cysteine
serine
Asparagine

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Citar esto

Udompijitkul, Pathima ; Alnoman, Maryam ; Banawas, Saeed ; Paredes-Sabja, Daniel ; Sarker, Mahfuzur R. / New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates. En: Food Microbiology. 2014 ; Vol. 44. pp. 24-33.
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title = "New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates",
abstract = "Clostridium perfringens spore germination plays a critical role in the pathogenesis of C.perfringens-associated food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases. Germination is initiated when bacterial spores sense specific nutrient germinants (such as amino acids) through germinant receptors (GRs). In this study, we aimed to identify and characterize amino acid germinants for spores of enterotoxigenic C.perfringens type A. The polar, uncharged amino acids at pH 6.0 efficiently induced germination of C.perfringens spores; l-asparagine, l-cysteine, l-serine, and l-threonine triggered germination of spores of most FP and NFB isolates; whereas, l-glutamine was a unique germinant for FP spores. For cysteine- or glutamine-induced germination, gerKC spores (spores of a gerKC mutant derivative of FP strain SM101) germinated to a significantly lower extent and released less DPA than wild type spores; however, a less defective germination phenotype was observed in gerAA or gerKB spores. The germination defects in gerKC spores were partially restored by complementing the gerKC mutant with a recombinant plasmid carrying wild-type gerKA- KC, indicating that GerKC is an essential GR protein. The gerKA, gerKC, and gerKB spores germinated significantly slower with l-serine and l-threonine than their parental strain, suggesting the requirement for these GR proteins for normal germination of C.perfringens spores. In summary, these results indicate that the polar, uncharged amino acids at pH 6.0 are effective germinants for spores of C.perfringens type A and that GerKC is the main GR protein for germination of spores of FP strain SM101 with l-cysteine, l-glutamine, and l-asparagine.",
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New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates. / Udompijitkul, Pathima; Alnoman, Maryam; Banawas, Saeed; Paredes-Sabja, Daniel; Sarker, Mahfuzur R.

En: Food Microbiology, Vol. 44, 01.01.2014, p. 24-33.

Resultado de la investigación: Article

TY - JOUR

T1 - New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates

AU - Udompijitkul, Pathima

AU - Alnoman, Maryam

AU - Banawas, Saeed

AU - Paredes-Sabja, Daniel

AU - Sarker, Mahfuzur R.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Clostridium perfringens spore germination plays a critical role in the pathogenesis of C.perfringens-associated food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases. Germination is initiated when bacterial spores sense specific nutrient germinants (such as amino acids) through germinant receptors (GRs). In this study, we aimed to identify and characterize amino acid germinants for spores of enterotoxigenic C.perfringens type A. The polar, uncharged amino acids at pH 6.0 efficiently induced germination of C.perfringens spores; l-asparagine, l-cysteine, l-serine, and l-threonine triggered germination of spores of most FP and NFB isolates; whereas, l-glutamine was a unique germinant for FP spores. For cysteine- or glutamine-induced germination, gerKC spores (spores of a gerKC mutant derivative of FP strain SM101) germinated to a significantly lower extent and released less DPA than wild type spores; however, a less defective germination phenotype was observed in gerAA or gerKB spores. The germination defects in gerKC spores were partially restored by complementing the gerKC mutant with a recombinant plasmid carrying wild-type gerKA- KC, indicating that GerKC is an essential GR protein. The gerKA, gerKC, and gerKB spores germinated significantly slower with l-serine and l-threonine than their parental strain, suggesting the requirement for these GR proteins for normal germination of C.perfringens spores. In summary, these results indicate that the polar, uncharged amino acids at pH 6.0 are effective germinants for spores of C.perfringens type A and that GerKC is the main GR protein for germination of spores of FP strain SM101 with l-cysteine, l-glutamine, and l-asparagine.

AB - Clostridium perfringens spore germination plays a critical role in the pathogenesis of C.perfringens-associated food poisoning (FP) and non-food-borne (NFB) gastrointestinal diseases. Germination is initiated when bacterial spores sense specific nutrient germinants (such as amino acids) through germinant receptors (GRs). In this study, we aimed to identify and characterize amino acid germinants for spores of enterotoxigenic C.perfringens type A. The polar, uncharged amino acids at pH 6.0 efficiently induced germination of C.perfringens spores; l-asparagine, l-cysteine, l-serine, and l-threonine triggered germination of spores of most FP and NFB isolates; whereas, l-glutamine was a unique germinant for FP spores. For cysteine- or glutamine-induced germination, gerKC spores (spores of a gerKC mutant derivative of FP strain SM101) germinated to a significantly lower extent and released less DPA than wild type spores; however, a less defective germination phenotype was observed in gerAA or gerKB spores. The germination defects in gerKC spores were partially restored by complementing the gerKC mutant with a recombinant plasmid carrying wild-type gerKA- KC, indicating that GerKC is an essential GR protein. The gerKA, gerKC, and gerKB spores germinated significantly slower with l-serine and l-threonine than their parental strain, suggesting the requirement for these GR proteins for normal germination of C.perfringens spores. In summary, these results indicate that the polar, uncharged amino acids at pH 6.0 are effective germinants for spores of C.perfringens type A and that GerKC is the main GR protein for germination of spores of FP strain SM101 with l-cysteine, l-glutamine, and l-asparagine.

KW - Amino acids

KW - Clostridium perfringens

KW - Germinant receptors

KW - Germinants

KW - Spores

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DO - 10.1016/j.fm.2014.04.011

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