Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons

Felipe A. Court, Rajiv Midha, Bruno A. Cisterna, Joey Grochmal, Antos Shakhbazau, William T. Hendriks, Jan Van Minnen

Resultado de la investigación: Article

66 Citas (Scopus)

Resumen

Recently, we showed that Schwann cells transfer ribosomes to injured axons. Here, we demonstrate that Schwann cells transfer ribosomes to regenerating axons in vivo. For this, we used lentiviral vector-mediated expression of ribosomal protein L4 and eGFP to label ribosomes in Schwann cells. Two approaches were followed. First, we transduced Schwann cells in vivo in the distal trunk of the sciatic nerve after a nerve crush. Seven days after the crush, 12% of regenerating axons contained fluorescent ribosomes. Second, we transduced Schwann cells in vitro that were subsequently injected into an acellular nerve graft that was inserted into the sciatic nerve. Fluorescent ribosomes were detected in regenerating axons up to 8 weeks after graft insertion. Together, these data indicate that regenerating axons receive ribosomes from Schwann cells and, furthermore, that Schwann cells may support local axonal protein synthesis by transferring protein synthetic machinery and mRNAs to these axons.

Idioma originalEnglish
Páginas (desde-hasta)1529-1539
Número de páginas11
PublicaciónGLIA
Volumen59
N.º10
DOI
EstadoPublished - oct 2011

Huella dactilar

Schwann Cells
Ribosomes
Axons
Sciatic Nerve
Nerve Crush
Transplants
Proteins
Messenger RNA

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Citar esto

Court, F. A., Midha, R., Cisterna, B. A., Grochmal, J., Shakhbazau, A., Hendriks, W. T., & Van Minnen, J. (2011). Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons. GLIA, 59(10), 1529-1539. https://doi.org/10.1002/glia.21196
Court, Felipe A. ; Midha, Rajiv ; Cisterna, Bruno A. ; Grochmal, Joey ; Shakhbazau, Antos ; Hendriks, William T. ; Van Minnen, Jan. / Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons. En: GLIA. 2011 ; Vol. 59, N.º 10. pp. 1529-1539.
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Court, FA, Midha, R, Cisterna, BA, Grochmal, J, Shakhbazau, A, Hendriks, WT & Van Minnen, J 2011, 'Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons', GLIA, vol. 59, n.º 10, pp. 1529-1539. https://doi.org/10.1002/glia.21196

Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons. / Court, Felipe A.; Midha, Rajiv; Cisterna, Bruno A.; Grochmal, Joey; Shakhbazau, Antos; Hendriks, William T.; Van Minnen, Jan.

En: GLIA, Vol. 59, N.º 10, 10.2011, p. 1529-1539.

Resultado de la investigación: Article

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AU - Court, Felipe A.

AU - Midha, Rajiv

AU - Cisterna, Bruno A.

AU - Grochmal, Joey

AU - Shakhbazau, Antos

AU - Hendriks, William T.

AU - Van Minnen, Jan

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KW - Lentiviral transduction

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KW - Peripheral nervous system

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Court FA, Midha R, Cisterna BA, Grochmal J, Shakhbazau A, Hendriks WT y otros. Morphological evidence for a transport of ribosomes from Schwann cells to regenerating axons. GLIA. 2011 oct;59(10):1529-1539. https://doi.org/10.1002/glia.21196