Neisseria gonorrhoeae challenge increases matrix metalloproteinase-8 expression in fallopian tube explants

Natalia E. Juica, Paula I. Rodas, Paula Solar, Paula Borda, Renato Vargas, Cristobal Muñoz, Rodolfo Paredes, Myron Christodoulides, Luis A. Velasquez

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Background: Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections. The ECM is regulated by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), their endogenous inhibitors; MMP deregulation causes pathological conditions in a variety of tissues. Results: The aim of this work was to analyze the expression and localization of MMP-3, MMP-8, MMP-9, and TIMP-1 in FT explants during Ngo infection using real-time PCR, immunohistochemistry, zymography and ELISA. No significant variations in MMP-3, MMP-9, and TIMP-1 transcript levels were observed. In contrast, a significant increase (p < 0.05) was observed for MMP-8 expression and was accompanied by stromal immunoreactivity in infected explants. ELISA results supported these findings and showed that MMP-8 release increased upon gonococcal infection. Conclusions: Our results indicate that gonococcal infection induces increased MMP-8 expression, which might contribute to FT damage during infection.

Idioma originalEnglish
Número de artículo399
PublicaciónFrontiers in cellular and infection microbiology
Volumen7
N.ºSEP
DOI
EstadoPublished - 6 sep 2017

Huella dactilar

Matrix Metalloproteinase 8
Fallopian Tubes
Neisseria gonorrhoeae
Matrix Metalloproteinase Inhibitors
Extracellular Matrix
Infection
Matrix Metalloproteinase 3
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 9
Enzyme-Linked Immunosorbent Assay
Salpingitis
Tissue Inhibitor of Metalloproteinases
Gonorrhea
Sexually Transmitted Diseases
Matrix Metalloproteinases
Infertility
Cell Movement
Real-Time Polymerase Chain Reaction
Cell Differentiation
Immunohistochemistry

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Citar esto

Juica, Natalia E. ; Rodas, Paula I. ; Solar, Paula ; Borda, Paula ; Vargas, Renato ; Muñoz, Cristobal ; Paredes, Rodolfo ; Christodoulides, Myron ; Velasquez, Luis A. / Neisseria gonorrhoeae challenge increases matrix metalloproteinase-8 expression in fallopian tube explants. En: Frontiers in cellular and infection microbiology. 2017 ; Vol. 7, N.º SEP.
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title = "Neisseria gonorrhoeae challenge increases matrix metalloproteinase-8 expression in fallopian tube explants",
abstract = "Background: Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections. The ECM is regulated by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), their endogenous inhibitors; MMP deregulation causes pathological conditions in a variety of tissues. Results: The aim of this work was to analyze the expression and localization of MMP-3, MMP-8, MMP-9, and TIMP-1 in FT explants during Ngo infection using real-time PCR, immunohistochemistry, zymography and ELISA. No significant variations in MMP-3, MMP-9, and TIMP-1 transcript levels were observed. In contrast, a significant increase (p < 0.05) was observed for MMP-8 expression and was accompanied by stromal immunoreactivity in infected explants. ELISA results supported these findings and showed that MMP-8 release increased upon gonococcal infection. Conclusions: Our results indicate that gonococcal infection induces increased MMP-8 expression, which might contribute to FT damage during infection.",
keywords = "Extracellular matrix, Fallopian tubes, Metalloproteinases, Mucosal damage, Neisseria gonorrhoeae",
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Neisseria gonorrhoeae challenge increases matrix metalloproteinase-8 expression in fallopian tube explants. / Juica, Natalia E.; Rodas, Paula I.; Solar, Paula; Borda, Paula; Vargas, Renato; Muñoz, Cristobal; Paredes, Rodolfo; Christodoulides, Myron; Velasquez, Luis A.

En: Frontiers in cellular and infection microbiology, Vol. 7, N.º SEP, 399, 06.09.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Neisseria gonorrhoeae challenge increases matrix metalloproteinase-8 expression in fallopian tube explants

AU - Juica, Natalia E.

AU - Rodas, Paula I.

AU - Solar, Paula

AU - Borda, Paula

AU - Vargas, Renato

AU - Muñoz, Cristobal

AU - Paredes, Rodolfo

AU - Christodoulides, Myron

AU - Velasquez, Luis A.

PY - 2017/9/6

Y1 - 2017/9/6

N2 - Background: Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections. The ECM is regulated by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), their endogenous inhibitors; MMP deregulation causes pathological conditions in a variety of tissues. Results: The aim of this work was to analyze the expression and localization of MMP-3, MMP-8, MMP-9, and TIMP-1 in FT explants during Ngo infection using real-time PCR, immunohistochemistry, zymography and ELISA. No significant variations in MMP-3, MMP-9, and TIMP-1 transcript levels were observed. In contrast, a significant increase (p < 0.05) was observed for MMP-8 expression and was accompanied by stromal immunoreactivity in infected explants. ELISA results supported these findings and showed that MMP-8 release increased upon gonococcal infection. Conclusions: Our results indicate that gonococcal infection induces increased MMP-8 expression, which might contribute to FT damage during infection.

AB - Background: Neisseria gonorrhoeae (Ngo) is the etiological agent of gonorrhea, a sexually transmitted infection that initially infects the female lower genital tract. In untreated women, the bacteria can ascend to the upper genital reproductive tract and infect the fallopian tube (FTs), which is associated with salpingitis and can lead to impaired FT function and infertility. The extracellular matrix (ECM) plays an important role in cell migration and differentiation in the female genital tract, and some pathogens modify the ECM to establish successful infections. The ECM is regulated by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), their endogenous inhibitors; MMP deregulation causes pathological conditions in a variety of tissues. Results: The aim of this work was to analyze the expression and localization of MMP-3, MMP-8, MMP-9, and TIMP-1 in FT explants during Ngo infection using real-time PCR, immunohistochemistry, zymography and ELISA. No significant variations in MMP-3, MMP-9, and TIMP-1 transcript levels were observed. In contrast, a significant increase (p < 0.05) was observed for MMP-8 expression and was accompanied by stromal immunoreactivity in infected explants. ELISA results supported these findings and showed that MMP-8 release increased upon gonococcal infection. Conclusions: Our results indicate that gonococcal infection induces increased MMP-8 expression, which might contribute to FT damage during infection.

KW - Extracellular matrix

KW - Fallopian tubes

KW - Metalloproteinases

KW - Mucosal damage

KW - Neisseria gonorrhoeae

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U2 - 10.3389/fcimb.2017.00399

DO - 10.3389/fcimb.2017.00399

M3 - Article

AN - SCOPUS:85031495721

VL - 7

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

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ER -