Mutaciones del gen supresor de tumor p53 en adenocarcinoma gástrico

Marcela Aranda L, Neptalí Náquira V, Rafael Karque O, Hegaly Mendoza V, Carolina Sepúlveda T, Claudio Silva Z

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

Background: We have shown numeric alterations such as byperploidy and bypoploidy with loss of chromosome 17 in primary gastric cancer. This chromosome maps p53 suppressor gene that induces the transcription of genes related to cellular cycle control, DNA synthesis and repair, cellular differentiation and apoptosis. Aim: To analyze, at a molecular level, the possible alterations of p53 suppressor gene in samples of gastric cancer and non tumoral mucosa. Material and methods: Tissue samples of gastric carcinoma and non tumoral gastric mucosa coming from 26 patients subjected to a total gastrectomy were analyzed. The mutation of p53 suppressor gene exons 7 to 9 were determined using a conformational polymorphism analysis in single strands of the gene and indirect sequencing in some cases. Results: Alterations in p53 gene were found in 77% of tumoral and 19% of non tumoral samples. T insertions in codons 260, 317 and 321, G insertion in codon 328 and G-T transvertion in codon 302 were found. Aminoacid sequence analysis of p53 protein obtained with sequencing data showed that T insertion in codon 260 could translate three erroneous aminoacids after the mutation and produce a truncated protein due to the creation of a stop codon. No associations between alterations of p53 gene and clinical or pathological variables such as age, sex, tumor localization, histological type and presence of lymph node metastases were observed. Conclusions: Mutations of p53 suppressor gene are frequent in gastric carcinoma.

Título traducido de la contribuciónMutations of p53 suppressor gene in gastric adenocarcinoma
Idioma originalEspañol
Páginas (desde-hasta)525-532
Número de páginas8
PublicaciónRevista Medica de Chile
Volumen126
N.º5
EstadoPublicada - 1 may 1998

Palabras clave

  • Adenocarcinoma
  • Carcinoma
  • Chromosomas, human
  • Genes

Áreas temáticas de ASJC Scopus

  • Medicina (todo)

Huella Profundice en los temas de investigación de 'Mutaciones del gen supresor de tumor p53 en adenocarcinoma gástrico'. En conjunto forman una huella única.

Citar esto