Natural programmed cell death in T. cruzi epimastigotes maintained in axenic cultures

V. Jimenez, R. Paredes, M. A. Sosa, N. Galanti

Resultado de la investigación: Article

45 Citas (Scopus)

Resumen

Trypanosoma cruzi, a parasitic protozoan, is the agent of Chagas' disease or American trypanosomiasis, an endemic pathology in Latin America, affecting up to 18 million people, with high public health costs. Programmed cell death (PCD) has many functions in development and tissue remodeling in metazoans. In protozoa, it could represent concomitant or alternative mechanisms for clonal selection, immune response evasion, and population size regulation. In this work, we describe the natural occurrence of PCD in T. cruzi epimastigotes during the stationary phase of growth in axenic culture or under nutrient deprivation. Thus, we have observed phosphatidylserine externalization, cellular volume decrease, caspase-like protein activity, and DNA fragmentation. Additionally, serum deprivation also induces autophagic characteristics such as monodansylcadaverine-labeled vesicles accumulation and redistribution of proteins homologous to Atg8. In agreement with our results, apoptosis may play an important role in parasite survival. Then, identification and modulation of molecular targets inducing programmed cell death in T. cruzi may lead to new potential therapeutic approaches for Chagas' disease.

Idioma originalEnglish
Páginas (desde-hasta)688-698
Número de páginas11
PublicaciónJournal of Cellular Biochemistry
Volumen105
N.º3
DOI
EstadoPublished - 15 oct 2008

Huella dactilar

Axenic Culture
Chagas Disease
Trypanosoma cruzi
Cell death
Cell Death
Protozoa
Immune Evasion
Latin America
Phosphatidylserines
Public health
Pathology
DNA Fragmentation
Caspases
Population Density
Health Care Costs
Nutrients
Parasites
Proteins
Public Health
Modulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

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Natural programmed cell death in T. cruzi epimastigotes maintained in axenic cultures. / Jimenez, V.; Paredes, R.; Sosa, M. A.; Galanti, N.

En: Journal of Cellular Biochemistry, Vol. 105, N.º 3, 15.10.2008, p. 688-698.

Resultado de la investigación: Article

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AU - Sosa, M. A.

AU - Galanti, N.

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