MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS.

James R. Yates, David S. Holmes

Resultado de la investigación: Conference contribution

4 Citas (Scopus)

Resumen

A technique is described for the identification and enumeration of bioleaching microorganisms that circumvents many of the classic problems. The technique is based on the utilization of cloned Thiobacillus ferrooxidans gene sequences as probes for determining genetic homologies using dot blot and Southern blot assays. The results can be quantitated, yielding data on the number as well as the identity of microorganisms. The technique may also prove useful for analyzing samples from bioleaching operations, evaluating the maintenance of genetically-engineered strains in a bioleaching operation and for classifying microorganisms according to their genetic, rather than phenotypic, similarities.

Idioma originalEnglish
Título de la publicación alojadaBiotechnology and Bioengineering Symposium
EditorialJohn Wiley & Sons
Páginas301-309
Número de páginas9
Edición16
ISBN (versión impresa)0471851027
EstadoPublished - 1986

Huella dactilar

Thiobacillus
Bioleaching
Molecular Probes
Tailings
Southern Blotting
Microorganisms
Maintenance
Genes
Assays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Citar esto

Yates, J. R., & Holmes, D. S. (1986). MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS. En Biotechnology and Bioengineering Symposium (16 ed., pp. 301-309). John Wiley & Sons.
Yates, James R. ; Holmes, David S. / MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS. Biotechnology and Bioengineering Symposium. 16. ed. John Wiley & Sons, 1986. pp. 301-309
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Yates, JR & Holmes, DS 1986, MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS. En Biotechnology and Bioengineering Symposium. 16 ed., John Wiley & Sons, pp. 301-309.

MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS. / Yates, James R.; Holmes, David S.

Biotechnology and Bioengineering Symposium. 16. ed. John Wiley & Sons, 1986. p. 301-309.

Resultado de la investigación: Conference contribution

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AU - Holmes, David S.

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AB - A technique is described for the identification and enumeration of bioleaching microorganisms that circumvents many of the classic problems. The technique is based on the utilization of cloned Thiobacillus ferrooxidans gene sequences as probes for determining genetic homologies using dot blot and Southern blot assays. The results can be quantitated, yielding data on the number as well as the identity of microorganisms. The technique may also prove useful for analyzing samples from bioleaching operations, evaluating the maintenance of genetically-engineered strains in a bioleaching operation and for classifying microorganisms according to their genetic, rather than phenotypic, similarities.

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Yates JR, Holmes DS. MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS. En Biotechnology and Bioengineering Symposium. 16 ed. John Wiley & Sons. 1986. p. 301-309