Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers

Pedro Labarca, Sergio Lobos, Inés Calderón, Guido Mora

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.

Idioma originalEnglish
Páginas (desde-hasta)211-216
Número de páginas6
PublicaciónFEBS Letters
Volumen197
N.º1-2
DOI
EstadoPublished - 3 mar 1986

Huella dactilar

Porins
Salmonella typhi
Salmonella
Lipid bilayers
Lipid Bilayers
Pyridoxal Phosphate
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Citar esto

Labarca, Pedro ; Lobos, Sergio ; Calderón, Inés ; Mora, Guido. / Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers. En: FEBS Letters. 1986 ; Vol. 197, N.º 1-2. pp. 211-216.
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abstract = "Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.",
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Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers. / Labarca, Pedro; Lobos, Sergio; Calderón, Inés; Mora, Guido.

En: FEBS Letters, Vol. 197, N.º 1-2, 03.03.1986, p. 211-216.

Resultado de la investigación: Article

TY - JOUR

T1 - Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers

AU - Labarca, Pedro

AU - Lobos, Sergio

AU - Calderón, Inés

AU - Mora, Guido

PY - 1986/3/3

Y1 - 1986/3/3

N2 - Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.

AB - Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.

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