Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

Luis F. González, Francisca Henríquez-Belmar, Claudia Delgado-Acevedo, Marisol Cisternas-Olmedo, Gloria Arriagada, Ramón Sotomayor-Zárate, Dennis L. Murphy, Pablo R. Moya

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.

RESULTS: Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.

CONCLUSIONS: Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.

Idioma originalEnglish
Número de páginas1
PublicaciónBiological Research
Volumen50
N.º1
DOI
EstadoPublished - 19 sep 2017

Huella dactilar

obsessive-compulsive disorder
Amino Acid Transport System X-AG
Obsessive-Compulsive Disorder
Amphetamine
Locomotion
anxiety
glutamates
Compulsive Behavior
locomotion
amphetamine
Neurotransmitter Agents
transporters
Anxiety
Tissue
neurotransmitters
Calcium Carbonate
mice
High performance liquid chromatography
gamma-Aminobutyric Acid
Gene Components

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

González, L. F., Henríquez-Belmar, F., Delgado-Acevedo, C., Cisternas-Olmedo, M., Arriagada, G., Sotomayor-Zárate, R., ... Moya, P. R. (2017). Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. Biological Research, 50(1). https://doi.org/10.1186/s40659-017-0138-3
González, Luis F. ; Henríquez-Belmar, Francisca ; Delgado-Acevedo, Claudia ; Cisternas-Olmedo, Marisol ; Arriagada, Gloria ; Sotomayor-Zárate, Ramón ; Murphy, Dennis L. ; Moya, Pablo R. / Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. En: Biological Research. 2017 ; Vol. 50, N.º 1.
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abstract = "BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3{\%} of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.RESULTS: Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.CONCLUSIONS: Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.",
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González, LF, Henríquez-Belmar, F, Delgado-Acevedo, C, Cisternas-Olmedo, M, Arriagada, G, Sotomayor-Zárate, R, Murphy, DL & Moya, PR 2017, 'Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice', Biological Research, vol. 50, n.º 1. https://doi.org/10.1186/s40659-017-0138-3

Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. / González, Luis F.; Henríquez-Belmar, Francisca; Delgado-Acevedo, Claudia; Cisternas-Olmedo, Marisol; Arriagada, Gloria; Sotomayor-Zárate, Ramón; Murphy, Dennis L.; Moya, Pablo R.

En: Biological Research, Vol. 50, N.º 1, 19.09.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice

AU - González, Luis F.

AU - Henríquez-Belmar, Francisca

AU - Delgado-Acevedo, Claudia

AU - Cisternas-Olmedo, Marisol

AU - Arriagada, Gloria

AU - Sotomayor-Zárate, Ramón

AU - Murphy, Dennis L.

AU - Moya, Pablo R.

PY - 2017/9/19

Y1 - 2017/9/19

N2 - BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.RESULTS: Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.CONCLUSIONS: Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.

AB - BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.RESULTS: Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.CONCLUSIONS: Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.

KW - EAAT3

KW - Neuronal glutamate transporter

KW - Obsessive–compulsive disorder

KW - SLC1A1

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U2 - 10.1186/s40659-017-0138-3

DO - 10.1186/s40659-017-0138-3

M3 - Article

VL - 50

JO - Biological Research

JF - Biological Research

SN - 0716-9760

IS - 1

ER -

González LF, Henríquez-Belmar F, Delgado-Acevedo C, Cisternas-Olmedo M, Arriagada G, Sotomayor-Zárate R y otros. Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. Biological Research. 2017 sep 19;50(1). https://doi.org/10.1186/s40659-017-0138-3