Morphological, immunological and biochemical characterization of purified transverse tubule membranes isolated from rabbit skeletal muscle

Mario S. Rosemblatt, Donald J. Scales

Resultado de la investigación: Contribución a una revistaArtículo

12 Citas (Scopus)

Resumen

A microsomal fraction consisting of membranes of transverse tubule origin has been purified by a modification of the calcium-loading procedure initially described by Rosemblatt et al. (J Biol Chem 256:8140-8, 1981). Enzymatic analysis of this fraction shows an enrichment of the vesicles in the Mg++ATPase (basal) activity characteristic of the T-tubules and an absent or very low Ca++-dependant ATPase activity. Stereological analysis of freeze fracture replica of the membranes in the purified fraction indicates that they have a very low density of particles in their P faces and lack the structural manifestation of the caveolae typical of the sarcolemma. Immunological analysis performed with monoclonal antibodies prepared against purified T-tubule and sarcoplasmic reticulum membranes define some T-tubule specific antigens and confirm the morphological and biochemical data regarding the origin and purity of the Ttubule preparation.

Idioma originalInglés
Páginas (desde-hasta)57-69
Número de páginas13
PublicaciónMolecular and Cellular Biochemistry
Volumen87
N.º1
DOI
EstadoPublicada - may 1989

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Genética
  • Bioquímica clínica
  • Biología celular
  • Medicina (todo)

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