Natural programmed cell death in T. cruzi epimastigotes maintained in axenic cultures

V. Jimenez, R. Paredes, M. A. Sosa, N. Galanti

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

58 Citas (Scopus)


Trypanosoma cruzi, a parasitic protozoan, is the agent of Chagas' disease or American trypanosomiasis, an endemic pathology in Latin America, affecting up to 18 million people, with high public health costs. Programmed cell death (PCD) has many functions in development and tissue remodeling in metazoans. In protozoa, it could represent concomitant or alternative mechanisms for clonal selection, immune response evasion, and population size regulation. In this work, we describe the natural occurrence of PCD in T. cruzi epimastigotes during the stationary phase of growth in axenic culture or under nutrient deprivation. Thus, we have observed phosphatidylserine externalization, cellular volume decrease, caspase-like protein activity, and DNA fragmentation. Additionally, serum deprivation also induces autophagic characteristics such as monodansylcadaverine-labeled vesicles accumulation and redistribution of proteins homologous to Atg8. In agreement with our results, apoptosis may play an important role in parasite survival. Then, identification and modulation of molecular targets inducing programmed cell death in T. cruzi may lead to new potential therapeutic approaches for Chagas' disease.

Idioma originalInglés
Páginas (desde-hasta)688-698
Número de páginas11
PublicaciónJournal of Cellular Biochemistry
EstadoPublicada - 15 oct. 2008

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular


Profundice en los temas de investigación de 'Natural programmed cell death in T. cruzi epimastigotes maintained in axenic cultures'. En conjunto forman una huella única.

Citar esto