Molecular evidence of shared hookworm Ancylostoma tubaeforme haplotypes between the critically endangered Iberian lynx and sympatric domestic cats

Javier Millán, Isabel Blasco-Costa

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Hookworms of the genus Ancylostoma are the most pathogenic parasites of young cats, and A. tubaeforme may cause morbidity or mortality in young individuals of the most endangered felid species in the world, the Iberian lynx (Lynx pardinus). Since the transmission of monoxenous parasites is related to host density and remaining lynx populations are currently very small, the presence of reservoir hosts may be necessary for the maintenance of the hookworm life-cycle, the domestic cat being the most likely reservoir of A. tubaeforme. In order to confirm this hypothesis, the mitochondrial cytochrome c oxidase subunit I gene (Cox I) sequences of three A. tubaeforme specimens from a road-killed Iberian lynx from Doñana were compared with 14 specimens retrieved from five sympatric free-roaming cats from the same area, and with six specimens from three free-roaming cats from the Mediterranean island of Mallorca. Gene fragments (300. bp) from 23 A. tubaeforme individuals representing 16 different haplotypes were obtained. A statistical parsimony haplotype network analysis showed that the three specimens infecting an Iberian lynx corresponded to two different haplotypes, one of which was identical to a specimen in a cat found only 10. km from the lynx. Specimens from the Iberian lynx and those from cats in Doñana were only 1.03% genetically divergent, whereas specimens from Mallorca cats and those from Doñana cats and the lynx diverged by 1.33% and 1.36%, respectively. The existence of shared haplotypes of hookworms between lynx and cat reinforces the hypothesis that the abundant sympatric domestic cat population is acting as a reservoir for A. tubaeforme infection in the endangered Iberian lynx.

Idioma originalEnglish
Páginas (desde-hasta)518-522
Número de páginas5
PublicaciónVeterinary Parasitology
Volumen186
N.º3-4
DOI
EstadoPublished - 25 may 2012

Huella dactilar

Ancylostoma tubaeforme
Lynx
Ancylostoma
Ancylostomatoidea
hookworms
Haplotypes
haplotypes
Cats
cats
Mediterranean Islands
Parasites
Lynx pardinus
parasites
Endangered Species
disease reservoirs
Felidae
Electron Transport Complex IV
Life Cycle Stages
cytochrome-c oxidase
Population

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Citar esto

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title = "Molecular evidence of shared hookworm Ancylostoma tubaeforme haplotypes between the critically endangered Iberian lynx and sympatric domestic cats",
abstract = "Hookworms of the genus Ancylostoma are the most pathogenic parasites of young cats, and A. tubaeforme may cause morbidity or mortality in young individuals of the most endangered felid species in the world, the Iberian lynx (Lynx pardinus). Since the transmission of monoxenous parasites is related to host density and remaining lynx populations are currently very small, the presence of reservoir hosts may be necessary for the maintenance of the hookworm life-cycle, the domestic cat being the most likely reservoir of A. tubaeforme. In order to confirm this hypothesis, the mitochondrial cytochrome c oxidase subunit I gene (Cox I) sequences of three A. tubaeforme specimens from a road-killed Iberian lynx from Do{\~n}ana were compared with 14 specimens retrieved from five sympatric free-roaming cats from the same area, and with six specimens from three free-roaming cats from the Mediterranean island of Mallorca. Gene fragments (300. bp) from 23 A. tubaeforme individuals representing 16 different haplotypes were obtained. A statistical parsimony haplotype network analysis showed that the three specimens infecting an Iberian lynx corresponded to two different haplotypes, one of which was identical to a specimen in a cat found only 10. km from the lynx. Specimens from the Iberian lynx and those from cats in Do{\~n}ana were only 1.03{\%} genetically divergent, whereas specimens from Mallorca cats and those from Do{\~n}ana cats and the lynx diverged by 1.33{\%} and 1.36{\%}, respectively. The existence of shared haplotypes of hookworms between lynx and cat reinforces the hypothesis that the abundant sympatric domestic cat population is acting as a reservoir for A. tubaeforme infection in the endangered Iberian lynx.",
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Molecular evidence of shared hookworm Ancylostoma tubaeforme haplotypes between the critically endangered Iberian lynx and sympatric domestic cats. / Millán, Javier; Blasco-Costa, Isabel.

En: Veterinary Parasitology, Vol. 186, N.º 3-4, 25.05.2012, p. 518-522.

Resultado de la investigación: Article

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T1 - Molecular evidence of shared hookworm Ancylostoma tubaeforme haplotypes between the critically endangered Iberian lynx and sympatric domestic cats

AU - Millán, Javier

AU - Blasco-Costa, Isabel

PY - 2012/5/25

Y1 - 2012/5/25

N2 - Hookworms of the genus Ancylostoma are the most pathogenic parasites of young cats, and A. tubaeforme may cause morbidity or mortality in young individuals of the most endangered felid species in the world, the Iberian lynx (Lynx pardinus). Since the transmission of monoxenous parasites is related to host density and remaining lynx populations are currently very small, the presence of reservoir hosts may be necessary for the maintenance of the hookworm life-cycle, the domestic cat being the most likely reservoir of A. tubaeforme. In order to confirm this hypothesis, the mitochondrial cytochrome c oxidase subunit I gene (Cox I) sequences of three A. tubaeforme specimens from a road-killed Iberian lynx from Doñana were compared with 14 specimens retrieved from five sympatric free-roaming cats from the same area, and with six specimens from three free-roaming cats from the Mediterranean island of Mallorca. Gene fragments (300. bp) from 23 A. tubaeforme individuals representing 16 different haplotypes were obtained. A statistical parsimony haplotype network analysis showed that the three specimens infecting an Iberian lynx corresponded to two different haplotypes, one of which was identical to a specimen in a cat found only 10. km from the lynx. Specimens from the Iberian lynx and those from cats in Doñana were only 1.03% genetically divergent, whereas specimens from Mallorca cats and those from Doñana cats and the lynx diverged by 1.33% and 1.36%, respectively. The existence of shared haplotypes of hookworms between lynx and cat reinforces the hypothesis that the abundant sympatric domestic cat population is acting as a reservoir for A. tubaeforme infection in the endangered Iberian lynx.

AB - Hookworms of the genus Ancylostoma are the most pathogenic parasites of young cats, and A. tubaeforme may cause morbidity or mortality in young individuals of the most endangered felid species in the world, the Iberian lynx (Lynx pardinus). Since the transmission of monoxenous parasites is related to host density and remaining lynx populations are currently very small, the presence of reservoir hosts may be necessary for the maintenance of the hookworm life-cycle, the domestic cat being the most likely reservoir of A. tubaeforme. In order to confirm this hypothesis, the mitochondrial cytochrome c oxidase subunit I gene (Cox I) sequences of three A. tubaeforme specimens from a road-killed Iberian lynx from Doñana were compared with 14 specimens retrieved from five sympatric free-roaming cats from the same area, and with six specimens from three free-roaming cats from the Mediterranean island of Mallorca. Gene fragments (300. bp) from 23 A. tubaeforme individuals representing 16 different haplotypes were obtained. A statistical parsimony haplotype network analysis showed that the three specimens infecting an Iberian lynx corresponded to two different haplotypes, one of which was identical to a specimen in a cat found only 10. km from the lynx. Specimens from the Iberian lynx and those from cats in Doñana were only 1.03% genetically divergent, whereas specimens from Mallorca cats and those from Doñana cats and the lynx diverged by 1.33% and 1.36%, respectively. The existence of shared haplotypes of hookworms between lynx and cat reinforces the hypothesis that the abundant sympatric domestic cat population is acting as a reservoir for A. tubaeforme infection in the endangered Iberian lynx.

KW - Ancylostomiasis

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