Molecular cloning and physical map of bacteriophage PM2 DNA

Alicia Alonso, Angélica Medina, Rafael Vicuña, Alejandro Venegas, Pablo Valenzuela, Arturo Yudelevich

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

Idioma originalEnglish
Páginas (desde-hasta)115-118
Número de páginas4
PublicaciónGene
Volumen13
N.º1
DOI
EstadoPublished - 1981

Huella dactilar

Corticoviridae
Molecular Cloning
DNA
Bacteriophages
Plasmids
Genome
Lipids
Enzymes

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Citar esto

Alonso, A., Medina, A., Vicuña, R., Venegas, A., Valenzuela, P., & Yudelevich, A. (1981). Molecular cloning and physical map of bacteriophage PM2 DNA. Gene, 13(1), 115-118. https://doi.org/10.1016/0378-1119(81)90049-4
Alonso, Alicia ; Medina, Angélica ; Vicuña, Rafael ; Venegas, Alejandro ; Valenzuela, Pablo ; Yudelevich, Arturo. / Molecular cloning and physical map of bacteriophage PM2 DNA. En: Gene. 1981 ; Vol. 13, N.º 1. pp. 115-118.
@article{355aca9f116a48eca09808821f365b67,
title = "Molecular cloning and physical map of bacteriophage PM2 DNA",
abstract = "The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.",
keywords = "Alteromonas espejiana, pER322 plasmid vector, Recombinant DNA, restriction site mapping",
author = "Alicia Alonso and Ang{\'e}lica Medina and Rafael Vicu{\~n}a and Alejandro Venegas and Pablo Valenzuela and Arturo Yudelevich",
year = "1981",
doi = "10.1016/0378-1119(81)90049-4",
language = "English",
volume = "13",
pages = "115--118",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

Alonso, A, Medina, A, Vicuña, R, Venegas, A, Valenzuela, P & Yudelevich, A 1981, 'Molecular cloning and physical map of bacteriophage PM2 DNA', Gene, vol. 13, n.º 1, pp. 115-118. https://doi.org/10.1016/0378-1119(81)90049-4

Molecular cloning and physical map of bacteriophage PM2 DNA. / Alonso, Alicia; Medina, Angélica; Vicuña, Rafael; Venegas, Alejandro; Valenzuela, Pablo; Yudelevich, Arturo.

En: Gene, Vol. 13, N.º 1, 1981, p. 115-118.

Resultado de la investigación: Article

TY - JOUR

T1 - Molecular cloning and physical map of bacteriophage PM2 DNA

AU - Alonso, Alicia

AU - Medina, Angélica

AU - Vicuña, Rafael

AU - Venegas, Alejandro

AU - Valenzuela, Pablo

AU - Yudelevich, Arturo

PY - 1981

Y1 - 1981

N2 - The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

AB - The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

KW - Alteromonas espejiana

KW - pER322 plasmid vector

KW - Recombinant DNA

KW - restriction site mapping

UR - http://www.scopus.com/inward/record.url?scp=0019499077&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(81)90049-4

DO - 10.1016/0378-1119(81)90049-4

M3 - Article

VL - 13

SP - 115

EP - 118

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

Alonso A, Medina A, Vicuña R, Venegas A, Valenzuela P, Yudelevich A. Molecular cloning and physical map of bacteriophage PM2 DNA. Gene. 1981;13(1):115-118. https://doi.org/10.1016/0378-1119(81)90049-4