Resumen
A technique is described for the identification and enumeration of bioleaching microorganisms that circumvents many of the classic problems. The technique is based on the utilization of cloned Thiobacillus ferrooxidans gene sequences as probes for determining genetic homologies using dot blot and Southern blot assays. The results can be quantitated, yielding data on the number as well as the identity of microorganisms. The technique may also prove useful for analyzing samples from bioleaching operations, evaluating the maintenance of genetically-engineered strains in a bioleaching operation and for classifying microorganisms according to their genetic, rather than phenotypic, similarities.
Idioma original | Inglés |
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Título de la publicación alojada | Biotechnology and Bioengineering Symposium |
Editorial | John Wiley & Sons |
Páginas | 301-309 |
Número de páginas | 9 |
Edición | 16 |
ISBN (versión impresa) | 0471851027 |
Estado | Publicada - 1986 |
Áreas temáticas de ASJC Scopus
- Bioquímica, Genética y Biología Molecular General