Near infrared light induces post-translational modifications of human red blood cell proteins

Tomasz Walski, Agnieszka Dyrda, Małgorzata Dzik, Ludmiła Chludzińska, Tomasz Tomków, Joanna Mehl, Jerzy Detyna, Katarzyna Gałecka, Wojciech Witkiewicz, Małgorzata Komorowska

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

There is a growing body of evidence that near infrared (NIR) light exerts beneficial effects on cells. Its usefulness in the treatment of cancer, acute brain injuries, strokes and neurodegenerative disorders has been proposed. The mechanism of the NIR action is probably of photochemical nature, however it is not fully understood. Here, using a relatively simple biological model, human red blood cells (RBCs), and a polychromatic non-polarized light source, we investigate the impact of NIR radiation on the oxygen carrier, hemoglobin (Hb), and anion exchanger (AE1, Band 3). The exposure of intact RBCs to NIR light causes quaternary transitions in Hb, dehydration of proteins and decreases the amount of physiologically inactive methemoglobin, as detected by Raman spectroscopy. These effects are accompanied by a lowering of the intracellular pH (pHi) and changes in the cell membrane topography, as documented by atomic force microscopy (AFM). All those changes are in line with our previous studies where alterations of the membrane fluidity and membrane potential were attributed to NIR action on RBCs. The rate of the above listed changes depends strictly on the dose of NIR light that the cells receive, nonetheless it should not be considered as a thermal effect.

Idioma originalEnglish
Páginas (desde-hasta)2035-2045
Número de páginas11
PublicaciónPhotochemical and Photobiological Sciences
Volumen14
N.º11
DOI
EstadoPublished - 1 ene 2015
Publicado de forma externa

Huella dactilar

erythrocytes
Blood
Cells
hemoglobin
Infrared radiation
proteins
near infrared radiation
brain damage
membranes
bionics
Proteins
exchangers
strokes
cells
dehydration
temperature effects
topography
light sources
Hemoglobins
Raman spectroscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Citar esto

Walski, T., Dyrda, A., Dzik, M., Chludzińska, L., Tomków, T., Mehl, J., ... Komorowska, M. (2015). Near infrared light induces post-translational modifications of human red blood cell proteins. Photochemical and Photobiological Sciences, 14(11), 2035-2045. https://doi.org/10.1039/c5pp00203f
Walski, Tomasz ; Dyrda, Agnieszka ; Dzik, Małgorzata ; Chludzińska, Ludmiła ; Tomków, Tomasz ; Mehl, Joanna ; Detyna, Jerzy ; Gałecka, Katarzyna ; Witkiewicz, Wojciech ; Komorowska, Małgorzata. / Near infrared light induces post-translational modifications of human red blood cell proteins. En: Photochemical and Photobiological Sciences. 2015 ; Vol. 14, N.º 11. pp. 2035-2045.
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abstract = "There is a growing body of evidence that near infrared (NIR) light exerts beneficial effects on cells. Its usefulness in the treatment of cancer, acute brain injuries, strokes and neurodegenerative disorders has been proposed. The mechanism of the NIR action is probably of photochemical nature, however it is not fully understood. Here, using a relatively simple biological model, human red blood cells (RBCs), and a polychromatic non-polarized light source, we investigate the impact of NIR radiation on the oxygen carrier, hemoglobin (Hb), and anion exchanger (AE1, Band 3). The exposure of intact RBCs to NIR light causes quaternary transitions in Hb, dehydration of proteins and decreases the amount of physiologically inactive methemoglobin, as detected by Raman spectroscopy. These effects are accompanied by a lowering of the intracellular pH (pHi) and changes in the cell membrane topography, as documented by atomic force microscopy (AFM). All those changes are in line with our previous studies where alterations of the membrane fluidity and membrane potential were attributed to NIR action on RBCs. The rate of the above listed changes depends strictly on the dose of NIR light that the cells receive, nonetheless it should not be considered as a thermal effect.",
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Walski, T, Dyrda, A, Dzik, M, Chludzińska, L, Tomków, T, Mehl, J, Detyna, J, Gałecka, K, Witkiewicz, W & Komorowska, M 2015, 'Near infrared light induces post-translational modifications of human red blood cell proteins', Photochemical and Photobiological Sciences, vol. 14, n.º 11, pp. 2035-2045. https://doi.org/10.1039/c5pp00203f

Near infrared light induces post-translational modifications of human red blood cell proteins. / Walski, Tomasz; Dyrda, Agnieszka; Dzik, Małgorzata; Chludzińska, Ludmiła; Tomków, Tomasz; Mehl, Joanna; Detyna, Jerzy; Gałecka, Katarzyna; Witkiewicz, Wojciech; Komorowska, Małgorzata.

En: Photochemical and Photobiological Sciences, Vol. 14, N.º 11, 01.01.2015, p. 2035-2045.

Resultado de la investigación: Article

TY - JOUR

T1 - Near infrared light induces post-translational modifications of human red blood cell proteins

AU - Walski, Tomasz

AU - Dyrda, Agnieszka

AU - Dzik, Małgorzata

AU - Chludzińska, Ludmiła

AU - Tomków, Tomasz

AU - Mehl, Joanna

AU - Detyna, Jerzy

AU - Gałecka, Katarzyna

AU - Witkiewicz, Wojciech

AU - Komorowska, Małgorzata

PY - 2015/1/1

Y1 - 2015/1/1

N2 - There is a growing body of evidence that near infrared (NIR) light exerts beneficial effects on cells. Its usefulness in the treatment of cancer, acute brain injuries, strokes and neurodegenerative disorders has been proposed. The mechanism of the NIR action is probably of photochemical nature, however it is not fully understood. Here, using a relatively simple biological model, human red blood cells (RBCs), and a polychromatic non-polarized light source, we investigate the impact of NIR radiation on the oxygen carrier, hemoglobin (Hb), and anion exchanger (AE1, Band 3). The exposure of intact RBCs to NIR light causes quaternary transitions in Hb, dehydration of proteins and decreases the amount of physiologically inactive methemoglobin, as detected by Raman spectroscopy. These effects are accompanied by a lowering of the intracellular pH (pHi) and changes in the cell membrane topography, as documented by atomic force microscopy (AFM). All those changes are in line with our previous studies where alterations of the membrane fluidity and membrane potential were attributed to NIR action on RBCs. The rate of the above listed changes depends strictly on the dose of NIR light that the cells receive, nonetheless it should not be considered as a thermal effect.

AB - There is a growing body of evidence that near infrared (NIR) light exerts beneficial effects on cells. Its usefulness in the treatment of cancer, acute brain injuries, strokes and neurodegenerative disorders has been proposed. The mechanism of the NIR action is probably of photochemical nature, however it is not fully understood. Here, using a relatively simple biological model, human red blood cells (RBCs), and a polychromatic non-polarized light source, we investigate the impact of NIR radiation on the oxygen carrier, hemoglobin (Hb), and anion exchanger (AE1, Band 3). The exposure of intact RBCs to NIR light causes quaternary transitions in Hb, dehydration of proteins and decreases the amount of physiologically inactive methemoglobin, as detected by Raman spectroscopy. These effects are accompanied by a lowering of the intracellular pH (pHi) and changes in the cell membrane topography, as documented by atomic force microscopy (AFM). All those changes are in line with our previous studies where alterations of the membrane fluidity and membrane potential were attributed to NIR action on RBCs. The rate of the above listed changes depends strictly on the dose of NIR light that the cells receive, nonetheless it should not be considered as a thermal effect.

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U2 - 10.1039/c5pp00203f

DO - 10.1039/c5pp00203f

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VL - 14

SP - 2035

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JO - Photochemical and Photobiological Sciences

JF - Photochemical and Photobiological Sciences

SN - 1474-905X

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