NAD(P)H Oxidase-derived H2O2 Signals Chloride Channel Activation in Cell Volume Regulation and Cell Proliferation

Diego Varela, Felipe Simon, Ana Riveros, Finn Jørgensen, Andrés Stutzin

Resultado de la investigación: Article

115 Citas (Scopus)

Resumen

Cellular swelling triggers the activation of Cl- channels (volume-sensitive outwardly rectifying (VSOR) Cl- channels) in many cell types. Ensuing regulatory volume decrease has been considered the primary function of these channels. However, Cl- channels, which share functional properties with volume-sensitive Cl- channels, have been shown to be involved in other physiological processes, including cell proliferation and apoptosis, raising the question of their physiological roles and the signal transduction pathways involved in their activation. Here we report that exogenously applied H2O2 elicited VSOR Cl - channel activation. Furthermore, activation of these channels was found to be coupled to NAD(P)H oxidase activity. Also, epidermal growth factor, known to increase H2O2 production, activated Cl - channels with properties identical to swelling-sensitive Cl - channels. It is concluded that NAD(P)H oxidase-derived H 2O2 is the common signal transducing molecule that mediates the activation of these ubiquitously expressed anion channels under a variety of physiological conditions.

Idioma originalEnglish
Páginas (desde-hasta)13301-13304
Número de páginas4
PublicaciónJournal of Biological Chemistry
Volumen279
N.º14
DOI
EstadoPublished - 2 abr 2004

Huella dactilar

Chloride Channels
NADPH Oxidase
Cell proliferation
Cell Size
Chemical activation
Cell Proliferation
Physiological Phenomena
Epidermal Growth Factor
Anions
Signal Transduction
Swelling
Apoptosis
Signal transduction
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Citar esto

Varela, Diego ; Simon, Felipe ; Riveros, Ana ; Jørgensen, Finn ; Stutzin, Andrés. / NAD(P)H Oxidase-derived H2O2 Signals Chloride Channel Activation in Cell Volume Regulation and Cell Proliferation. En: Journal of Biological Chemistry. 2004 ; Vol. 279, N.º 14. pp. 13301-13304.
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abstract = "Cellular swelling triggers the activation of Cl- channels (volume-sensitive outwardly rectifying (VSOR) Cl- channels) in many cell types. Ensuing regulatory volume decrease has been considered the primary function of these channels. However, Cl- channels, which share functional properties with volume-sensitive Cl- channels, have been shown to be involved in other physiological processes, including cell proliferation and apoptosis, raising the question of their physiological roles and the signal transduction pathways involved in their activation. Here we report that exogenously applied H2O2 elicited VSOR Cl - channel activation. Furthermore, activation of these channels was found to be coupled to NAD(P)H oxidase activity. Also, epidermal growth factor, known to increase H2O2 production, activated Cl - channels with properties identical to swelling-sensitive Cl - channels. It is concluded that NAD(P)H oxidase-derived H 2O2 is the common signal transducing molecule that mediates the activation of these ubiquitously expressed anion channels under a variety of physiological conditions.",
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NAD(P)H Oxidase-derived H2O2 Signals Chloride Channel Activation in Cell Volume Regulation and Cell Proliferation. / Varela, Diego; Simon, Felipe; Riveros, Ana; Jørgensen, Finn; Stutzin, Andrés.

En: Journal of Biological Chemistry, Vol. 279, N.º 14, 02.04.2004, p. 13301-13304.

Resultado de la investigación: Article

TY - JOUR

T1 - NAD(P)H Oxidase-derived H2O2 Signals Chloride Channel Activation in Cell Volume Regulation and Cell Proliferation

AU - Varela, Diego

AU - Simon, Felipe

AU - Riveros, Ana

AU - Jørgensen, Finn

AU - Stutzin, Andrés

PY - 2004/4/2

Y1 - 2004/4/2

N2 - Cellular swelling triggers the activation of Cl- channels (volume-sensitive outwardly rectifying (VSOR) Cl- channels) in many cell types. Ensuing regulatory volume decrease has been considered the primary function of these channels. However, Cl- channels, which share functional properties with volume-sensitive Cl- channels, have been shown to be involved in other physiological processes, including cell proliferation and apoptosis, raising the question of their physiological roles and the signal transduction pathways involved in their activation. Here we report that exogenously applied H2O2 elicited VSOR Cl - channel activation. Furthermore, activation of these channels was found to be coupled to NAD(P)H oxidase activity. Also, epidermal growth factor, known to increase H2O2 production, activated Cl - channels with properties identical to swelling-sensitive Cl - channels. It is concluded that NAD(P)H oxidase-derived H 2O2 is the common signal transducing molecule that mediates the activation of these ubiquitously expressed anion channels under a variety of physiological conditions.

AB - Cellular swelling triggers the activation of Cl- channels (volume-sensitive outwardly rectifying (VSOR) Cl- channels) in many cell types. Ensuing regulatory volume decrease has been considered the primary function of these channels. However, Cl- channels, which share functional properties with volume-sensitive Cl- channels, have been shown to be involved in other physiological processes, including cell proliferation and apoptosis, raising the question of their physiological roles and the signal transduction pathways involved in their activation. Here we report that exogenously applied H2O2 elicited VSOR Cl - channel activation. Furthermore, activation of these channels was found to be coupled to NAD(P)H oxidase activity. Also, epidermal growth factor, known to increase H2O2 production, activated Cl - channels with properties identical to swelling-sensitive Cl - channels. It is concluded that NAD(P)H oxidase-derived H 2O2 is the common signal transducing molecule that mediates the activation of these ubiquitously expressed anion channels under a variety of physiological conditions.

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