Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure

Adolfo Isla, Mónica Saldarriaga-Córdoba, Derie E. Fuentes, Romina Albornoz, Denise Haussmann, Jorge Mancilla-Schulz, Alexis Martínez, Jaime Figueroa, Ruben Avendaño-Herrera, Alejandro Yáñez

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic-resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty-two Chilean P. salmonisisolates, as well as the type strain LF-89 T , were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF-89 T and EM-90 strains, as well as a seven-isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.

Idioma originalEnglish
Páginas (desde-hasta)721-737
Número de páginas17
PublicaciónJournal of Fish Diseases
Volumen42
N.º5
DOI
EstadoPublished - 1 may 2019

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
Multilocus Sequence Typing
Fisheries
fish farms
Oncorhynchus
population structure
Genotype
farm
Piscirickettsiaceae Infections
genetic variation
fish
pathogen
Population
Salmo salar
Aquaculture
aquaculture industry
loci
bioinformatics
antibiotic resistance

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Citar esto

Isla, A., Saldarriaga-Córdoba, M., Fuentes, D. E., Albornoz, R., Haussmann, D., Mancilla-Schulz, J., ... Yáñez, A. (2019). Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure. Journal of Fish Diseases, 42(5), 721-737. https://doi.org/10.1111/jfd.12976
Isla, Adolfo ; Saldarriaga-Córdoba, Mónica ; Fuentes, Derie E. ; Albornoz, Romina ; Haussmann, Denise ; Mancilla-Schulz, Jorge ; Martínez, Alexis ; Figueroa, Jaime ; Avendaño-Herrera, Ruben ; Yáñez, Alejandro. / Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms : Evidence for genetic diversity and population structure. En: Journal of Fish Diseases. 2019 ; Vol. 42, N.º 5. pp. 721-737.
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abstract = "Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic-resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty-two Chilean P. salmonisisolates, as well as the type strain LF-89 T , were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48{\%} of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF-89 T and EM-90 strains, as well as a seven-isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.",
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author = "Adolfo Isla and M{\'o}nica Saldarriaga-C{\'o}rdoba and Fuentes, {Derie E.} and Romina Albornoz and Denise Haussmann and Jorge Mancilla-Schulz and Alexis Mart{\'i}nez and Jaime Figueroa and Ruben Avenda{\~n}o-Herrera and Alejandro Y{\'a}{\~n}ez",
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Isla, A, Saldarriaga-Córdoba, M, Fuentes, DE, Albornoz, R, Haussmann, D, Mancilla-Schulz, J, Martínez, A, Figueroa, J, Avendaño-Herrera, R & Yáñez, A 2019, 'Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure', Journal of Fish Diseases, vol. 42, n.º 5, pp. 721-737. https://doi.org/10.1111/jfd.12976

Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms : Evidence for genetic diversity and population structure. / Isla, Adolfo; Saldarriaga-Córdoba, Mónica; Fuentes, Derie E.; Albornoz, Romina; Haussmann, Denise; Mancilla-Schulz, Jorge; Martínez, Alexis; Figueroa, Jaime; Avendaño-Herrera, Ruben; Yáñez, Alejandro.

En: Journal of Fish Diseases, Vol. 42, N.º 5, 01.05.2019, p. 721-737.

Resultado de la investigación: Article

TY - JOUR

T1 - Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms

T2 - Evidence for genetic diversity and population structure

AU - Isla, Adolfo

AU - Saldarriaga-Córdoba, Mónica

AU - Fuentes, Derie E.

AU - Albornoz, Romina

AU - Haussmann, Denise

AU - Mancilla-Schulz, Jorge

AU - Martínez, Alexis

AU - Figueroa, Jaime

AU - Avendaño-Herrera, Ruben

AU - Yáñez, Alejandro

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic-resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty-two Chilean P. salmonisisolates, as well as the type strain LF-89 T , were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF-89 T and EM-90 strains, as well as a seven-isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.

AB - Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic-resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty-two Chilean P. salmonisisolates, as well as the type strain LF-89 T , were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF-89 T and EM-90 strains, as well as a seven-isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.

KW - genetic evolution

KW - hybrid genogroup

KW - MLST scheme

KW - Piscirickettsia salmonis

KW - salmonid pathogen

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U2 - 10.1111/jfd.12976

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