Molecular investigation of zoonotic intracellular bacteria in Chilean bats

Ananda Müller, Paulina Sepúlveda, Sophia Di Cataldo, Aitor Cevidanes, Fulgencio Lisón, Javier Millán

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

Intracellular pathogens were investigated for the first time in 55 Chilean bats belonging to six species. Using a conventional PCR protocol targeting a fragment of the ITS region, 21 bats (38 %) were positive for DNA of Bartonella sp. Molecular characterization of fragments of the gltA, rpoB and fstZ genes and subsequent phylogenetic analysis indicated the presence of diverse genotypes related to Bartonella from bats worldwide. DNA from C. burnetii was investigated using a real-time PCR (qPCR) protocol targeting the IS1111 gene and yielded positive results for 5 individuals (9%), being the first report of C. burnetii in wildlife in Chile. All bats were negative for Rickettsia sp., evaluated by qPCR for the gltA gene, confirming that bats do not act as important reservoirs for Rickettsia. This preliminary survey calls for more comprehensive studies on the epidemiology of these agents, including larger sample sizes, the evaluation of potential transmission routes and spillover potential.

Idioma originalInglés
Número de artículo101541
PublicaciónComparative Immunology, Microbiology and Infectious Diseases
Volumen73
DOI
EstadoPublicada - dic 2020

Áreas temáticas de ASJC Scopus

  • Inmulogía y alergología
  • Microbiología
  • Inmunología
  • Veterinaria (todo)
  • Enfermedades infecciosas

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